FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381737
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20420.5349232586833343No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16920.4432370977924592No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG16450.43092495618711313No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12950.33923879529623796No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA11310.29627727990737074No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11310.29627727990737074No Hit
CTACTGTGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGT10780.2823933755438954No Hit
TTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGACTGCAATTGATTATG10660.2792498500276368No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10420.27296279899511966No Hit
ATCCCAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9900.25934085509133253No Hit
CTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGT9340.2446710693487925No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT8740.22895344176749963No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC8590.22502403487217637No Hit
GGCCCACAAATTGTTCACATTTTTTGATAATATCTTGGTGTAAATGTATA8580.22476207441248816No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG8530.2234522721140471No Hit
ATCCGAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8460.2216185488962296No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7940.20799660499244244No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7750.20301935625836637No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC7620.19961387028241956No Hit
CATTGGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA7540.19751818660491385No Hit
CCCTCACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTATGGGCTATG7520.1969942656855374No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7330.19201701695146134No Hit
ACACATAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAAGAGCT7220.189135451894891No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7160.18756368913676172No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6930.18153859856393276No Hit
CATTCGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA6920.18127663810424455No Hit
GTACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6890.1804907567251799No Hit
GTACAGACTTGGCCCCCAGTACCACATCATCCATATAACTGAAAGCCAAA6820.1786570335073624No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC6800.17813311258798598No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6790.17787115212829777No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6650.17420370569266275No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCGAGTACAAAGACC6590.17263194293453346No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6470.16948841741827486No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6440.16870253603921023No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA6410.16791665466014558No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6400.1676546942004574No Hit
GGATGTTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA6320.16555901052295166No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6110.16005784086949915No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGAGTTAAAGA5760.15088922478041164No Hit
CTCAAGATGCTGTACAGACTTGGCCCCCAGTACCACATCATCCATATAAC5730.150103343401347No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5720.14984138294165877No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC5700.14931746202228235No Hit
CTTCTACTGTTCCTGTCTTTAACTCTCATTGGAAAACACCCTCTTTTCCT5660.1482696201835295No Hit
TCCAACATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCC5530.1448641342075827No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5510.1443402132882063No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5500.14407825282851805No Hit
TTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC5420.14198256915101234No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5380.14093472731225948No Hit
GGAAAATACAGGCCTTTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT5350.14014884593319482No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC5320.1393629645541302No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5320.1393629645541302No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5280.13831512271537735No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA5250.1375292413363127No Hit
GTATCTTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCA5170.13543355765880696No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5150.13490963673943054No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4990.1307182693844191No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4900.1283606252472252No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4850.12705082294878411No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4830.1265269020294077No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4810.12600298111003125No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGAGGGCC4780.1252170997309666No Hit
CAAGATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT4770.1249551392712784No Hit
CACTACTGTGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4750.12443121835190196No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4750.12443121835190196No Hit
GTCATGGGCCCTCAGCGCATGCGTGGAACCTTTCGGGCTCCTCTGCCGAT4740.12416925789221374No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4740.12416925789221374No Hit
CTCTACTTCCAGGATCTTCAACCACCAGCACGGGACCATGCAGAACCTGC4710.12338337651314911No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4700.12312141605346089No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA4670.12233553467439626No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4650.12181161375501982No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4590.12023985099689054No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG4580.11997789053720231No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4570.1197159300775141No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4570.1197159300775141No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4540.11893004869844945No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC4540.11893004869844945No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG4530.11866808823876125No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4510.11814416731938482No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4480.11735828594032016No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4420.11578652318219088No Hit
ATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACCTG4390.11500064180312625No Hit
GGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTC4300.1126429976659323No Hit
TCATAGGCCCTCAGCGCATGCGTGGAACCTTTCGGGCTCCTCTGCCGATC4270.11185711628686765No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4260.11159515582717945No Hit
GTGAAAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA4240.11107123490780302No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC4210.11028535352873836No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4170.10923751168998551No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4130.10818966985123264No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4040.10583202571403873No Hit
GTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGAC3990.10452222341559765No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3980.10426026295590944No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3980.10426026295590944No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG3960.10373634203653301No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA3900.10216457927840372No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3880.1016406583590273No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG3880.1016406583590273No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT3870.10137869789933907No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3840.10059281652027444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)