FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305697
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATTTACAT12170.39810662191647284No Hit
GTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTT11860.38796586162114777No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10080.32973827024799063No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTTCCGATTG9170.29997023196171374No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT8940.29244644206518217No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGG8660.28328704566940466No Hit
TCCTCACATTCATTTGAAAGAGGATATTATCAACAGATGTCAACAATTTG8240.2695479510757384No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA7470.24435961098735023No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7450.2437053683876518No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTTTGGTTAGGATAGAAC7160.23421885069202514No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7000.22898490989443795No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG6900.22571369689594598No Hit
TTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6650.21753566439971606No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6470.21164748100243053No Hit
GGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCATTAAC6330.20706778280454174No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG5870.1920202030114787No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT5830.1907117178120819No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5780.18907611131283591No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT5690.18613201961419312No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAAC5670.18547777701449475No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT5610.18351504921539954No Hit
CTCTCAGAGACAGTCATCCTCAGGCCATCCAGTGGAACTCCAGCCCTGCT5570.18220656401600277No Hit
CGCTCGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT5500.1799167149170584No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5460.1786082297176616No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5160.1687945907221857No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5140.1681403481224873No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTT5080.16617762032339212No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT5070.1658504990235429No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5040.16486913512399531No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC5010.16388777122444773No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC4900.16028943692610656No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA4850.1586538304268606No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCCTGCCACAG4840.15832670912701138No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA4710.15407413222897184No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAACATTCATTTGAAAG4680.15309276832942426No Hit
CATCGAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT4670.15276564702957504No Hit
ATATGTTCCTGTGGCAGGGTACCCCAACTTCCAATTACATATCCCATGAA4660.15243852572972585No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA4650.15211140442987664No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC4630.15145716183017827No Hit
CATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGGGCGGCA4610.15080291923047986No Hit
CTCCAAAAGACACCAAATACTCTAAAACAGTTTCTCTTCCAAAAGTAAGA4570.14949443403108306No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCA4380.14327912933394832No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4320.14131640153485314No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4260.13935367373575797No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGAAGT4230.13837230983621038No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4200.1373909459366628No Hit
CTATTGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCAA4200.1373909459366628No Hit
GTAGTAAGCTATGTCAACGTTAACATGGGCCTAAAAATCAGACAATTATT4130.1351010968377184No Hit
CTCCCAGTACATAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAG4060.13281124773877404No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4050.13248412643892482No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA3880.12692306434148845No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3870.12659594304163926No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3850.12594170044194086No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3830.12528745784224246No Hit
CATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGGGCGGCA3810.12463321524254409No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3760.1229976087432981No Hit
ACTTTGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGA3730.12201624484375051No Hit
CTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT3680.12038063834450452No Hit
GTTCTATCCTAACCAAACCAAATATTTGCCCTTAGATAAAGGCATTAAAC3520.11514669754691738No Hit
ATCCTAACCAAACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT3480.11383821234752058No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3480.11383821234752058No Hit
CCTCTCAGAGACAGTCATCCTCAGGCCATCCAGTGGAACTCCAGCCCTGC3430.11220260584827459No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3400.111221241948727No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT3390.11089412064887781No Hit
CTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3380.11056699934902862No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3370.11023987804917941No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3350.10958563544948101No Hit
ACACAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG3320.10860427154993342No Hit
GCATCAAACCTTATTATCCTGAACATACAGTTAATCATTACTTCCAAACT3230.10566017985129066No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3220.10533305855144146No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC3210.10500593725159227No Hit
ATGTTAGGAAAAGAGGGAGTTTGCCAGTCAGGATTAAAGACAGGTACAGT3160.10337033075234628No Hit
GAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGATTCTCTCTTATA3160.10337033075234628No Hit
ACATAAATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAA3150.10304320945249706No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT3140.10271608815264788No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG3140.10271608815264788No Hit
CTCTGAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTTCCGATTG3090.10108048165340189No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA3090.10108048165340189No Hit
GTCCAAGCTGAATCCCGCGGACGACCCGTCCCGGGGCCGTTTGGGACTCT3080.1007533603535527No Hit
TGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCG3080.1007533603535527No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT3080.1007533603535527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)