FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189803
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA36601.9283151478111513No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT14880.7839707486183043No Hit
CATCTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8190.43150002897741346No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC7800.41095240854991755No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC7360.3877704778112042No Hit
GTATAGATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT7030.3703840297571693No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG6850.3609005126367866No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6010.3166440994083339No Hit
GTGCTGGTGAGGTACCATGCTTGGGGGCACGTGGTGGCTGAAGGGAGGGA5560.2929353066073771No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5270.2776563068023161No Hit
GTGCTGGTGTTGGCAGGTGACGAGAATCACAGAGGTGCCGTGGAGAAAGG5220.27502199649109865No Hit
GTCTCGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCGTG5160.27186082411763773No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA4880.2571086863748202No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4690.247098307192194No Hit
GCTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG4450.23445361769835038No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4100.21601344551982846No Hit
GCATCTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGC3940.2075836525239327No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC3900.20547620427495877No Hit
TACTCACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTT3790.19968072159028044No Hit
AGTTTGCAGCACAGCCTAGCAGCCATGGAAACGATGTATATTTTCGCGAG3680.1938852389056021No Hit
CCATATTCGTCACAGGGTCCCCAGTCCTCGCGGAGATTGACGAGATGTGA3590.18914348034541076No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC3580.18861661828316728No Hit
GATTAAAATTAATTATGCCTGCTAGATTCTATCCTAACCACACTAAATAT3560.18756289415868033No Hit
GTCCCAGAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC3480.18334799766073243No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGAAAATATACATCGT3390.1786062391005411No Hit
CGTTCTTGCCTCATGACTTCTTTCCTTCCGTCAGAGATCTCCTAGACACC3370.17755251497605412No Hit
CTTTTTACCGCCGTAACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA3360.17702565291381064No Hit
GGATCCCAGAGTCAGGGGTCTGTATTTTCCTGCTGGTGGCTCCAGTTCAG3340.17597192878932366No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3340.17597192878932366No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA3320.1749182046648367No Hit
ATTTCAATCTTCTCTTTTCATTTACAGTAAGAGGGCCCACAAATTGTTGA3320.1749182046648367No Hit
GTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAA3290.17333761847810625No Hit
GCATTGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC3180.16754213579342792No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA3160.16648841166894096No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT3100.16332723929548004No Hit
GTACAGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG3090.16280037723323657No Hit
TCTCATGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT3070.16174665310874958No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT3010.1585854807352887No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT2940.15489744629958432No Hit
ATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTG2850.15015568773939295No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC2820.1485751015526625No Hit
CTTGCAAATGAATCTTAGGAAAGGAAGGAGTTTGCCATTCAGGATTAAAG2800.14752137742817553No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGCTT2730.14383334299247114No Hit
CCTCTTCATCCTGCTGCTATGCCTCATCTTCTTATTGGTTCTTCTGGATT2720.14330648093022766No Hit
ACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTACG2620.1380378603077928No Hit
AGTCGAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC2600.13698413618330585No Hit
CCTAGAAGAAGAACTCCCTCGCCTCGCAGACGCAGATCTCAATCGCCGCG2590.13645727412106234No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA2400.12644689493843617No Hit
CTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATG2400.12644689493843617No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC2340.12328572256497525No Hit
ACCACATCATCCATATAGCTAAAAGCCAAACAGTGGGGGAAAGCCCTACG2280.12012455019151437No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT2250.11854396400478391No Hit
GGGTAAAGGTTCATGTACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGA2240.11801710194254042No Hit
CGGACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTA2230.11749023988029694No Hit
GGTCTGTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGC2210.11643651575580997No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG2190.115382791631323No Hit
CATGTATACAGGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC2190.115382791631323No Hit
GCTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG2190.115382791631323No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2140.11274848132010558No Hit
ATTCTATCCTAACCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC2090.11011417100888818No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG2060.10853358482215772No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA2060.10853358482215772No Hit
ATTTTAATCTTCTCTTTTCATTTACAGTAAGAGGGCCCACAAATTGTTGA2050.10800672275991423No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC2040.10747986069767075No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT2030.10695299863542726No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG2020.10642613657318378No Hit
GGTAATGACGGAGGAGGCGTCCCTCCACAGGGCCAGGCTCGCCATGGGCC2010.10589927451094029No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG2010.10589927451094029No Hit
GTCCCTTGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATT1970.10379182626196636No Hit
CTCTAAATCTGGGGCCAGCAAGTCACCAGCGACCTCTGGTACTAAGGAGG1970.10379182626196636No Hit
GGGATCTAGTAGTCAATTATGTTAATACTCACATGGGTTTAAAGATCAGG1950.10273810213747939No Hit
CACACAGACTCTGTGTGTGTAAGGAACGCAATCTCAGCACACGCCCACGT1940.1022112400752359No Hit
CCTATTCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACACAGTCTTTGA1930.1016843780129924No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATCTAT1920.10115751595074893No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA1920.10115751595074893No Hit
GTTTTCCATTCAGGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGT1920.10115751595074893No Hit
CCATGTCCGTCACAGGGTCCCCAGTCCTCGCGGAGATTGACGAGATGTGA1920.10115751595074893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)