FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511635
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA25250.4935158853479531No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG23130.4520800961623032No Hit
AGCTAGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTAAAA20250.39578996745726935No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18410.3598268296734977No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC17650.34497249015411374No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTCTGGAAGTAATGATTAACTAGA16780.3279681804411348No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15560.30412305647580795No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT14800.289268716956424No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14320.27988702883891836No Hit
GTTTGCATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG13020.2544782901873406No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC12430.24294663187623988No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT11760.22985135887888825No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT11750.2296559070431069No Hit
ATGTAAGACCTTGGGCAAGATCCGGTGGGCGTTCACGGTGGTCTCCATGC11580.22633322583482368No Hit
GTCCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGC11370.22222873728341494No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCATCAAACTCTGCAAG11260.2200787670898199No Hit
ATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGCA11080.2165606340457553No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA10830.2116743381512211No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT10690.20893801245028196No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG10630.20776530143559374No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG10520.20561533124199868No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10420.203660812884185No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT10190.19916542066121357No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTAAAA9900.1934973174235539No Hit
GGAATATGATAAAACGCCGCAGACACATCCAACGATAACCAGGACAAGTT9800.1915427990657402No Hit
GGATAGGACAACAGAGTTATCAGTCCCGAGAATGTTTGCTCCAGACCTGC9220.1802065925904209No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8990.17571120036744944No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC8930.17453848935276123No Hit
GTGTAAATAGTGTCTAGTCTGGAAGTAATGATTAACTAGATGTTCTGGAT8810.17219306732338485No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA8740.17082490447291526No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT8650.16906583795088295No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATCCGGTGGGCGTTC8650.16906583795088295No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT8550.16711131959306927No Hit
GGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTGGAGGC8540.16691586775728792No Hit
TAATACAAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAG8400.16417954205634877No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC8390.1639840902205674No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC8250.16124776451962824No Hit
GCCTGAGGATGCGTGTTTCGCAAAGGTGGAGACAGCGGGGTAGGCTGCCT8200.1602705053407214No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCACTTATCGTCAATCTTCTCG8180.15987960166915866No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8130.15890234249025184No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8090.15812053514712637No Hit
GTGTCTAGTCTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTT8060.15753417963978228No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8030.15694782413243819No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8020.1567523722966568No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT8000.15636146862509406No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7980.15597056495353134No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA7900.15440695026728038No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7850.15342969108837354No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7780.15206152823790398No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA7740.1512797208947785No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT7630.14912975070118345No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT7600.14854339519383936No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAA7560.1477615878507139No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT7510.14678432867180705No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC7470.14600252132868158No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7370.1440480029708679No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA7340.1434616474635238No Hit
GTCTCCACCTTTGCGAAACACGCATCCTCAGGCCATGCAGTGGAACTCCA7190.1405298699268033No Hit
CCCCAAAAGGCCGCCGTGCGGTGGGGTGAAACCCAGCCCGAATGCTCCAG7180.14033441809102193No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC7120.1391617070763337No Hit
CCTTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7040.13759809239008278No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6980.13642538137539456No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6890.13466631485336225No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6700.13095272997351626No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6650.12997547079460942No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6640.12978001895882807No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT6490.12684824142210757No Hit
CTTCTAGATACCGCCTCAGCTTTGTATCGGGATGCCTTAGAGTCTCCTGA6480.1266527895863262No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6390.12489372306429389No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6380.12469827122851251No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6320.12352556021382431No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG6320.12352556021382431No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6320.12352556021382431No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTTGGGTTTT6210.12137559002022927No Hit
GTTTAATACCTTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC6210.12137559002022927No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6180.12078923451288516No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC6130.11981197533397832No Hit
TTCTAAAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6120.11961652349819696No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6070.11863926431929012No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCGGCGAC6020.11766200514038327No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6020.11766200514038327No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6020.11766200514038327No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT5950.1162938422899137No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5940.11609839045413234No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5880.11492567943944412No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCGGCGACGCGGCGA5800.1133620647531932No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5790.11316661291741184No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5650.11043028721647269No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC5610.10964847987334722No Hit
GTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA5590.10925757620178447No Hit
CCGCCGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGA5560.10867122069444038No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGAT5510.10769396151553354No Hit
ATGCGGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5490.1073030578439708No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT5490.1073030578439708No Hit
GTTTACGTCCCCTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGTCG5340.10437128030725028No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5340.10437128030725028No Hit
GTTTGATGGAAGGTTGTGGAGTTCCACTGCATGGCCTGAGGATGCGTGTT5280.10319856929256208No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC5260.10280766562099934No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5230.10222131011365523No Hit
GGATAAAGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5210.1018304064420925No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT5210.1018304064420925No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA5190.10143950277052977No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5130.10026679175584156No Hit
GGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGGTCAT5120.10007133992006019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)