FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633760
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30460.4806235799040646No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG17830.2813367836404948No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT17290.27281620802827566No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16960.2676091895985862No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA16500.2603509214844736No Hit
CTCCAAAAGATACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA16380.2584574602373138No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG15360.24236303963645545No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA15220.240154001514769No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14940.2357359252713961No Hit
GGATATTATCAATAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG14750.2327379449633931No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13500.21301439030547842No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTGTGTACAATATGTT13220.20859631406210552No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT11360.1792476647311285No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC11070.1746718000504923No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT10830.17088487755617268No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC10710.16899141630901288No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA10500.1656778591264832No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATTAGACAATTATT10270.1620487250694269No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA10160.1603130522595304No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG9940.15684170663973745No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9710.15321257258268114No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9600.15147689977278464No Hit
TATCAATAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAA9530.15037238071194142No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTATTGATAATATCCTCTTTT9440.14895228477657158No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9440.14895228477657158No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9420.14863670790204495No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9210.14532315071951526No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT8850.13964276697803585No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8820.1391694016662459No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG8760.13822267104266597No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG8600.1356980560464529No Hit
GTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCTC8510.13427796011108306No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8430.13301565261297651No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8300.13096440292855338No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8220.12970209543044686No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7980.12591517293612722No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7960.1255995960616006No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG7900.12465286543802069No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACGTATAAGGCC7880.12433728856349406No Hit
GGAACATATTGTACACAAATTGAAACAATGTTTTCGGAAACTTCCTATAA7810.12323276950265084No Hit
GCCTTATACGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACATGC7780.1227594041908609No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7690.12133930825549102No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7620.12023478919464782No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATACGTTGGCGA7620.12023478919464782No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAATTTCAT7540.11897248169654127No Hit
ATGTTAACATGGGCCTAAAAATTAGACAATTATTGTGGTTTCACATTTCC7490.1181835395102247No Hit
CTTTTGGAGTGTGGATTCGCACTCCACCCGCTTACAGACCACCAAATGCC7480.11802575107296137No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA7290.11502777076495833No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG7280.11486998232769503No Hit
ATTATCAATAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA7210.11376546326685182No Hit
GTTCTAGAGTATTTGGTATCTTTTGGAGTGTGGATTCGCACTCCACCCGC7080.11171421358242867No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG7080.11171421358242867No Hit
CAATAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAAGGA6970.10997854077253219No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6950.10966296389800555No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA6950.10966296389800555No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA6850.10808507952537237No Hit
CTTAAAAGAGGATATTATCAATAGATGTCAACAATTTGTAGGCCCTCTTA6740.10634940671547588No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCACTTAAAAG6680.10540267609189598No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6660.10508709921736935No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6660.10508709921736935No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTGTGTACAAT6550.10335142640747286No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAA6490.10240469578389295No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6460.10193133047210301No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG6450.10177354203483968No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6430.10145796516031304No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6410.10114238828578641No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6380.10066902297399646No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAATAGACGGAAA6350.10019565766220652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)