FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122595
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19911.6240466576940331No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10740.8760553040499206No Hit
GAATACAAGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGC7510.6125861576736409No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6690.5456992536400342No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA6590.5375423141237408No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACCAGA6360.5187813532362657No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5290.4315021004119254No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG5230.42660793670214936No Hit
GGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGG4720.38500754516905256No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC4560.371956441942983No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC4440.3621681145234308No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4360.35564256291039603No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4160.33932868387780907No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4120.33606590807129166No Hit
GGCTTTCACTTTCTCACCAACTTACAAGGCCTTTCTGTGTAAACAATACC3930.32056772299033404No Hit
GGGTTCACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3490.2846771891186427No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3460.28223010726375464No Hit
CAAAAAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGG3440.28059871936049596No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG3330.27162608589257314No Hit
ATGCCATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAAT3300.2691790040376851No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3230.2634691463762796No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG3050.24878665524695134No Hit
CCATAGCATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCACCT3010.24552387944043394No Hit
ATTCACTCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCCTTTCT2810.22921000040784698No Hit
GGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCTCACTTT2760.22513153064970026No Hit
AGATTGAATACATGCGTATAAAGGCATTAAAGCAGGATAACCACATTGTG2760.22513153064970026No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC2750.22431583669807087No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG2740.22350014274644153No Hit
GAGTTTCTTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGT2740.22350014274644153No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2740.22350014274644153No Hit
TACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2570.20963334556874258No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA2560.20881765161711324No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC2530.2063705697622252No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCA2500.2039234879073372No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC2400.19576654839104368No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT2400.19576654839104368No Hit
GTGTCTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCTTCCTGACTGGCG2390.19495085443941432No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG2380.19413516048778498No Hit
ATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCG2340.1908723846812676No Hit
GTAGAGTTTCTTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCT2320.1892409967780089No Hit
GAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAAT2270.18516252701986216No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG2270.18516252701986216No Hit
CTTCTACTGTTCCGGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT2230.18189975121334476No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC2160.17618989355193931No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT2130.17374281169705127No Hit
AGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAA2120.1729271177454219No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC2120.1729271177454219No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA2100.17129572984216324No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA2060.16803295403564583No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT2050.16721726008401647No Hit
CCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAA2050.16721726008401647No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA1970.1606917084709817No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1940.15824462661609362No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT1900.15498185080957624No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG1870.1525347689546882No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACGACAGAGT1860.15171907500305884No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG1840.15008768709980017No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTGGTTAATCATTACT1840.15008768709980017No Hit
GATTGAATACATGCGTATAAAGGCATTAAAGCAGGATAACCACATTGTGT1820.14845629919654146No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC1810.1476406052449121No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT1800.14682491129328276No Hit
CCCATAGCATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCACC1790.1460092173416534No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC1760.14356213548676536No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG1720.14029935968024798No Hit
GTGTTCACATAACTGACTACTAGGTCCCTGGATATTGGATCTTCCAAATT1710.13948366572861862No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1710.13948366572861862No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT1690.13785227782535991No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA1650.13458950201884254No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC1630.13295811411558384No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG1620.13214242016395447No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGTGA1620.13214242016395447No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA1580.1288796443574371No Hit
TATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCG1560.1272482564541784No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT1560.1272482564541784No Hit
TCTACGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCAC1510.12316978669603164No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG1480.1207227048411436No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG1470.11990701088951425No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC1460.11909131693788491No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA1440.11745992903462621No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA1430.11664423508299686No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG1410.11501284717973816No Hit
ATACATAGAGGTTCTTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCG1400.11419715322810882No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC1380.11256576532485013No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT1360.1109343774215914No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT1340.10930298951833271No Hit
GATCTTGTGGCAATGACCCATAGCATCCAATGACATAGCCCATGAAATGT1340.10930298951833271No Hit
ATCCAGATTGGGACTTCAATCCCAACAAGGACACCTGGCCAGACGCCAAC1320.10767160161507401No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT1320.10767160161507401No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT1310.10685590766344467No Hit
GTATTAAACCTTATTATCCAGAACATCTGGTTAATCATTACTTCCAAACC1300.10604021371181532No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA1290.10522451976018599No Hit
ATGTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTA1290.10522451976018599No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT1280.10440882580855662No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG1280.10440882580855662No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT1270.10359313185692728No Hit
GTTTGGTGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTT1270.10359313185692728No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATACGCATGTATT1270.10359313185692728No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT1270.10359313185692728No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC1260.10277743790529793No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT1260.10277743790529793No Hit
GTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGT1250.1019617439536686No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT1250.1019617439536686No Hit
AATCCGGATTTGCGGTGTTTGCTTTGAAGGCTGGATCCAACTGGTGGTCG1250.1019617439536686No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC1240.10114605000203923No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT1240.10114605000203923No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA1240.10114605000203923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)