FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435882
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57341.3154936427748793No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT23640.5423486172863299No Hit
GGGCCAAATCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAAT23550.5402838382865087No Hit
ACTAAATCCCTGGATGCTGGATCTTGCAAATTATTACCCACCCAGGTAGC17480.4010259657430222No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC17270.39620814807677307No Hit
GTTTTAAAGTAATGATTAACTACCTGATCAGGATAATAAGGTTTAATCCC17270.39620814807677307No Hit
ATATGATCCTGTGGCAATGTGCCCCAACTTCCAATTACATATCCCATGAA15590.3576656067467801No Hit
CTTTATCTAGAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGC14800.33954143552612864No Hit
ACTGAAGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG14790.3393120156372596No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG14250.3269233416383333No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA12480.2863160213085193No Hit
CAAAAAGATGGGGTTATTCCCTAAATTTCATGGGATATGTAATTGGAAGT11880.2725508279763789No Hit
GGATCATATTGTACAAAAAATCAAAAATTGTTTTAGAAAACTTCCTGTCA10630.24387334186775322No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10480.24043204353471812No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTAA10380.23813784464602805No Hit
CCATTTACACAATGTGGTTATCCTGCATTAATGCCTTTATATGCATGCAT10210.23423770653525494No Hit
GTCTTCAAGGAGATTGACGAGATGTGAGAGGCAATATTTGGAACAGGGTT10110.23194350764656488No Hit
CCTCGAGGACTGGGGACCCTGCGCCGAACATGGAGAACATCACATCAGGA9800.22483149109162573No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT9390.2154252756479965No Hit
CTCATAAGGTGGGAAATTTTACTGGACTTTATTCTTCTACTGTCCCTATC9240.21198397731496138No Hit
GGATCTAGTAGTCAATTATGTTAATACTAACATGGGCCTAAAGATCAGGC8580.196842264649607No Hit
CATGAAGACATTGCCAATAGATGTGAACAATTTGTAGGCCCTCTCACTGT8380.1922538668722269No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA8360.19179502709448887No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC8220.1885831486503228No Hit
ATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAA8120.18628894976163274No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTATTCTTCTACTG7900.1812417122065146No Hit
CTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG7810.17917693320669356No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG7730.1773415740957415No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7640.17527679509592045No Hit
ACTGAAGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG7610.17458853542931344No Hit
CATATAAAGGCATTAATGCAGGATAACCACATTGTGTAAATGGAGCGGCA7510.17229433654062337No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTAAAAGCCTGTT7410.17000013765193334No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTAAATTTCAT7180.1647234802079462No Hit
CTTTCAAACCCGACATTATTTACATACTCTTTGGAAGGCTGGGATTCTAT7180.1647234802079462No Hit
ATATGGTACTGGGGGCGAAGTCTGTACAACATCTTGAGTCCCTTTATACC7020.1610527619860421No Hit
CTATAGGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTC6950.15944682276395905No Hit
CTAGACAAAGGGATTAAACCTTATTATCCTGATCAGGTAGTTAATCATTA6870.157611463653007No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTTGTGCTGTT6870.157611463653007No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG6770.15531726476431695No Hit
CCTCCACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6330.14522278965408067No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTA6220.14269917087652162No Hit
ACTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTT6160.1413226515433076No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTATTC6110.14017555209896254No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA6100.13994613221009355No Hit
CCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGAC6030.13834019298801054No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA6010.1378813532102725No Hit
GTTTGAAAGTAATGATTAACTACCTGATCAGGATAATAAGGTTTAATCCC5920.13581657421045146No Hit
ATACAGCCTCAGCTCTGTATCGGGAAGCATTAGAGTCTCCTGAGCATTGC5860.13444005487723742No Hit
GGATAATAAGGTTTAATCCCTTTATCTAGAGGCAAATATTTTGTGCTGTT5820.1335223753217614No Hit
GACTTCGCCCCCAGTACCATATCATCCATATAGCTAAAAGCCAAACAGTG5660.1298516570998573No Hit
GTTTCGCTTGTATGCATGCATATAAAGGCATTAATGCAGGATAACCACAT5650.12962223721098828No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG5610.12870455765551228No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGCCTAAAGATCAGG5560.12755745821116724No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA5470.1254926792113462No Hit
GGGCGAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAAT5460.1252632593224772No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG5320.1220513808783111No Hit
GAGAATGGCTTGCCTGAGTGCTGTATGGTGAGGTGAGCAATGCTCAGGAG5310.12182196098944209No Hit
GGATAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT5290.12136312121170409No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5270.12090428143396609No Hit
GTAAACGCCTGTTTCGCTTGTATGCATGCATATAAAGGCATTAATGCAGG5210.11952776210075204No Hit
CTAGACAAAGGGATTAAACCTTATTATCCTGATCAGGTAGTTAATCATTACTTTAAAAC5200.11929834221188303No Hit
GTCCTTAAACACACAGTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACA5200.11929834221188303No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAATACACCTCCGTGTGGGG5160.11838066265640701No Hit
GCCACTCTCTGCTGGGGGGAATTAATGACTCTAGCTACCTGGGTGGGTAA5050.11585704387884796No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA4790.10989212676825379No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAAT4770.10943328699051579No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4770.10943328699051579No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA4730.10851560743503977No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT4700.10782734776843275No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTAAACGCCTGTT4640.1064508284352187No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTTAAAGTAATGATTAACTACC4620.10599198865748069No Hit
ACGTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTA4590.10530372899087367No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACC4580.10507430910200467No Hit
CTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCT4580.10507430910200467No Hit
GGGCACATTGACCACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATC4580.10507430910200467No Hit
GTTTTAAAGTAATGATTAACTACCTGATCAGGATAATAAGGTTTAATCCCTTTGTCTAG4560.10461546932426664No Hit
CTGTAAATGAAAAGAGACGACTGAAATTAATTATGCCTGCTAGGTTTTAT4540.10415662954652864No Hit
ATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTAT4520.10369778976879063No Hit
TAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCT4520.10369778976879063No Hit
GGTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGAT4510.10346836987992164No Hit
CCTCCTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGT4460.10232127043557661No Hit
CCTTGAAGACTGGGGACCCTGCGCCGAACATGGAGAACATCACATCAGGA4450.1020918505467076No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4390.10071533121349355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)