FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455901
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA26030.5709572911662839No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG22430.4919927791340664No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA21640.4746644556603298No Hit
CTCTATGGAAGGCAGGTGTATTATATAAGAGAGAAACAACACATAGCGCC18730.41083480843428727No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAATCT17100.37508143215303325No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14330.314322627061577No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13330.292388040385961No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG11710.25685400997146307No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA10570.2318485811612609No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT10500.23031316009396777No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC9870.2164943704883297No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT9710.21298483662023113No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC9030.1980693176808123No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8980.1969725883470315No Hit
GTACGACATCTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGA8930.19587585901325066No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8770.19236632514515212No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8700.19083090407785902No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8640.18951482887732204No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8520.18688267847624815No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT8510.18666333260949197No Hit
GTATCGGGAAGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTG8370.18359249047490575No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC8330.1827151070078811No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA8190.17964426487329485No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8170.17920557313978255No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8170.17920557313978255No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8160.17898622727302638No Hit
GTATGGATCGGCATAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC8040.17635407687195248No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7950.17437996407114703No Hit
ATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGG7930.1739412723376347No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7850.17218650540358543No Hit
CTCTTATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7760.17021239260277998No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7620.16714155046819376No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7610.16692220460143758No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG7600.16670285873468144No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC7590.16648351286792526No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT7550.16560612940090064No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7500.16450940006711984No Hit
GTATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACC7460.16363201660009521No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT7360.16143855793253362No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7310.16034182859875282No Hit
TGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA7300.16012248273199664No Hit
ATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7280.15968379099848432No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA7250.15902575339821584No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC7210.1581483699311912No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7190.15770967819767887No Hit
ACGACATCTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAAT7130.15639360299714192No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6900.15134864806175025No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT6900.15134864806175025No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6830.14981322699445715No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA6800.14915518939418865No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6780.14871649766067635No Hit
CATGTATTCAGTCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6760.148277805927164No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6740.1478391141936517No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6710.1471810765933832No Hit
GCATAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAG6630.14542630965933392No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6570.14411023445879698No Hit
GTCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA6570.14411023445879698No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6550.14367154272528465No Hit
GTGAAAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA6520.14301350512501618No Hit
TCACCATACTGCTCTCAGGCAAGCAATTCTGTGCTGGGGGGAACTAATGA6440.1412587381909669No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAATCTCCTGA6430.14103939232421073No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6350.13928462539016145No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6330.13884593365664913No Hit
ATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGC6280.13774920432286833No Hit
GAGTTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT6270.13752985845611218No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT6260.13731051258935603No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6250.13709116672259986No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTCTGATGTGAT6150.13489770805503826No Hit
CTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA6090.1335816328545013No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA6060.13292359525423283No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAGTCTAAGC6010.13182686592045204No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5980.13116882832018356No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5870.1287560237858658No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC5840.1280979861855973No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5770.1265625651183042No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5760.12634321925154804No Hit
CTCCTATGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGC5710.12524648991776724No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAGTCTAAGCAGGCTTTTACTTT5620.1232723771169618No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5620.1232723771169618No Hit
GTATGCATGTATTCAGTCTAAGCAGGCTTTTACTTTCTCGCCAACTTACA5600.12283368538344949No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5570.122175647783181No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5530.12129826431615635No Hit
GCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTGCTCTCAGGCA5510.12085957258264404No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5500.1206402267158879No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5490.1204208808491317No Hit
GAGCACACATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATAT5460.11976284324886324No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5440.11932415151535093No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5390.11822742218157013No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5330.11691134698103316No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC5310.11647265524752085No Hit
CCTAATAATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACA5270.1155952717804962No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5230.11471788831347157No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC5180.11362115897969077No Hit
GGGTTACTCTTTACATTTCATGGGTTATGTCATTGGATGTTATGGGTCAT5150.11296312137942228No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5120.1123050837791538No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG5100.11186639204564149No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5090.11164704617888532No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG4920.10791816644403061No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4910.10769882057727447No Hit
CTATGGAAGGCAGGTGTATTATATAAGAGAGAAACAACACATAGCGCCTC4900.10747947471051829No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA4900.10747947471051829No Hit
CTCCGAAAGACACCAAATACTCTATAACTGTTTCTCTTCCAAAAGTGAGA4850.10638274537673749No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4820.10572470777646902No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4780.10484732430944438No Hit
GTTATGGGTCATTGCCACAAGATCACATCAGACAGAAAATCAAAGAATGT4770.10462797844268822No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4770.10462797844268822No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4760.10440863257593207No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4760.10440863257593207No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4730.10375059497566358No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT4720.10353124910890742No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG4690.10287321150863893No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4690.10287321150863893No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4680.10265386564188278No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT4650.1019958280416143No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC4640.10177648217485813No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4630.10155713630810197No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4600.1008990987078335No Hit
CTGTAACTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTAT4600.1008990987078335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)