FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374959
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT26580.7088775039404308No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT18190.4851197064212354No Hit
TTCTTCTACTGTCCCTATCTTTAATCCAGAATGGCAAACCCCTTCTTTTC15440.4117783544334181No Hit
GTGCTGTATGGTGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCCCGA14450.3853754677178038No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14020.3739075472251633No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATACCCCATGAA13920.3712405889710608No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13500.36003936430383054No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACAGTAAATGAAAAAAG13420.3579057977005486No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT12170.32456881952426797No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG11920.3179014238890119No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA11770.3139009865078582No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT11540.30776698252342255No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC11030.2941654954275001No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC10870.2898983622209362No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10560.2816307916332186No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG10430.2781637459028854No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT10410.2776303542520649No Hit
ATATAGAGCTGAGGCTGTGTCTAGTAGATCCCGGACGGAAGGAAAGAAGT10090.269096087838937No Hit
GTATACATGCATATAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT9740.25976173394957847No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG9530.2541611216159634No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT9420.25122746753645064No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG9320.2485605092823482No Hit
CATTTAAACCCTAACAAAACAAAGAAATGGGGTTATTCCCTGAATTTCAT9220.24589355102824575No Hit
GGATCATATTGTACAAAAAATCAAACACTATTTTAGAAAACTTCCTGTCA9170.24456007190119455No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8960.23895945956757939No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT8910.23762598044052816No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA8820.23522571801183595No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACAGT8710.2322920639323233No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA8260.22029075178886226No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTATATGCATGTAT8100.21602361858229832No Hit
CTCCGAAGGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8030.21415674780442662No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC7970.21255657285196516No Hit
GTCCCTATCTTTAATCCAGAATGGCAAACCCCTTCTTTTCCTAAAATTCA7730.20615587304211924No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7500.20002186905768363No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCTGTGTCTAGTAGATCCCGGA7460.19895508575604265No Hit
ACTGTGGAATTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG7370.19655482332735044No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC7360.19628812750194022No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA7330.1954880400257095No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGGTAAAACCTAGCAGGCATAA7240.19308777759701728No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG7130.19015412351750458No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6980.18615368613635092No Hit
CTTTAATCCAGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG6910.18428681535847918No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC6720.17921959467568455No Hit
CTACTAGACACAGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA6710.1789528988502743No Hit
GTCTAGTAGATCCCGGACGGAAGGAAAGAAGTCAGAAGGCAAAAATGAGA6600.1760192447707616No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC6370.16988524078632597No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATCCAGAATGGCAAACCCCTT6360.16961854496091572No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA6000.16001749524614692No Hit
GTATGGTGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCCCGATATAG5990.15975079942073667No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5800.15468357873794203No Hit
CCTCCAAGCTGTGCCTTGGATGGCTTTGGGGCATGGACATTGACCCTTAT5760.15361679543630105No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG5690.15174992465842932No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA5680.15148322883301907No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5620.14988305388055762No Hit
CTCTCACAGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGG5580.14881627057891664No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5480.14614931232481418No Hit
GGGCTGTATCGTCCCCTTCTCCGTCTGCCGTACCGTCCGACCACGGGGCG5470.14588261649940393No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5400.14401574572153222No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAATTACTCTCATTT5290.14108209164201954No Hit
CTTCTACTGTCCCTATCTTTAATCCAGAATGGCAAACCCCTTCTTTTCCT5030.13414800018135317No Hit
ATACAGCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG5010.13361460853053267No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT4910.1309476502764302No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG4860.12961417114937898No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT4840.1290807794985585No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCATTCTGGAT4760.12694721289527655No Hit
GGGTAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC4740.12641382124445605No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT4610.12294677551412288No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAGGAGACCAAATACTCAAGGA4600.12268007968871263No Hit
TTGTAGGCCCTCTCACAGTAAATGAAAAAAGAAGACTGAAATTAATTATG4470.11921303395837944No Hit
GTGCCAGCAGTGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCA4400.11734616318050774No Hit
CATGTATACAAGCGAAACAGGCCTTTACTTTCTCGCCAACTTACAAGGCC4400.11734616318050774No Hit
CTGCTGGCACTGTGGCCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAA4390.1170794673550975No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT4350.11601268405345652No Hit
GAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCA4350.11601268405345652No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT4310.11494590075181554No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA4300.11467920492640529No Hit
GGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGGTAAAA4270.11387911745017457No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAATTACTCTCATTTTTGCCTTCT4260.11361242162476431No Hit
GCCTTGGATGGCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGA4240.11307902997394381No Hit
CCTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4180.11147885502148236No Hit
GTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGGTAAAAC4170.11121215919607211No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA4150.1106787675452516No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4100.10934528841820039No Hit
CCCTTATAAAGAATTTGGAGCTACTGTGGAATTACTCTCATTTTTGCCTT4080.1088118967673799No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG4050.10801180929114916No Hit
ATGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGGCGCTAC4050.10801180929114916No Hit
CTTGTATACATGCATATAAAGGCATTAATGCAGGGTAACCACATTGTGTA4040.10774511346573892No Hit
GTTTTACCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC4030.10747841764032869No Hit
ATGGTGAGGTGAGCAATGCTCAGGAGACTCTAAGGCTTCCCGATATAGAG3920.10454476356081598No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCGAAACAGGCCTTTACTTT3910.10427806773540572No Hit
CATATAAAGGCATTAATGCAGGGTAACCACATTGTGTAAATGGAGCGGCA3910.10427806773540572No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3910.10427806773540572No Hit
CCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA3880.103477980259175No Hit
ACGTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTA3870.10321128443376475No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT3850.10267789278294427No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT3800.10134441365589304No Hit
ATATGGATGATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCC3750.10001093452884181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)