FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500113
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28400.567871661004613No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA26530.5304801114948022No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14830.29653298354571866No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14320.2863352882248612No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12020.24034568187589603No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10970.2193504268035424No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10440.20875282186225913No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT10330.20655331894991732No Hit
CTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10280.20555354489885286No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10070.20135449388438212No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9770.19535584957799537No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8930.17855964552011247No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8860.1771599618486222No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG8850.1769600070384093No Hit
GGATAATAAGGTTTAATGCCCTTATCCAATGGCAAATATTTGGTAACATT8400.16796204057882919No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8390.1677620857686163No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC8340.16676231171755182No Hit
GGATAAGGGCATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8140.16276321551329398No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT7880.15756439044775883No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7710.15416515867413963No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7630.15256552019243652No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7530.1505659720903076No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT7410.14816651436775288No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7410.14816651436775288No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7310.14616696626562398No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7040.14076818638987587No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6980.13956845752859853No Hit
CTCACAGTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT6830.13656913537540516No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6790.1357693161345536No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6730.13456958727327625No Hit
GCCTAGCAGCCATGGAAACGACGTATATTTGCGGGAGAGAACAACAGAGT6600.13197017474050865No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6560.13117035549965708No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC6430.12857094296688948No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6380.12757116891582504No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6230.12457184676263164No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6110.12217238904007695No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA5990.11977293131752223No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5940.11877315726645779No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5910.11817329283581911No Hit
GTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTACCC5850.11697356397454176No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC5760.11517397068262572No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5750.11497401587241282No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT5680.1135743322009226No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5620.11237460333964525No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC5540.1107749648579421No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5400.10797559751496162No Hit
GATTATGCCTGCTAGGTTTTACCCAAATGTTACCAAATATTTGCCATTGG5390.10777564270474872No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5390.10777564270474872No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5350.10697582346389715No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5340.10677586865368426No Hit
CTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5310.10617600422304559No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA5290.10577609460261982No Hit
ACCCAAATGTTACCAAATATTTGCCATTGGATAAGGGCATTAAACCTTAT5270.10537618498219403No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5250.10497627536176825No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5230.10457636574134246No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5160.10317668206985221No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5130.10257681763921353No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC5110.10217690801878775No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5100.10197695320857485No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5090.10177699839836198No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5070.10137708877793619No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTACCCAAATGTTA5050.10097717915751041No Hit
GTTCTGGATAATAAGGTTTAATGCCCTTATCCAATGGCAAATATTTGGTA5030.10057726953708462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)