FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633760
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30570.48235925271396113No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA18240.28780610956829084No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT18140.28622822519565766No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA16170.2551439030547842No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15960.25183034587225445No Hit
CTCCAAAAGATACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA15720.2480434233779349No Hit
GGATATTATCAATAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG15230.2403117899520323No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG14670.23147563746528654No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG14560.22973996465539007No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA14030.22137717748043426No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTGTGTACAATATGTT13490.2128566018682151No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12400.19565766220651348No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC11450.18066776066649837No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT11180.1764074728603888No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT10950.1727783388033325No Hit
TATCAATAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAA10810.17056930068164605No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT10770.16993814693259277No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATTAGACAATTATT10370.1636266094420601No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG10310.16267987881848017No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA10100.15936632163595052No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA10080.1590507447614239No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC9870.1557371875788942No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9810.1547904569553143No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9670.15258141883362789No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACGTATAAGGCC9400.14832113102751832No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTATTGATAATATCCTCTTTT9330.1472166119666751No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA9320.14705882352941177No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9200.14516536228225194No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8810.1390116132289826No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8470.1336468063620298No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAATTTCAT8300.13096440292855338No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC8020.1265463266851805No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA7970.12575738449886392No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAATAGACGGAAA7960.1255995960616006No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG7940.12528401918707396No Hit
GTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCTC7930.12512623074981066No Hit
ATTATCAATAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA7880.12433728856349406No Hit
GCCTTATACGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACATGC7810.12323276950265084No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATACGTTGGCGA7740.12212825044180763No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7670.1210237313809644No Hit
CAATAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAAGGA7630.12039257763191115No Hit
GGAACATATTGTACACAAATTGAAACAATGTTTTCGGAAACTTCCTATAA7550.1191302701338046No Hit
ATGTTAACATGGGCCTAAAAATTAGACAATTATTGTGGTTTCACATTTCC7530.11881469325927796No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7500.118341327947488No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT7490.1181835395102247No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7470.11786796263569806No Hit
CTTAAAAGAGGATATTATCAATAGATGTCAACAATTTGTAGGCCCTCTTA7470.11786796263569806No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7470.11786796263569806No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7380.11644786670032821No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA7320.1155011360767483No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7160.11297652108053521No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC7140.11266094420600858No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7100.11202979045695531No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA7040.1110830598333754No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7040.1110830598333754No Hit
CTTTTGGAGTGTGGATTCGCACTCCACCCGCTTACAGACCACCAAATGCC7010.11060969452158546No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7000.11045190608432215No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCACTTAAAAG6990.11029411764705882No Hit
GTTCTAGAGTATTTGGTATCTTTTGGAGTGTGGATTCGCACTCCACCCGC6870.10840065639989901No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6830.10776950265084574No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG6760.10666498359000252No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6650.10492931078010603No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTGTGTACAAT6610.10429815703105276No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTGTC6500.10256248422115628No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAA6430.10145796516031304No Hit
AATTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA6420.10130017672304974No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG6410.10114238828578641No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6350.10019565766220652No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT6340.10003786922494319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)