FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503708
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC20910.41512145925814165No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG19230.38176880256021345No Hit
ATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC18860.37442327697793165No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC16730.3321368729502013No Hit
AATTTGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT15630.310298823921796No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA15620.3101002962033559No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14610.290048996640911No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA14340.2846887482430297No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13610.27019622479690614No Hit
TCAAAGACCACTGGCCAGCAGCCAACCAGGTAGGAGTGGGAGCATTCGGG13550.2690050584862659No Hit
GAGGTCATCCACACGTAGCGCATCATTTTGCGGGTCACCATATTCTTGGG13020.25848308940894327No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA12100.24021853931245882No Hit
CCTCCACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12070.2396229561571387No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA11890.23604945722521778No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT10580.2100423261095714No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG9960.1977336075662884No Hit
GGTCCTTGATGGGGTTGAAGTCCCAATCTGGATTGTTTGAGTTGGCTCCG9390.18641752761520564No Hit
CCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT9370.18602047217832554No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC9130.18125580693576437No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC9100.18066022378044422No Hit
GGTGATCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG8910.1768881971300833No Hit
CTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGG8810.17490291994568283No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT8760.17391028135348258No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT8530.16934414382936144No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG8410.16696181120808087No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8360.16596917261588062No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT8230.16338831227616No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT7780.1544545649463578No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGCGCTTGGTGGAAGGC7480.14849873339315636No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7450.1479031502378362No Hit
AGTTGGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT7430.1475060948009561No Hit
GCTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG7430.1475060948009561No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCTAGCTGTGC7190.14274142955839494No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7140.1417487909661947No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT6860.13619001484987334No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG6770.13440326538391292No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6750.1340062099470328No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6750.1340062099470328No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG6640.13182240504419226No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC6610.13122682188887214No Hit
CCTCAAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT6490.12884448926759154No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT6490.12884448926759154No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6490.12884448926759154No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA6190.12288865771439009No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG6100.12110190824842966No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTTA5970.11852104790870902No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG5940.11792546475338887No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5930.11772693703494881No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC5850.11613871528742842No Hit
CATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACC5830.11574165985054834No Hit
GAGGAAATCCACACGTAGCGCATCATTTTGCGGGTCACCATATTCTTGGG5820.11554313213210828No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC5820.11554313213210828No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG5820.11554313213210828No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5810.11534460441366824No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5800.11514607669522818No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTGCAAATT5760.114351965821468No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA5700.1131607995108277No Hit
CTATAGGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTC5690.11296227179238764No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5690.11296227179238764No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT5640.11196963320018741No Hit
GGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA5640.11196963320018741No Hit
AGTGTGGAGTTACTCTCGTTTTTGCCTCATGACTTCTTTCCTTCCGTCAG5620.11157257776330731No Hit
AGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCCAGTCCT5620.11157257776330731No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5600.11117552232642722No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5560.11038141145266703No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5550.11018288373422698No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5550.11018288373422698No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA5520.10958730057890682No Hit
CTCTTATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTC5520.10958730057890682No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5520.10958730057890682No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5490.10899171742358668No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5440.10799907883138643No Hit
CTATATAAGAGGTCATCCACACGTAGCGCATCATTTTGCGGGTCACCATA5440.10799907883138643No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT5440.10799907883138643No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5410.10740349567606629No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCACC5360.10641085708386605No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC5340.10601380164698596No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCACCCTATAGACCACCAAATGCC5330.1058152739285459No Hit
ACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5300.10521969077322577No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG5290.1050211630547857No Hit
GTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5260.10442557989946556No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG5240.10402852446258547No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT5240.10402852446258547No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA5230.1038299967441454No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT5190.10303588587038523No Hit
CTCCAAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5170.10263883043350513No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5150.10224177499662503No Hit
GGAAGATACAGACCCCTGACTCTGGGATCCTGCAGCGCTTGGTGGAAGGC5130.10184471955974493No Hit
ACCCTCTGGGATTCTTTCCCGATCATCAGTTGGACCCTGCATTCGGAGCC5130.10184471955974493No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5100.10124913640442477No Hit
CCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT5090.10105060868598474No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT5080.10085208096754468No Hit
GGAAAATACAGACCCCTGATTCTGGGATCCTGCAGCGCTTGGTGGAAGGC5060.1004550255306646No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5040.1000579700937845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)