FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482317
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG37810.783924265576374No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC32100.6655373955303255No Hit
CCTACTAATAATGTCCTCTTGTAAATGAATTTTAGGAAAGGAAGGAGTTT27730.5749330834285334No Hit
CCTATTAACAATGTCCTCTTGTAAATGAATTTTAGGAAAGGAAGGAGTTT24540.5087940089194452No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC20940.43415430100950203No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTGTCAACACTTCCGGA18620.3860531559119832No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCGTG17370.36013659066547515No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17030.353087284918425No Hit
ATTAGTAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG15830.32820738228177737No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14530.30125415442540904No Hit
GTACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGCAACTCTATG12950.26849561595382293No Hit
ATTAACAATGTCCTCTTGTAAATGAATTTTAGGAAAGGAAGGAGTTTGCC12650.26227564029466094No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12610.2614463102067727No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT11970.2481770288005606No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCCTTTTGTGGGTCACCATA11890.24651836862478413No Hit
TTATCTACATACTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCA11770.24403037836111932No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC11650.24154238809745457No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT11610.24071305800956633No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA11110.23034643191096313No Hit
CTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGT10710.22205313103208055No Hit
CTCCAAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG9810.20339320405459482No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG9490.19675856335148878No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG9440.19572190074162843No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCCTTTTGTGGGTCACCATATT9330.19344124299993573No Hit
CTCTTGTACATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC9150.18970925760443857No Hit
TGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGGATA9110.18887992751655033No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8950.1855626071649973No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATTTTAGGAAAGGAAGGAGTTT8910.18473327707710904No Hit
GCACAACTCCTGCTCAAGGCAACTCTATGTTTCCCTCATGTTGCTGTACA8770.18183062176950013No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT8630.17892796646189124No Hit
CCGATGGGCGTTCACGGTGGTCTCCATGCAACGTGCAGAGGTGAAGCGAA8450.17519598106639409No Hit
GTATAAAGGGCCTCACGATGTTGTACAGACTTGGCCCCCAATACCACATC8450.17519598106639409No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG8160.16918333792920426No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT8080.16752467775342772No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7760.16089003705032168No Hit
ATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGGAT7620.1579873817427128No Hit
GTACACAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT7530.1561213890449642No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT7480.15508472643510388No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC7440.15425539634721563No Hit
CATTATTAGTAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAA7340.152182071127495No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG7120.1476207556441096No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6970.14451076781452862No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6890.14285210763875208No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCGTGAGGCCCTTTATACC6850.14202277755086384No Hit
AGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCCAGTCCT6810.1411934474629756No Hit
GCCTAATCATCTCTTGTACATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6810.1411934474629756No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAA6650.13787612711142258No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6620.13725412954550636No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6600.13683946450156226No Hit
ATACCACATCATCCATATAGCTAAAAGCCAAACAGTGGGGGAAAGCCCTA6590.13663213197959018No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6580.13642479945761812No Hit
GTACAACATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6540.13559546936972988No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA6500.1347661392818416No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT6490.13455880675986956No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC6440.13352214415000924No Hit
GCTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG6440.13352214415000924No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCTCTAA6340.1314488189302886No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG6280.13020482379845622No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6200.12854616362267968No Hit
GTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGC6160.12771683353479144No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT6100.12647283840295906No Hit
TTGCACACCTCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGG6060.1256435083150708No Hit
CTTGTACATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT6060.1256435083150708No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA6050.12543617579309874No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCGTGAGGCC6020.12481417822718253No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC5980.12398484813929428No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG5980.12398484813929428No Hit
GGCTGGAGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAAGTA5980.12398484813929428No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCTCTAAACTTCAT5950.12336285057337809No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5930.12294818552943396No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5910.12253352048548984No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5910.12253352048548984No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC5860.12149685787562951No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA5850.12128952535365745No Hit
GTACATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG5820.12066752778774126No Hit
GTCCTGGGCTTTCGCAAAATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT5710.11838687004604855No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT5690.11797220500210442No Hit
ATTCTCTGCTGGGGGGAATTGATGACTCTAGCTACCTGGGTGGGTAATAA5680.11776487248013237No Hit
CCTCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGAT5630.11672820987027205No Hit
TTCCCCCACTGTTTGGCTTTTAGCTATATGGATGATGTGGTATTGGGGGC5620.11652087734829997No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG5570.11548421473843964No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA5500.11403288708463521No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATTCCTATGGGAG5450.11299622447477489No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5440.11278889195280282No Hit
CTATCAATAATGTCCTCTTGTAAATGAATTTTAGGAAAGGAAGGAGTTTG5430.11258155943083077No Hit
CTTGTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGT5410.11216689438688662No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA5380.11154489682097045No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTAAAAGCCAAA5350.11092289925505425No Hit
CTCCTAGACACCGCCTCAGCTCTGTTTCGCGACGCCTTAGAGTCTCCTGA5340.11071556673308218No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5340.11071556673308218No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG5200.10781291142547328No Hit
GTACTTGAATATTTGGTCTCTTTTGGAGTGTGGATTCGCACTCCTCCAGC5180.10739824638152916No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC5060.1049102561178644No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAATTGC5040.10449559107392026No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT4930.10221493333222756No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGA4930.10221493333222756No Hit
GTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT4920.1020076008102555No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA4920.1020076008102555No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4860.1007636056784231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)