FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500113
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28330.5664719773331227No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA25950.5188827325024544No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG13740.27473790923251346No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13510.27013894859761695No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12830.2565420215031403No Hit
CTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11130.2225497037669487No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11050.22095006528524552No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10290.20575349970906576No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10210.20415386122736262No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9420.18835743122054416No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9280.18555806387756366No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9240.1847582446367121No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG9030.18055919362224138No Hit
GGATAATAAGGTTTAATGCCCTTATCCAATGGCAAATATTTGGTAACATT8520.1703614983013839No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8460.16916176944010652No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT8050.16096362222137797No Hit
GGATAAGGGCATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8000.1599638481703135No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7480.14956619803924312No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7270.14536714702477238No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7200.14396746335328217No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7200.14396746335328217No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC7140.1427677344920048No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6880.1375689094264696No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6800.1359692709447665No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6720.13436963246306335No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6540.1307704458792313No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6530.1305704910690184No Hit
CTCACAGTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT6520.13037053625880551No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6420.12837098815667658No Hit
GTCAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTACCC6230.12457184676263164No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6230.12457184676263164No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6210.12417193714220585No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA6180.12357207271156717No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6170.1233721179013543No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6050.1209726601787996No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC5970.11937302169709645No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5930.11857320245624489No Hit
GTTCTGGATAATAAGGTTTAATGCCCTTATCCAATGGCAAATATTTGGTA5690.11377428701113548No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5610.11217464852943235No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC5570.11137482928858078No Hit
GCCTAGCAGCCATGGAAACGACGTATATTTGCGGGAGAGAACAACAGAGT5560.11117487447836788No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5380.10757568789453584No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5290.10577609460261982No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5240.10477632055155535No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC5230.10457636574134246No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTGACTGTGAGTGGGCCTAC5200.10397650131070378No Hit
CTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5180.103576591690278No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5100.10197695320857485No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5050.10097717915751041No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5040.10077722434729752No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5020.10037731472687172No Hit
GATTATGCCTGCTAGGTTTTACCCAAATGTTACCAAATATTTGCCATTGG5010.10017735991665884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)