FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356945
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16010.4485284847805684No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15160.4247152922719186No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA13840.3877348050820154No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC13580.38045076972642844No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC10750.30116684643292385No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTAT8860.24821751250192606No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8800.24653658126602135No Hit
CTACTATACCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAAT8310.23280897617279972No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAG8100.2269257168471333No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7920.22188292313941924No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTATGGGTCATCTTATT7560.2117973357239911No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC7450.20871562845816582No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG7240.2028323691324994No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6810.19078569527518247No Hit
GAATCAGAGGACAGACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC6770.18966507445124597No Hit
GTTTTGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGTAGTC6650.1863032119794366No Hit
ACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA6650.1863032119794366No Hit
GTTCATCGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGGGAGAGG6650.1863032119794366No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6540.18322150471361134No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC6490.18182072868369076No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6400.17929933182983374No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6400.17929933182983374No Hit
CATTTAAACCCTAACAAAACCAAAAGATGGGGTTACTCTTTAAATTTCAT6380.1787390214178655No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT6180.1731359172981832No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6160.17257560688621496No Hit
CTTGTGTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG6020.16865343400243735No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG5970.16725265797251676No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5920.1658518819425962No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG5900.16529157153062798No Hit
AACTACTGTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT5890.16501141632464386No Hit
ATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATTC5730.160528933028898No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTAAAAGCCAAA5640.15800753617504099No Hit
GTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTCTCTCAAAGGTGG5630.15772738096905686No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5590.1566067601451204No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTATGGGTCATCTTA5490.15380520808527923No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5440.15240443205535867No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5400.1512838112314222No Hit
CTGTACGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC5190.1454005519057558No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5170.14484024149378757No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT5160.14456008628780345No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA5120.14343946546386696No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5080.14231884463993053No Hit
TAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTA5070.1420386894339464No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5060.14175853422796228No Hit
AACTTGTGTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT5050.14147837902197818No Hit
CCTCACCATACTGCACTCAGGCAAGCAATAATTTGCTGGGGGGAACTAAT4990.13979744778607348No Hit
CTCTATAACTGTTTCTCTTCCAAAACAGAGACAAGAAATGTGAAACCACA4970.13923713737410526No Hit
GCGATGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT4840.13559511969631174No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT4640.12999201557662945No Hit
GAATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4600.128871394752693No Hit
CTACTGTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4520.12663015310482006No Hit
ACTACATGTTCCGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATA4490.12578968748686772No Hit
ACTACTGTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTC4440.12438891145694715No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4370.12242782501505835No Hit
GTGCTGGTGGGAGTGTCTTTTAGCATGCTGATGCATTGTAATTAGCATAC4350.12186751460309012No Hit
CATCATACAGAAAATTAGAGAATGTTTTAGGAAACTTCCTGTAAACAGAC4300.12046673857316953No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATTAGGGTTAAAGA4260.11934611774923308No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG4250.11906596254324896No Hit
CTCCACAACTTTCCACCAAACTCTGCAAGATCCCAGGGTGAGAGGCCTGT4250.11906596254324896No Hit
ATACCACATCATCCATATAACTAAAAGCCAAACAGTGGGGGAAAGCCCTA4240.11878580733726485No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4240.11878580733726485No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4220.1182254969252966No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4220.1182254969252966No Hit
ATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGTGG4210.1179453417193125No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4200.1176651865133284No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4180.11710487610136015No Hit
GTATAGTAGAAGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAA4160.11654456568939192No Hit
CTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAAC4140.11598425527742369No Hit
CATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGTAAATATACATCGT4080.11430332404151901No Hit
ATCTTATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACC4070.11402316883553489No Hit
ACTATACCTGTCTTTAACCCTAATTGGAAAACACCCTCTTTTCCTAATAT4040.11318270321758253No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4030.11290254801159842No Hit
TTGTAGGTCTTTTGGGTTTTGCTGCTCCTTTTACACAATGTGGTTATCCT4020.11262239280561431No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4020.11262239280561431No Hit
GCGAAGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT4000.11206208239364608No Hit
GTTGAGGAGTTCCACTGCATGGCCTGAGGATGAGTGTCTCTCAAAGGTGG3990.11178192718766197No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT3980.11150177198167785No Hit
GGTATAGTAGAAGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCA3830.10729944389191613No Hit
TCTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG3800.10645897827396378No Hit
CCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG3770.10561851265601142No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAA3750.1050582022440432No Hit
CTTTATGAGTCCAAGGAATACTAACATTGGGGTTCCCGAGATTGAGATCT3740.1047780470380591No Hit
GTTGACGATCCTGGAATTAGAGGACAGACGGGCAACATACCTTGATAGTC3720.10421773662609085No Hit
TCACCATACTGCACTCAGGCAAGCAATAATTTGCTGGGGGGAACTAATGA3720.10421773662609085No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG3720.10421773662609085No Hit
ACTAAATGTTCCGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATA3710.10393758142010674No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3710.10393758142010674No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3700.10365742621412262No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCTCTAGATGCTTGATCTTC3700.10365742621412262No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC3640.10197649497821794No Hit
CTCCTCAACTTTCCACCAAACTCTGCAAGATCCCAGGGTGAGAGGCCTGT3640.10197649497821794No Hit
TTCCCCCACTGTTTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGC3630.10169633977223382No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC3610.10113602936026558No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC3600.10085587415428146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)