FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493060
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN74251.5059019186305926No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT18840.38210359793939885No Hit
GTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCAGG17590.3567517137873687No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA15090.3060479454833083No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG14470.29347341094390134No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG13690.27765383523303455No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA12910.26183425952216766No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12110.24560905366486835No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA11540.2340485944915426No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA11200.22715288200219041No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT11090.22492191619681176No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9820.19916440189834908No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9810.19896158682513287No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC9650.19571654565367297No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAATTATT9510.1928771346286456No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9360.189834908530402No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA9130.18517016184642843No Hit
TCCACAGAGTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTA8960.18172230560175232No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC8920.18091104530888735No Hit
CTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTT8900.18050541516245489No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCGTTGACATACT8750.17746318906421127No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA8730.17705755891777877No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT8540.17320407252667017No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA8520.1727984423802377No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8310.16853932584269662No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA8110.16448302437837178No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTT7980.16184642842656066No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGTACCTTGCCACAG7920.1606295379872632No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7750.15718168174258712No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT7690.15596479130328966No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7630.1547479008639922No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG7530.15271975013182978No Hit
GAGTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCA7490.15190848983896482No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTCCCAATTACATATCCCATGAA7390.14988033910680243No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7160.14521559242282886No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTCT6940.14075366081207155No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6740.13669735934774674No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6640.13466920861558432No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6600.13385794832271933No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6430.13041009207804324No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6350.1287875714923133No Hit
ATGTTAACATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC6290.12757068105301586No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTT6290.12757068105301586No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC6210.12594816046728594No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6180.1253397152476372No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6170.12513690017442097No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6120.12412282480833976No Hit
ATGTAATTGGGAGTTGGGGTACCTTGCCACAGGAACATATTGTACAAAAA6080.12331156451547479No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT6070.12310874944225854No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5880.11925526305114997No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5870.11905244797793373No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTACTGTACCTGTC5720.11601022187969011No Hit
GCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5710.11580740680647386No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5620.11398207114752769No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5570.11296799578144649No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA5550.11256236563501398No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC5530.11215673548858152No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5500.11154829026893279No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA5490.11134547519571654No Hit
GTCTGGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCC5480.11114266012250029No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5320.10789761895104044No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT5260.106680728511743No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTC5230.10607228329209428No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA5190.1052610229992293No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACA5170.10485539285279682No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5080.10303005719385064No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5070.1028272421206344No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5040.10221879690098569No Hit
AACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT4980.10100190646168825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)