FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510963
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT25150.4922078506662909No Hit
GGATAATAAGGTTTAATACCTTTGTCTAAAGGCAAATATTTTGTGCTGTT21640.42351403134864957No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA19600.3835894184119007No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19420.3800666584468934No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA17050.33368365224096463No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16680.32644242342400526No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16320.3193969034939908No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG15890.31098142135536233No Hit
GTGTAAATGGAGCGGCGAAGCCCAAAAGACCCACAATTCTCTGACATACT15570.30471873697312724No Hit
TTCTTCTACTGTCCCTATCTTTAACCCTGAATGGCAAACCCCTTCTTTTC15210.2976732170431127No Hit
CATGAAGACATTGCTAATAGATGTCAACAATTTGTAGGCCCTCTCACTGC15080.2951290015128297No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA14830.2902362793392085No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT14310.2800594172180764No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA13970.27340531506195165No Hit
GTTTAATACCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAAC13830.2706653906447238No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA13290.26009711074970204No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG13000.2544215530283015No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12990.2542258441413566No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG11970.2342635376729822No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT11670.22839227106463675No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT11110.21743257339572533No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT11060.21645402896100108No Hit
GCTAATAGATGTCAACAATTTGTAGGCCCTCTCACTGCAAATGAAAAAAG10660.20862567348320718No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC9960.1949260513970679No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT9700.18983762033650187No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC9530.18651056925843945No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA9130.17868221378064555No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG9080.17770366934592133No Hit
GTATTAAACCTTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACC8870.17359378272007953No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA8850.17320236494618985No Hit
ATCTTTAACCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACA8800.1722238205114656No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA8500.1663525539031202No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA8410.16459117392061656No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8390.16419975614672686No Hit
ATGTAAGACCTTGGGCAGGACTTGATGGGCGTTCACGGTGGTCTCCATGC8160.1596984517469954No Hit
CTTTAACCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG8150.15950274286005053No Hit
GTCCCTATCTTTAACCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA8060.15774136287754692No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG7770.15206580515614632No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7730.15128296960836696No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC7620.14913017185197364No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7500.14678166520863545No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTGC7460.1459988296608561No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7380.1444331585652973No Hit
TGCTAATAGATGTCAACAATTTGTAGGCCCTCTCACTGCAAATGAAAAAA7330.14345461413057306No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAACCCTGAATGGCAAACCCCTT7310.14306319635668335No Hit
CTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTG7300.1428674874697385No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT7230.14149752526112458No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG7090.1387576008438967No Hit
CTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCTAAAAC6890.13484342310499978No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC6890.13484342310499978No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG6800.13308204312249614No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC6750.1321034986877719No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6740.13190778980082707No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6730.1317120809138822No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT6720.13151637202693736No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG6620.1295592831574889No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC6600.1291678653835992No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCGAAGCCCAAAAGACCCACAAT6600.1291678653835992No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT6500.12721077651415075No Hit
GGGCTGTATCGTCCCCTTCTCCGTCTGCCGTACCGTCCGACCACGGGGCG6420.12564510541859195No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT6390.1250579787577574No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCATTCAGGGT6340.12407943432303317No Hit
GCTCTATATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA6330.12388372543608833No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT6310.12349230766219862No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6230.12192663656663985No Hit
ATACAGCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG6210.12153521879275016No Hit
TCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT6180.12094809213191561No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT6140.12016525658413624No Hit
TTGCTAATAGATGTCAACAATTTGTAGGCCCTCTCACTGCAAATGAAAAA6140.12016525658413624No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT6080.11899100326246714No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT6010.11762104105385322No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5990.11722962327996353No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA5950.11644678773218413No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA5940.11625107884523929No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5930.11605536995829445No Hit
GATCAGGATAATAAGGTTTAATACCTTTGTCTAAAGGCAAATATTTTGTG5880.11507682552357021No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCAGGGTCCCGTG5870.11488111663662534No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5870.11488111663662534No Hit
GGTTTAATACCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAA5850.11448969886273565No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA5850.11448969886273565No Hit
GTTTTAGAAAACTTCCTGTCAATCGACCTATTGATTGGAAAGTATGTCAG5820.11390257220190111No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5780.11311973665412173No Hit
GTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTACCCCAACTTC5720.11194548333245263No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC5690.1113583566716181No Hit
CTACATGATCAGGATAATAAGGTTTAATACCTTTGTCTAAAGGCAAATAT5680.11116264778467326No Hit
GTGCCAGCAGTGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCA5600.10959697668911447No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG5580.10920555891522478No Hit
GTACAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCC5530.10822701448050054No Hit
GGGTTATTCCCTGAATTTCATGGGTTATGTAATTGGAAGTTGGGGTACAT5530.10822701448050054No Hit
ACCTAGCAGCCATGGAAATGAAGTATATTTCCGAGAAAGGACGACAGAAT5500.107639887819666No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGACTTGATGGGCGTTC5500.107639887819666No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA5460.1068570522718866No Hit
GTCCCGTGCTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC5440.10646563449799692No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5400.10568279895021752No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5400.10568279895021752No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC5380.10529138117632783No Hit
CTCTAAGGCTTCTCGATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGA5370.10509567228938299No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA5340.10450854562854844No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5330.10431283674160359No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTAATCTCCTCC5300.10372571008076906No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5290.10353000119382422No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT5260.10294287453298967No Hit
GCACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG5230.10235574787215514No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT5210.10196433009826544No Hit
CAGTTGGACCCTGCATTCGGAGCCAATTCAAACAATCCAGATTGGGACTT5190.10157291232437574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)