FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569873
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29930.5252047385996529No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG21660.3800846855351982No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19530.3427079366806288No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA17010.2984875577540961No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG16020.2811152660329582No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG15830.277781189844053No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG14710.25812768809892733No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14690.25777673271062146No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT14270.25040666955619933No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA13400.2351401101648964No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA11440.2007464821109265No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC10950.192148075097434No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC10600.18600635580208222No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT10340.1814439357541066No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTACAA9860.17302100643476703No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9830.17249457335230833No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA9540.167405720221874No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9510.16687928713941527No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9400.1649490325037333No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9380.16459807711542748No Hit
GTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAA9100.15968470167914606No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8690.15249011621887684No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA8590.15073533927734775No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT8520.14950699541827742No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8420.14775221847674833No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTGTAGGAAGTTTCCGAAAAC8380.14705030770013672No Hit
CATCTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8300.14564648614691342No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8240.144593619981996No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA8220.14424266459369017No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCGCATTGGGTAAAA8180.14354075381707854No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA8020.14073311071063202No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7650.13424043602697444No Hit
GTCTATTTGTAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATA7590.133187569862057No Hit
CAATAGGTCTATTTGTAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT7170.12581750670763486No Hit
GGATTAAACACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC7150.12546655131932904No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA6910.12125508665965926No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT6860.12037769818889472No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6810.1195003097181302No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT6790.11914935432982438No Hit
TCCTCACATTCATTTGAAAGAGGATATTATCAACAGATGTCAACAATTTG6700.1175700550824482No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG6600.11581527814091912No Hit
GGGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT6350.11142833578709642No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6310.1107264250104848No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6220.10914712576310863No Hit
GTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACAG6210.1089716480689557No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6170.1082697372923441No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6090.10686591573912081No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6060.1063394826566621No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6050.1061640049625092No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6050.1061640049625092No Hit
GGTCTATTTGTAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT6030.10581304957420339No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5980.10493566110343884No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5970.10476018340928592No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5930.10405827263267431No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT5900.10353183955021557No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT5770.10125062952622778No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5750.10089967413792196No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5740.10072419644376907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)