FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569873
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29870.5241518724347355No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG21100.37025793466263535No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG19490.3420060259040172No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA16010.28093978833880534No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT15070.264444885088432No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG14840.2604088981229151No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG14550.2553200449924808No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT13850.2430366064017772No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13270.23285890014090857No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA12690.22268119388003993No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA11710.20548437985305495No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC10760.18881399890852874No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10210.17916272573011882No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC10140.17793438187104846No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9610.16863406408094433No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9570.16793215330433273No Hit
GTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAA9560.16775667561017982No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA9400.1649490325037333No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9390.16477355480958036No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTACAA9210.16161495631482806No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9070.15915826859668733No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9030.1584563578200757No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8680.15231463852472393No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8170.14336527612292563No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCGCATTGGGTAAAA8080.14178597687554947No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA7940.13932928915740875No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA7910.13880285607495005No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTGTAGGAAGTTTCCGAAAAC7830.13739903452172678No Hit
CATCTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7720.13546877988604478No Hit
GTCTATTTGTAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATA7530.13213470369713953No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT7530.13213470369713953No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7290.12792323903746974No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7050.12371177437779997No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7020.12318534129534123No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7010.12300986360118833No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA6710.11774553277660109No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG6660.11686814430583656No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6650.11669266661168366No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6650.11669266661168366No Hit
GGATTAAACACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6550.11493788967015459No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6510.11423597889354295No Hit
GGGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT6500.11406050119939005No Hit
TCCTCACATTCATTTGAAAGAGGATATTATCAACAGATGTCAACAATTTG6440.11300763503447259No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6420.11265667964616677No Hit
GCCCAGAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6300.11055094731633189No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6270.11002451423387316No Hit
GTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACAG6180.108445214986497No Hit
CAATAGGTCTATTTGTAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT6160.10809425959819118No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA6120.10739234882157955No Hit
GGTCTATTTGTAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT6040.10598852726835628No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6040.10598852726835628No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC5950.1044092280209801No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5910.10370731724436849No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAACGTGAAACCACA5870.10300540646775685No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT5710.10019776336131032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)