FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368039
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG31030.8431171696477819No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC29130.7914922059890392No Hit
CCTCCAGCTTATAGACCACTAAATGCCCCTATCTTATCAACACTTCCGGA27010.7338896149592842No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA15900.4320194327231625No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG15000.40756550256902124No Hit
ATATCAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT12030.326867533060355No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTGATTCCAGGATCTTCAACCACC12030.326867533060355No Hit
CTCTATGGAAGGCGGGTGTATTATATCAGAGAGAAACAACACGTAGCGCC11230.3051307062566739No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA10680.2901866378291431No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA10360.28149190710767064No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC10180.2766011210768424No Hit
CCTCAAGCTTATAGACCACTAAATGCCCCTATCTTATCAACACTTCCGGA10170.2763294107417964No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9660.26247218365444963No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8930.24263732919609063No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA8770.2382899638353544No Hit
GAGTATTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGG8680.23584457081994026No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA8540.2320406261292961No Hit
CTCTTATAAAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8540.2320406261292961No Hit
ATCAGGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC8460.22986694344892797No Hit
CTCTAATTCCAGGATCTTCAACCACCAGCGCGGGACCATGCAGAACCTGC8060.21899853004708741No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG7930.21546629569148923No Hit
GAATCAGAGGACAGACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC7930.21546629569148923No Hit
GATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGG7850.21329261301112112No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7450.20242419960928054No Hit
ATACTATGCTGGGGGGAACTAATGACTCTAGCTACCTGGGTGGGTGTTAA6990.18992552419716388No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6890.18720842084670375No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6840.18584986917147367No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC6840.18584986917147367No Hit
AGTCTAGACTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6820.18530644850138164No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6800.18476302783128962No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6520.17715513845000122No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6420.17443803509954106No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC6340.17226435241917298No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6320.17172093174908093No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGTCCACT6050.16438475270283856No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6040.16411304236779253No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT6030.16384133203274653No Hit
ATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGACT6030.16384133203274653No Hit
CTCTGATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6020.16356962169770053No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCATCAGTTGG6000.16302620102760848No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG5990.16275449069256248No Hit
GTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCG5820.15813541499678024No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTCGA5760.15650515298650414No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5740.1559617323164121No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA5710.15514660131127408No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC5670.15405975997109No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5610.15242949796081395No Hit
TATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCG5520.1499841049453998No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5500.1494406842753078No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5440.1478104222650317No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5420.14726700159493966No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5360.14563673958466358No Hit
GGACAGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGTTTCCATGG5300.1440064775743875No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT5290.14373476723934148No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5280.14346305690429548No Hit
GTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATAC5240.14237621556411142No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5170.14047424321878932No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG5140.13965911221365127No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5130.13938740187860527No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG5060.13748542953328316No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT5060.13748542953328316No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5020.1363985881930991No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4990.13558345718796105No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4980.13531174685291503No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC4960.13476832618282303No Hit
GTGCTGGTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAG4890.13286635383750092No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4890.13286635383750092No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4870.13232293316740887No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4840.13150780216227084No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC4780.12987754015199476No Hit
ATATCAGAGAGAAACAACACGAAGCGCCTCATTTTGTGGGTCACCATATT4730.12851898847676468No Hit
GAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGG4710.12797556780667269No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT4690.12743214713658063No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC4580.12444333345107447No Hit
CATTTACTGTGAGTGGACCTACAAATTGTTCACATTTTTTGATAATGTCT4550.12362820244593645No Hit
ATACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4520.1228130714407984No Hit
CCTTTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG4500.12226965077070635No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG4490.12199794043566035No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4440.12063938876043029No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4430.12036767842538428No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4370.11873741641510818No Hit
CTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA4360.11846570608006217No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC4330.11765057507492412No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT4330.11765057507492412No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT4310.1171071544048321No Hit
CTTATAAAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4310.1171071544048321No Hit
GTATTATATCAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACC4310.1171071544048321No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT4280.11629202339969405No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4270.11602031306464805No Hit
GAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCTTCGT4160.11303149937914188No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4120.11194465803895783No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4110.11167294770391181No Hit
AGGAATCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCCAATCCT4080.11085781669877377No Hit
GTATGCATGTATTCAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACA4070.11058610636372776No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4020.1092275546884977No Hit
GGTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTT4020.1092275546884977No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAACAGGCTTTCACTTT4010.10895584435345167No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3990.10841242368335964No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC3980.10814071334831364No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3980.10814071334831364No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3980.10814071334831364No Hit
CAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAA3970.1078690030132676No Hit
GACCTGGCCGTTGCCTAGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC3930.10678216167308355No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAAC3920.10651045133803755No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3910.10623874100299154No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3910.10623874100299154No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA3900.10596703066794552No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3890.1056953203328995No Hit
TCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTG3880.10542360999785348No Hit
GCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATG3860.10488018932776147No Hit
GCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACG3860.10488018932776147No Hit
GAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAAGGAATACTAAC3860.10488018932776147No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG3850.10460847899271544No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAGATACAGGCCTCT3850.10460847899271544No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3840.10433676865766943No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3810.10352163765253138No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3790.10297821698243936No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA3750.10189137564225531No Hit
ATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC3750.10189137564225531No Hit
TCACCATACTGCACTCAGGCAAGCAATACTATGCTGGGGGGAACTAATGA3750.10189137564225531No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG3730.10134795497216327No Hit
TGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTAT3730.10134795497216327No Hit
CGGGAACCTCAATGTTAGTATTCCTTGGACTCATAAAGTGGGTAACTTTA3710.10080453430207124No Hit
TCCTAGTACAAAGACCTTTAATCTAGTCTCCTCCCCCAACTCCTCCCAGT3710.10080453430207124No Hit
CTCCACAACTTTCCACCAAACTCTGCAAGATCCCAGGGTGAGAGGCCTGT3700.10053282396702524No Hit
CCTTAGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCA3700.10053282396702524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)