FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510963
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT24220.4740069241804201No Hit
GGATAATAAGGTTTAATACCTTTGTCTAAAGGCAAATATTTTGTGCTGTT22420.4387793245303476No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19270.37713102514272073No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA18420.3604957697524087No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17980.3518845787268354No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA17400.3405334632840343No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16050.3141127635464799No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG15760.30843720582507933No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA15720.3076543702772999No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA15440.30217452144284423No Hit
TTCTTCTACTGTCCCTATCTTTAACCCTGAATGGCAAACCCCTTCTTTTC14880.29121482377393276No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT14830.2902362793392085No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG14430.28240792386141467No Hit
GTTTAATACCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAAC14310.2800594172180764No Hit
GTGTAAATGGAGCGGCGAAGCCCAAAAGACCCACAATTCTCTGACATACT14000.2739924417227862No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13840.27086109953166865No Hit
CATGAAGACATTGCTAATAGATGTCAACAATTTGTAGGCCCTCTCACTGC13780.26968684620999955No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA12910.2526601730457978No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT12110.23700346209021006No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT11470.22447809332573984No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG11410.22330384000407072No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC10240.2004059002315236No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT10200.19962306468374424No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA9660.18905478478872245No Hit
GCTAATAGATGTCAACAATTTGTAGGCCCTCTCACTGCAAATGAAAAAAG9480.18553202482371522No Hit
GGGCTGTATCGTCCCCTTCTCCGTCTGCCGTACCGTCCGACCACGGGGCG9360.18318351818037706No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA9240.18083501153703888No Hit
GTATTAAACCTTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACC9190.17985646710231465No Hit
ATCTTTAACCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACA9140.1788779226675904No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT8980.17574658047647285No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8770.17163669385063107No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8770.17163669385063107No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA8670.1696796049811826No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC8490.16615684501617534No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA8280.16204695839033353No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8000.15656710955587783No Hit
ATACAGCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG7810.15284864070392573No Hit
GTCCCTATCTTTAACCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA7780.15226151404309118No Hit
CTTTAACCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG7520.14717308298252516No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT7440.14560741188696638No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG7440.14560741188696638No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTGC7420.14521599411307667No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC7400.144824576339187No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG7170.14032327193945549No Hit
CTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTG7160.14012756305251065No Hit
CTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCTAAAAC7120.13934472750473126No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7100.13895330973084158No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA7050.13797476529611732No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC7020.1373876386352828No Hit
TGCTAATAGATGTCAACAATTTGTAGGCCCTCTCACTGCAAATGAAAAAA6990.13680051197444823No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAACCCTGAATGGCAAACCCCTT6910.13523484087888946No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA6890.13484342310499978No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6790.1328863342355513No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC6750.1321034986877719No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT6630.12975499204443375No Hit
TTGCTAATAGATGTCAACAATTTGTAGGCCCTCTCACTGCAAATGAAAAA6510.1274064854010956No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6500.12721077651415075No Hit
GCTCTATATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA6410.1254493965316471No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC6370.12466656098386773No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCATTCAGGGT6360.12447085209692287No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG6350.12427514320997803No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT6280.12290518100136409No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT6230.12192663656663985No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA6220.12173092767969501No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG6210.12153521879275016No Hit
GTTTTAGAAAACTTCCTGTCAATCGACCTATTGATTGGAAAGTATGTCAG6170.12075238324497077No Hit
GTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTACCCCAACTTC6050.1184038766016326No Hit
GATCAGGATAATAAGGTTTAATACCTTTGTCTAAAGGCAAATATTTTGTG6040.11820816771468776No Hit
GTACAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCC5990.11722962327996353No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCGAAGCCCAAAAGACCCACAAT5960.11664249661912898No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA5960.11664249661912898No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT5960.11664249661912898No Hit
CTACATGATCAGGATAATAAGGTTTAATACCTTTGTCTAAAGGCAAATAT5940.11625107884523929No Hit
ATGTAAGACCTTGGGCAGGACTTGATGGGCGTTCACGGTGGTCTCCATGC5910.11566395218440474No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5910.11566395218440474No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5890.11527253441051505No Hit
GGGTTATTCCCTGAATTTCATGGGTTATGTAATTGGAAGTTGGGGTACAT5880.11507682552357021No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAGTG5870.11488111663662534No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT5860.1146854077496805No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5850.11448969886273565No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT5850.11448969886273565No Hit
GGTTTAATACCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAA5820.11390257220190111No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT5770.11292402776717687No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA5760.11272831888023203No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA5750.1125326099932872No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5750.1125326099932872No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC5750.1125326099932872No Hit
TCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT5730.1121411922193975No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5660.11077123001078355No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5610.10979268557605931No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC5580.10920555891522478No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGTATTAAAC5530.10822701448050054No Hit
ACATAGAGTTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCAGGGTCCCGTG5480.1072484700457763No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG5480.1072484700457763No Hit
GTCCCGTGCTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC5470.10705276115883147No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5460.1068570522718866No Hit
GTGCCAGCAGTGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCA5450.10666134338494176No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA5380.10529138117632783No Hit
CCCCAAAACACCTCCGTGAGGGGGAGTGAACCCTGGCCCGAATGCTCCCA5350.10470425451549328No Hit
CTGCTGGCACTGTGGCCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAA5330.10431283674160359No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5280.10333429230687938No Hit
TTGTAGGCCCTCTCACTGCAAATGAAAAAAGAAGACTGAAATTAATTATG5270.10313858341993451No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5240.10255145675909998No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5220.10216003898521027No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT5210.10196433009826544No Hit
CTCTAAGGCTTCTCGATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGA5170.10118149455048604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)