FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435882
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCCAAATCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAAT22530.5168830096218702No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT21690.4976117389568736No Hit
GTTTTAAAGTAATGATTAACTACCTGATCAGGATAATAAGGTTTAATCCC17520.40194364529849824No Hit
ATATGATCCTGTGGCAATGTGCCCCAACTTCCAATTACATATCCCATGAA17520.40194364529849824No Hit
ACTAAATCCCTGGATGCTGGATCTTGCAAATTATTACCCACCCAGGTAGC17250.3957493082990351No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC17220.3950610486324281No Hit
CTTTATCTAGAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGC14870.34114737474821166No Hit
ACTGAAGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG13440.30834033063994387No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA12550.2879219605306023No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG12310.2824158831977462No Hit
GTCTTCAAGGAGATTGACGAGATGTGAGAGGCAATATTTGGAACAGGGTT11900.27300966775411695No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11790.27048604897655787No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTAA11500.2638328721993567No Hit
CCTCCACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11030.2530501374225135No Hit
GGATCATATTGTACAAAAAATCAAAAATTGTTTTAGAAAACTTCCTGTCA10900.25006767886721637No Hit
CAAAAAGATGGGGTTATTCCCTAAATTTCATGGGATATGTAATTGGAAGT10780.2473146402007883No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT10360.23767900486829002No Hit
CCATTTACACAATGTGGTTATCCTGCATTAATGCCTTTATATGCATGCAT10270.23561422586846897No Hit
CCTCGAGGACTGGGGACCCTGCGCCGAACATGGAGAACATCACATCAGGA9490.21771947453668652No Hit
ATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAA9030.20716615964871227No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC8920.20464254087115322No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8760.20097182264924912No Hit
CTCATAAGGTGGGAAATTTTACTGGACTTTATTCTTCTACTGTCCCTATC8510.19523632542752398No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8460.19408922598317893No Hit
GGATCTAGTAGTCAATTATGTTAATACTAACATGGGCCTAAAGATCAGGC8400.1927127066499649No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTATTCTTCTACTG8360.19179502709448887No Hit
CTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG7990.18330649120633566No Hit
ACTGAAGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG7880.18078287242877658No Hit
CATGAAGACATTGCCAATAGATGTGAACAATTTGTAGGCCCTCTCACTGT7850.1800946127621696No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA7540.1729825962072304No Hit
CTATAGGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTC7260.16655883931889826No Hit
CTTTCAAACCCGACATTATTTACATACTCTTTGGAAGGCTGGGATTCTAT7070.16219986143038712No Hit
GTTTCGCTTGTATGCATGCATATAAAGGCATTAATGCAGGATAACCACAT7060.16197044154151813No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTAAAAGCCTGTT7020.1610527619860421No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7000.16059392220830407No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG6990.16036450231943508No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6870.157611463653007No Hit
CATATAAAGGCATTAATGCAGGATAACCACATTGTGTAAATGGAGCGGCA6780.15554668465318594No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG6720.15417016531997194No Hit
ATATGGTACTGGGGGCGAAGTCTGTACAACATCTTGAGTCCCTTTATACC6630.15210538632015086No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTAAATTTCAT6570.15072886698693683No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTTGTGCTGTT6500.1491229277648538No Hit
GTTTGAAAGTAATGATTAACTACCTGATCAGGATAATAAGGTTTAATCCC6470.1484346680982468No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTA6400.14682872887616374No Hit
CTAGACAAAGGGATTAAACCTTATTATCCTGATCAGGTAGTTAATCATTA6340.14545220954294968No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTATTC6190.1420109112099146No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA6100.13994613221009355No Hit
GGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA6060.13902845265461752No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG5930.13604599409932047No Hit
GGATAATAAGGTTTAATCCCTTTATCTAGAGGCAAATATTTTGTGCTGTT5760.13214585598854736No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAAAAAGTAAACGCCTGTT5720.13122817643307133No Hit
CCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGAC5660.1298516570998573No Hit
GGGCGAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAAT5500.1261809388779532No Hit
GGATAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT5480.1257220991002152No Hit
ACTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTT5450.12503383943360818No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5410.12411615987813215No Hit
GAGAATGGCTTGCCTGAGTGCTGTATGGTGAGGTGAGCAATGCTCAGGAG5370.12319848032265614No Hit
GGGCTGTATCGTCCCCTTCTCCGTCTGCCGTACCGTCCGACCACGGGGCG5320.1220513808783111No Hit
ATACAGCCTCAGCTCTGTATCGGGAAGCATTAGAGTCTCCTGAGCATTGC5310.12182196098944209No Hit
GACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCA5310.12182196098944209No Hit
GCCACTCTCTGCTGGGGGGAATTAATGACTCTAGCTACCTGGGTGGGTAA5270.12090428143396609No Hit
GTTTTAAAGTAATGATTAACTACCTGATCAGGATAATAAGGTTTAATCCCTTTGTCTAG5240.12021602176735906No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGCCTAAAGATCAGG5200.11929834221188303No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5170.11861008254527602No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA5170.11861008254527602No Hit
GTAAACGCCTGTTTCGCTTGTATGCATGCATATAAAGGCATTAATGCAGG5020.11516878421224093No Hit
CCTTGAAGACTGGGGACCCTGCGCCGAACATGGAGAACATCACATCAGGA4980.1142511046567649No Hit
GGTCTATAGGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATA4960.11379226487902688No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAAT4890.11218632565694385No Hit
AGTAAACGCCTGTTTCGCTTGTATGCATGCATATAAAGGCATTAATGCAG4870.11172748587920585No Hit
CTAGACAAAGGGATTAAACCTTATTATCCTGATCAGGTAGTTAATCATTACTTTAAAAC4830.1108098063237298No Hit
GTCCTCGAGGAGATTGACGAGATGTGAGAGGCAATATTTGGAACAGGGTT4660.10690966821295671No Hit
GACTTCGCCCCCAGTACCATATCATCCATATAGCTAAAAGCCAAACAGTG4620.10599198865748069No Hit
CTGTAAATGAAAAGAGACGACTGAAATTAATTATGCCTGCTAGGTTTTAT4610.10576256876861168No Hit
GGGCACATTGACCACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATC4540.10415662954652864No Hit
CTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCT4530.10392720965765964No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA4470.10255069032444561No Hit
TAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCT4450.1020918505467076No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT4430.10163301076896959No Hit
ACGTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTA4400.10094475110236256No Hit
GTACAATATGATCCTGTGGCAATGTGCCCCAACTTCCAATTACATATCCC4370.10025649143575555No Hit
ATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTAT4360.10002707154688655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)