FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493060
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCAGG21130.42854824970591815No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT16330.33119701456212225No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA15830.3210562609013102No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG14270.2894171094795765No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13230.2683243418650874No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG13170.26710745142578995No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA12460.2527075812274368No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA12130.24601468381130084No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA11810.23952460146838112No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11620.23567111507727256No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT10670.2164036831217296No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCGTTGACATACT10620.2153896077556484No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT10150.20585729931448504No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC9850.1997728471179978No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC9810.19896158682513287No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9410.1908489838964832No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9370.1900377236036182No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA9320.189023648237537No Hit
CTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTT9130.18517016184642843No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTCCCAATTACATATCCCATGAA8690.1762462986249138No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAATTATT8600.17442096296596762No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC8550.17340688759988643No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8460.17158155194094024No Hit
TCCACAGAGTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTA8410.17056747657485904No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGTACCTTGCCACAG8320.16874214091591286No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA8280.1679308806230479No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTT8220.16671399018375047No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA8040.16306331886585812No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA8020.16265768871942562No Hit
GAGTATGTAAATAATGTCTAGTCTGGAAGTAATGATTAACTGTATGTTCA7890.16002109276761448No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7680.1557619762300734No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA7500.15211130491218108No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7450.1510972295460999No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT7330.14866344866750497No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG7060.14318744169066644No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT7020.1423761813978015No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6880.1395367703727741No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6660.13507483876201679No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTCT6560.1330466880298544No Hit
ATGTAATTGGGAGTTGGGGTACCTTGCCACAGGAACATATTGTACAAAAA6550.13284387295663813No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6490.1316269825173407No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6490.1316269825173407No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6480.13142416744412444No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6320.1281791262726646No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6280.12736786597979963No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6260.12696223583336713No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6070.12310874944225854No Hit
ATGTTAACATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCC6050.12270311929582607No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG6030.12229748914939359No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6000.12168904392974486No Hit
GCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5880.11925526305114997No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5840.118444002758285No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5810.11783555753863628No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTACTG5780.11722711231898755No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTT5700.11560459173325761No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5630.11418488622074394No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC5630.11418488622074394No Hit
GTCTGGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCC5590.11337362592787896No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTCTATTCTTCTACTGTACCTGTC5570.11296799578144649No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC5480.11114266012250029No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT5430.1101285847564191No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5300.10749198880460795No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5290.10728917373139173No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5270.10688354358495923No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5250.10647791343852675No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA5190.1052610229992293No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG5130.10404413255993186No Hit
CTATAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA5130.10404413255993186No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5100.10343568734028313No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT5100.10343568734028313No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5070.1028272421206344No Hit
CTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT5060.10262442704741816No Hit
GCTCTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5030.10201598182776944No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACA5030.10201598182776944No Hit
CTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT5010.10161035168133696No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5000.10140753660812071No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTCTATTC4970.10079909138847198No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA4960.10059627631525574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)