FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189803
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA33351.7570849775820192No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT14120.7439292318877995No Hit
CATCTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7710.40621064998972617No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG6820.3593199264500561No Hit
GTATAGATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT6660.35089013345416037No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC6590.347202099018456No Hit
GTGCTGGTGAGGTACCATGCTTGGGGGCACGTGGTGGCTGAAGGGAGGGA6090.3208589959062818No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC6030.3176978235328209No Hit
GTCTCGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCGTG5740.30241882372775986No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5690.29978451341654244No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5180.2729145482421247No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA4900.25816241049930716No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4660.24551772100546357No Hit
GTGCTGGTGTTGGCAGGTGACGAGAATCACAGAGGTGCCGTGGAGAAAGG4400.23181930738713297No Hit
CCTCTTCATCCTGCTGCTATGCCTCATCTTCTTATTGGTTCTTCTGGATT4310.22707754882694162No Hit
TACTCACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTT4160.21917461789328938No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4100.21601344551982846No Hit
GCTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG4100.21601344551982846No Hit
GCATTGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC4030.21232541108412406No Hit
CTTTTTACCGCCGTAACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA3920.20652992839944573No Hit
GCATCTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGC3800.20020758365252392No Hit
GGATCCCAGAGTCAGGGGTCTGTATTTTCCTGCTGGTGGCTCCAGTTCAG3610.19019720446989774No Hit
CCATATTCGTCACAGGGTCCCCAGTCCTCGCGGAGATTGACGAGATGTGA3560.18756289415868033No Hit
AGTTTGCAGCACAGCCTAGCAGCCATGGAAACGATGTATATTTTCGCGAG3450.181767411474002No Hit
GTACAGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG3380.1780793770382976No Hit
GATTAAAATTAATTATGCCTGCTAGATTCTATCCTAACCACACTAAATAT3270.17228389435361927No Hit
CGTTCTTGCCTCATGACTTCTTTCCTTCCGTCAGAGATCTCCTAGACACC3200.1685958599179149No Hit
ATTTCAATCTTCTCTTTTCATTTACAGTAAGAGGGCCCACAAATTGTTGA3150.16596154960669748No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA3140.16543468754445398No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC3120.16438096341996702No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3110.16385410135772355No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC3020.15911234279753217No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGAAAATATACATCGT2920.15384372217509734No Hit
TCTCATGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT2880.1517362739261234No Hit
CTCTAAATCTGGGGCCAGCAAGTCACCAGCGACCTCTGGTACTAAGGAGG2830.14910196361490596No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA2800.14752137742817553No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT2780.14646765330368855No Hit
AGTCGAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC2760.1454139291792016No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT2740.14436020505471464No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC2690.1417258947434972No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGCTT2690.1417258947434972No Hit
ATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTG2670.14067217061901024No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC2660.14014530855676674No Hit
ACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTACG2630.1385647223700363No Hit
CTTGCAAATGAATCTTAGGAAAGGAAGGAGTTTGCCATTCAGGATTAAAG2630.1385647223700363No Hit
GGTCTGTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGC2540.13382296380984496No Hit
GGGTAAAGGTTCATGTACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGA2530.13329610174760148No Hit
GCTCATCGGAACTGACAATTCTGTCGTCCTCTCGCGGAAATATACATCGT2520.13276923968535798No Hit
GTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAA2490.13118865349862752No Hit
GTCCCAGAGTCCTCTTATGTAAGACCTTGGGCAGGATCTGATGGGCGTTC2460.12960806731189708No Hit
CTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTTTACGTCCCGTCGGCG2460.12960806731189708No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT2410.12697375700067964No Hit
CCTAGAAGAAGAACTCCCTCGCCTCGCAGACGCAGATCTCAATCGCCGCG2310.12170513637824482No Hit
GTTTTCCATTCAGGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGT2210.11643651575580997No Hit
GTACTAAGGAGGTCAGCCCGGGGGGATGAGTTACAATGAGTTACACAAGC2210.11643651575580997No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2180.11485592956907952No Hit
CATGTATACAGGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC2120.11169475719561861No Hit
GCCTTCCACCAAGCTCTGCAGGATCCCAGAGTCAGGGGTCTGTATTTTCC2100.11064103307113166No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA2100.11064103307113166No Hit
GGGATCTAGTAGTCAATTATGTTAATACTCACATGGGTTTAAAGATCAGG2080.10958730894664469No Hit
ATTTTAATCTTCTCTTTTCATTTACAGTAAGAGGGCCCACAAATTGTTGA2060.10853358482215772No Hit
GAGTTACACAAGCCCGTTCGTGACCCACATCGCCTGCCTGACACACTCCA2020.10642613657318378No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA2010.10589927451094029No Hit
CGGACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTA2010.10589927451094029No Hit
GTCCCTTGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATT1980.10431868832420983No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG1970.10379182626196636No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC1970.10379182626196636No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA1960.10326496419972286No Hit
ACCACATCATCCATATAGCTAAAAGCCAAACAGTGGGGGAAAGCCCTACG1960.10326496419972286No Hit
CTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATG1950.10273810213747939No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA1910.10063065388850544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)