FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362972
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC17260.4755187727979017No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16950.4669781691149731No Hit
CTCTATGGAAGGCGGGTATATTATATCAGAGAGAAACAACACATAGCGCC14440.3978268296177116No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13410.3694499851228194No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12190.3358385770803257No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG11610.3198593830929108No Hit
ATGTAAGACCTTGGGCAGGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9960.27440133123216115No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC9080.25015703690642804No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8570.23610636633128726No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8240.22701475595913734No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGAATTGGTGGGCGTTC8090.22288220578997828No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7930.21847415227620862No Hit
CTCTATGACTGTTGCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7570.20855603187022692No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7500.2066275084579527No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT7300.20111744156574063No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7140.19670938805197097No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAGAAGGCCTCCGT7050.19422985795047554No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6830.18816878436904225No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGAAT6780.18679126764598922No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6590.18155670409838776No Hit
TACCTTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6460.1779751606184499No Hit
GGTTAGGGACTGCGAATTTTGGCCAAGACACACGGTGGTTCCCCCTAGAA6430.1771486505846181No Hit
CTACTGTACCTGTCTTTAACCCTCACTGGAAAACACCTTCTTTTCCTAAT6120.16860804690168937No Hit
GTCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC6080.16750603352324697No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6030.16612851680019397No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5980.16475100007714094No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAGAACCTAGCAGG5960.16419999338791974No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5940.16364898669869854No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT5930.1633734833540879No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGAAGTTGGGTCGTC5860.16144495994181368No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG5820.16034294656337128No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5780.15924093318492885No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5770.15896542984031825No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5690.15676140308343345No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5680.15648589973882285No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5610.15455737632654862No Hit
GTCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC5560.1531798596034956No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5390.14849630274511533No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5320.1465677793328411No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTTTGTGTAAACAATACC5280.1454657659543987No Hit
AGTCTGTACAGCATCTTGAGGCCCTTTTTACCGCTGTTACCAATTTTCTT5250.14463925592056687No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGCCGCAAATTGCACC5170.14243522916368204No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAGATACAGGCCTCT5140.14160871912985024No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAGTGAGGGTTAAAGA5100.14050670575140783No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5030.1385781823391336No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5010.1380271756499124No Hit
TACCGTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4900.13499663885919574No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCT4890.13472113551458514No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAGAAC4750.13086408869003668No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA4710.12976207531159428No Hit
CCTTTGGATAGAACCTAGCAGGCATAATTAATTGCAGTCGTCTTTTCTCA4660.12838455858854128No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4600.12673153852087765No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4590.12645603517626705No Hit
CCCCAGAAGGCCTCCGTGCGGTGGGGTGAATCCCGGCCCGAATGCTCCAG4570.12590502848704582No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4550.12535402179782462No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4530.12480301510860341No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4510.1242520084193822No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4500.1239765050747716No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4440.12232348500710799No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT4400.12122147162866558No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4340.11956845156100196No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4320.11901744487178074No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4300.11846643818255954No Hit
TCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGAC4280.11791543149333834No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC4270.11763992814872773No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4270.11763992814872773No Hit
TGGATACGATGTATATTTGCGGGAGAGAACAACAGAGTTATCTGTCCCGA4250.11708892145950653No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT4220.1162624114256747No Hit
CCATATTCTTGGGAACAAGAGCTACGGCATGGGGCAGAATCTTTCCACCA4170.11488489470262168No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4140.11405838466878987No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4120.11350737797956867No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4120.11350737797956867No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4100.11295637129034747No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4050.11157885456729445No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG4010.11047684118885204No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4000.11020133784424144No Hit
ATTTATGCCTACAGCCTCCTAATACAAAAATCATTAACCTAGTCTCCTCC3990.10992583449963084No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3970.10937482781040962No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3960.10909932446579902No Hit
CCTTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT3960.10909932446579902No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG3960.10909932446579902No Hit
CCTCACCATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGACTTAAT3920.1079973110873566No Hit
GTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA3920.1079973110873566No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC3890.10717080105352478No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3860.10634429101969298No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGCCGCAAATTGC3850.10606878767508236No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3850.10606878767508236No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3840.10579328433047176No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTTTGTGTA3820.10524227764125056No Hit
ATCCCAGAGTGAGAGGCCTGTATCTCCCTGCTGGTGGCTCCAGTTCAGGA3800.10469127095202935No Hit
ATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGACTTAATGTCACTG3780.10414026426280815No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA3720.10248724419514453No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3640.1002832174382597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)