FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305697
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTT13370.4373611778983765No Hit
ATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATTTACAT11230.36735721973064833No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTTCCGATTG9860.3225416016513083No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTTTGGTTAGGATAGAAC8300.2715106788748336No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGG8030.2626784037789053No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7500.24534097488689782No Hit
TCCTCACATTCATTTGAAAGAGGATATTATCAACAGATGTCAACAATTTG7360.24076127668900904No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT7220.23618157849112031No Hit
CGCTCGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT7040.23029339509383476No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6930.22669506079549356No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG6900.22571369689594598No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6620.21655430050016847No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6560.2145915727010733No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT6410.20968475320333532No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA6380.20870338930378773No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG6330.20706778280454174No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCCTGCCACAG5850.1913659604117803No Hit
GGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCATTAAC5800.18973035391253432No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTT5740.1877676261134391No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5720.1871133835137407No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5650.18482353441479635No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5600.18318792791555036No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT5570.18220656401600277No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5540.18122520011645518No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5380.17599125931886803No Hit
GTCCAAGCTGAATCCCGCGGACGACCCGTCCCGGGGCCGTTTGGGACTCT5350.17500989541932044No Hit
CTCTCAGAGACAGTCATCCTCAGGCCATCCAGTGGAACTCCAGCCCTGCT5280.17272004632037607No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA5000.16356064992459854No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC5000.16356064992459854No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4900.16028943692610656No Hit
ATATGTTCCTGTGGCAGGGTACCCCAACTTCCAATTACATATCCCATGAA4900.16028943692610656No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4770.15603686002806702No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC4740.15505549612851943No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC4720.15440125352882103No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4670.15276564702957504No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCA4590.15014867663078146No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT4560.14916731273123388No Hit
TGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCG4560.14916731273123388No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAAC4350.14229776543440073No Hit
CTCCAAAAGACACCAAATACTCTAAAACAGTTTCTCTTCCAAAAGTAAGA4340.14197064413455154No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAACATTCATTTGAAAG4330.14164352283470233No Hit
CCTCTCAGAGACAGTCATCCTCAGGCCATCCAGTGGAACTCCAGCCCTGC4150.1357553394374168No Hit
TTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4120.1347739755378692No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4050.13248412643892482No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGAAGT4040.13215700513907563No Hit
CATCGAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT4040.13215700513907563No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4020.13150276253937723No Hit
CTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT4010.13117564123952805No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA4000.13084851993967883No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA3980.13019427733998043No Hit
GTAGTAAGCTATGTCAACGTTAACATGGGCCTAAAAATCAGACAATTATT3950.12921291344043284No Hit
CATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGGGCGGCA3920.12823154954088525No Hit
GTTCTATCCTAACCAAACCAAATATTTGCCCTTAGATAAAGGCATTAAAC3880.12692306434148845No Hit
CTATTGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCAA3870.12659594304163926No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3770.12332473004314728No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3770.12332473004314728No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3740.1223433661435997No Hit
CTCTGAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTTCCGATTG3600.11776366794571094No Hit
CATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGGGCGGCA3550.11612806144646497No Hit
CTCTAAAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3480.11383821234752058No Hit
CTCCCAGTACATAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAG3460.11318396974782219No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3380.11056699934902862No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3380.11056699934902862No Hit
GAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGATTCTCTCTTATA3360.10991275674933022No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3340.10925851414963182No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3280.10729578635053665No Hit
ATCCTAACCAAACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT3250.10631442245098906No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCCGG3240.10598730115113986No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT3230.10566017985129066No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA3190.10435169465189387No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3150.10304320945249706No Hit
GTCTACGTCCCGTCCAAGCTGAATCCCGCGGACGACCCGTCCCGGGGCCG3140.10271608815264788No Hit
CTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3110.10173472425310029No Hit
ATGTTAGGAAAAGAGGGAGTTTGCCAGTCAGGATTAAAGACAGGTACAGT3100.1014076029532511No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCAAAG3080.1007533603535527No Hit
CTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT3080.1007533603535527No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG3070.10042623905370351No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT3060.1000991177538543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)