FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122595
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18121.47803744035238No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13901.1338145927647947No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACCAGA6680.544883559688405No Hit
GGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGG6410.5228598229944125No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6270.5114401076716016No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA6200.5057302500101962No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5550.45271014315428854No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5230.42660793670214936No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4820.39316448468534604No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4640.3784819935560178No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4390.35808964476528404No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC4270.3483013173457319No Hit
GGCTTTCACTTTCTCACCAACTTACAAGGCCTTTCTGTGTAAACAATACC4140.33769729597455034No Hit
GAATACAAGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGC3950.3221991108935927No Hit
CAAAAAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGG3850.31404217137729923No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3780.3083323137158938No Hit
TACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3710.30262245605448834No Hit
ATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCG3570.29120274073167746No Hit
GGGTTCACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3400.27733594355397856No Hit
AGATTGAATACATGCGTATAAAGGCATTAAAGCAGGATAACCACATTGTG3370.2748888616990905No Hit
ATGCCATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAAT3350.2732574737958318No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA3310.2699946979893144No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCA3270.266731922182797No Hit
CCATAGCATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCACCT3260.2659162282311677No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG3230.2634691463762796No Hit
GTAGAGTTTCTTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCT3190.2602063705697622No Hit
GAGTTTCTTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGT3180.25939067661813286No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC2930.23899832782739916No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT2890.23573555202088176No Hit
ATTCACTCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCCTTTCT2860.23328847016599372No Hit
GTGTCTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCTTCCTGACTGGCG2600.21208042742363065No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA2560.20881765161711324No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC2560.20881765161711324No Hit
GGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCTCACTTT2540.20718626371385457No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG2510.20473918185896653No Hit
CTTCTACTGTTCCGGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT2500.2039234879073372No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT2460.2006607121008198No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2440.19902932419756109No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC2400.19576654839104368No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT2390.19495085443941432No Hit
AGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAA2360.19250377258452628No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA2350.19168807863289694No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG2350.19168807863289694No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG2310.18842530282637954No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACGACAGAGT2250.18353113911660346No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC2220.1810840572617154No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG2190.17863697540682735No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG2170.17700558750356868No Hit
GAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAAT2130.17374281169705127No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT2110.17211142379379257No Hit
ATACATAGAGGTTCTTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCG2110.17211142379379257No Hit
CCCATAGCATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCACC2040.16640156613238713No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2040.16640156613238713No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA1960.15987601451935232No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA1950.15906032056772298No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1930.15742893266446428No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT1900.15498185080957624No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG1840.15008768709980017No Hit
GATTGAATACATGCGTATAAAGGCATTAAAGCAGGATAACCACATTGTGT1820.14845629919654146No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA1810.1476406052449121No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1790.1460092173416534No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC1780.14519352339002406No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT1770.1443778294383947No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT1770.1443778294383947No Hit
TATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCG1760.14356213548676536No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC1710.13948366572861862No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG1700.13866797177698928No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC1680.13703658387373058No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG1670.13622088992210124No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG1650.13458950201884254No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT1650.13458950201884254No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGTGA1620.13214242016395447No Hit
GAATACATGCGTATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA1580.1288796443574371No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTGGTTAATCATTACT1580.1288796443574371No Hit
TCTACGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCAC1570.12806395040580776No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT1560.1272482564541784No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC1560.1272482564541784No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT1550.12643256250254906No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC1490.12153839879277294No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT1460.11909131693788491No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC1460.11909131693788491No Hit
CTACGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC1440.11745992903462621No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA1440.11745992903462621No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT1420.11582854113136752No Hit
GTATTAAACCTTATTATCCAGAACATCTGGTTAATCATTACTTCCAAACC1420.11582854113136752No Hit
GTGTTCACATAACTGACTACTAGGTCCCTGGATATTGGATCTTCCAAATT1410.11501284717973816No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT1410.11501284717973816No Hit
ATCCAGATTGGGACTTCAATCCCAACAAGGACACCTGGCCAGACGCCAAC1400.11419715322810882No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT1400.11419715322810882No Hit
AATCCGGATTTGCGGTGTTTGCTTTGAAGGCTGGATCCAACTGGTGGTCG1380.11256576532485013No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATACGCATGTATT1380.11256576532485013No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA1370.11175007137322077No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAA1350.11011868346996206No Hit
GTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGACCACCAAATG1350.11011868346996206No Hit
ATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCAC1350.11011868346996206No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC1340.10930298951833271No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG1340.10930298951833271No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA1320.10767160161507401No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT1320.10767160161507401No Hit
ACACATAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAAGAGCT1290.10522451976018599No Hit
ATCGAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC1290.10522451976018599No Hit
GTCTGTCAACGTATTGTGGGTCTTTTGGGTTTTGCTGCCCCTTTTACACA1290.10522451976018599No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC1280.10440882580855662No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC1280.10440882580855662No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT1280.10440882580855662No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC1280.10440882580855662No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG1270.10359313185692728No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA1260.10277743790529793No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT1250.1019617439536686No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT1250.1019617439536686No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC1250.1019617439536686No Hit
CATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATT1230.1003303560504099No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC1230.1003303560504099No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT1230.1003303560504099No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA1230.1003303560504099No Hit
GTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGT1230.1003303560504099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)