FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503708
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC18160.36052633668712825No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG17690.351195533920446No Hit
ATATAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC15010.29799010537851295No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA14870.29521071732035226No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA14570.2892548857671508No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13100.26007131115646365No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC12980.2576889785351831No Hit
AATTTGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT12940.2568948676614229No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12250.24319645508905952No Hit
CCTCCACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12080.23982148387557872No Hit
TCAAAGACCACTGGCCAGCAGCCAACCAGGTAGGAGTGGGAGCATTCGGG11910.2364465126620979No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA11850.2352553463514576No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT11320.22473337727413503No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10240.2032923836826098No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG10190.20229974509040952No Hit
GAGGTCATCCACACGTAGCGCATCATTTTGCGGGTCACCATATTCTTGGG10110.20071152334288916No Hit
GGTCCTTGATGGGGTTGAAGTCCCAATCTGGATTGTTTGAGTTGGCTCCG8750.17371175363504252No Hit
CCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT8720.1731161704797224No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC8240.16358683999460005No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT8230.16338831227616No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT8200.16279272912083984No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC8190.1625942014023998No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG8170.1621971459655197No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT8060.16001334106267917No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG8040.15961628562579908No Hit
CTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGG7870.15624131441231826No Hit
GGTGATCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG7690.15266781548039737No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7410.14710903936407602No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7030.13956498606335418No Hit
CCTCAAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT6880.13658707028675343No Hit
AGTTGGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT6800.13499884853923305No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCTAGCTGTGC6680.13261651591795245No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6650.13202093276263233No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG6640.13182240504419226No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6570.13043271101511192No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT6500.1290430169860316No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG6420.1274547952385112No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6400.1270577398016311No Hit
GCTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG6370.12646215664631094No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6350.12606510120943087No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT6320.12546951805411072No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG6320.12546951805411072No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCACCCTATAGACCACCAAATGCC6090.1209033805299896No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT6060.12030779737466944No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5910.11732988159806873No Hit
GGAAAATACAGACCCCTGACTCTGGGATCCTGCAGCGCTTGGTGGAAGGC5900.11713135387962867No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5870.11653577072430851No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5850.11613871528742842No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5830.11574165985054834No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT5820.11554313213210828No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA5800.11514607669522818No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTGCAAATT5770.11455049353990804No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC5730.11375638266614785No Hit
CTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTG5660.11236668863706752No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5640.11196963320018741No Hit
CTATAGGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTC5610.11137405004486728No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA5600.11117552232642722No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5600.11117552232642722No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCACC5580.11077846688954712No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG5540.10998435601578692No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC5530.10978582829734689No Hit
ACCCTCTGGGATTCTTTCCCGATCATCAGTTGGACCCTGCATTCGGAGCC5480.10879318970514665No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG5480.10879318970514665No Hit
CTCCAAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5450.10819760654982649No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5440.10799907883138643No Hit
GGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA5430.1078005511129464No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5420.10760202339450634No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT5410.10740349567606629No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT5360.10641085708386605No Hit
CCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT5290.1050211630547857No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5270.10462410761790562No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA5230.1038299967441454No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT5220.10363146902570537No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTTA5210.10343294130726532No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG5200.10323441358882526No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5150.10224177499662503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)