FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362972
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC17460.4810288396901138No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16410.4521009885060005No Hit
CTCTATGGAAGGCGGGTATATTATATCAGAGAGAAACAACACATAGCGCC15500.42703018414643557No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG14680.40443890988836606No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13460.37082750184587243No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12310.33914461721565303No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC9210.2537385803863659No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9150.2520855603187023No Hit
CTCTATGACTGTTGCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8970.2471265001157114No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8330.2294942860606328No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8310.22894327937141157No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG8250.22729025930374797No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAGAAGGCCTCCGT7970.21957616565465105No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7590.20910703855944812No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7260.2000154281872982No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7130.19643388470736034No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAGAACCTAGCAGG7020.1934033479166437No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGAATTGGTGGGCGTTC6600.18183220744299836No Hit
CTACTGTACCTGTCTTTAACCCTCACTGGAAAACACCTTCTTTTCCTAAT6510.17935267734150293No Hit
GGTTAGGGACTGCGAATTTTGGCCAAGACACACGGTGGTTCCCCCTAGAA6250.17218959038162723No Hit
ATGTAAGACCTTGGGCAGGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6160.1697100602801318No Hit
TCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGAC6070.16723053017863637No Hit
GTCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC6000.16530200676636214No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT5980.16475100007714094No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5840.16089395325259248No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5790.15951643652953948No Hit
AGTCTGTACAGCATCTTGAGGCCCTTTTTACCGCTGTTACCAATTTTCTT5720.15758791311726525No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5710.15731240977265465No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5590.1540063696373274No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGCCGCAAATTGCACC5540.15262885291427436No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGAAGTTGGGTCGTC5510.15180234288044256No Hit
GTGTAAATGTATATTAGGAAAAGAAGGTGTTTTCCAGTGAGGGTTAAAGA5490.15125133619122136No Hit
TCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGAC5370.14794529605589413No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT5300.1460167726436199No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5170.14243522916368204No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAGAAC5160.14215972581907144No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGAAT5130.14133321578523964No Hit
GTCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC5070.139680195717576No Hit
CCCCAGAAGGCCTCCGTGCGGTGGGGTGAATCCCGGCCCGAATGCTCCAG5070.139680195717576No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4980.13720066561608057No Hit
TACCTTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4980.13720066561608057No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4970.13692516227146997No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4940.13609865223763817No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4920.13554764554841697No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAGATACAGGCCTCT4900.13499663885919574No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4850.13361912213614272No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTTTGTGTA4840.13334361879153211No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4820.13279261210231091No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4800.1322416054130897No Hit
CCTTTGGATAGAACCTAGCAGGCATAATTAATTGCAGTCGTCTTTTCTCA4740.13058858534542608No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA4740.13058858534542608No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4740.13058858534542608No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4730.13031308200081548No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCT4700.12948657196698368No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4670.12866006193315188No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4640.12783355189932005No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4610.12700704186548825No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4600.12673153852087765No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4600.12673153852087765No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC4590.12645603517626705No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4590.12645603517626705No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4560.12562952514243522No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG4500.1239765050747716No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4490.12370100173016099No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTTTGTGTAAACAATACC4470.12314999504093979No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4400.12122147162866558No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4370.12039496159483376No Hit
ATCCCAGAGTGAGAGGCCTGTATCTCCCTGCTGGTGGCTCCAGTTCAGGA4340.11956845156100196No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT4300.11846643818255954No Hit
GTCCCTAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG4230.1165379147702853No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4230.1165379147702853No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4220.1162624114256747No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4200.1157114047364535No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGCCGCAAATTGC4160.11460939135801108No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4070.11212986125651565No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4060.11185435791190505No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4020.11075234453346264No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4010.11047684118885204No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT3990.10992583449963084No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3990.10992583449963084No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGGCCCTTTTTACC3970.10937482781040962No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC3920.1079973110873566No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3910.107721807742746No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3910.107721807742746No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3840.10579328433047176No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAGTCTAAGCAGGCTTTCACTTT3820.10524227764125056No Hit
TCTCGGGGCCGCTTGGGACTTTCTCGTCCCCTTCTCCGTCTGCCGTTTCG3820.10524227764125056No Hit
GTTTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG3790.10441576760741875No Hit
GTACCAAACCTTCGGCCGCAAATTGCACCTGTATTCCCATCCCATCATCC3760.10358925757358695No Hit
GTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA3700.10193623750592333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)