FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368039
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC25460.6917745130271521No Hit
CCTCCAGCTTATAGACCACTAAATGCCCCTATCTTATCAACACTTCCGGA23870.6485725697548358No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG23110.6279225842913387No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA15590.42359641233673606No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCATCAGTTGG13230.35947277326587673No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG12600.34235502215797786No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTGATTCCAGGATCTTCAACCACC11510.3127385956379623No Hit
ATATCAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT10560.28692611380859095No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA10410.2828504587829007No Hit
CTCTATGGAAGGCGGGTGTATTATATCAGAGAGAAACAACACGTAGCGCC10350.2812201967726246No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9770.2654609973399558No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9110.24752811522691887No Hit
CCTCAAGCTTATAGACCACTAAATGCCCCTATCTTATCAACACTTCCGGA9000.2445393015414127No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC8900.24182219819095258No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG8760.23801825350030836No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA8570.23285575713443413No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA8520.23149720545920408No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA8450.22959523311388194No Hit
CTCTTATAAAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7680.20867353731533886No Hit
GAGTATTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGG7380.20052222726395846No Hit
CTCTAATTCCAGGATCTTCAACCACCAGCGCGGGACCATGCAGAACCTGC7320.19889196525368236No Hit
ATCAGGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC7280.19780512391349828No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7190.19535973089808417No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7100.19291433788267004No Hit
ATACTATGCTGGGGGGAACTAATGACTCTAGCTACCTGGGTGGGTGTTAA7070.19209920687753199No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT6960.18911039319202586No Hit
GATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGG6930.1882952621868878No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6620.17987224180046135No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC6610.17960053146541535No Hit
GAATCAGAGGACAGACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC6530.17742684878504722No Hit
GGACAGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGTTTCCATGG6490.17634000744486317No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC6480.17606829710981717No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGTCCACT6480.17606829710981717No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6340.17226435241917298No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC6280.17063409040889688No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6270.17036238007385088No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6240.16954724906871282No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6170.16764527672339072No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTCGA6030.16384133203274653No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6010.16329791136265448No Hit
CTCTGATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5970.16221107002247043No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG5850.1589505460019183No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5770.15677686332155016No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG5730.1556900219813661No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5690.15460318064118206No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA5680.15433147030613603No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5680.15433147030613603No Hit
AGTCTAGACTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5600.15215778762576793No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5540.15052752561549185No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5360.14563673958466358No Hit
ATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGACT5340.14509331891457156No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT5150.13993082254869726No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5140.13965911221365127No Hit
GTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCG5090.1383005605384212No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5090.1383005605384212No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5050.13721371919823713No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5050.13721371919823713No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5040.13694200886319113No Hit
TATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCG5030.1366702985281451No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4940.13422490551273097No Hit
GTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATAC4940.13422490551273097No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC4880.13259464350245492No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC4850.13177951249731687No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4820.13096438149217882No Hit
ATACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4760.12933411948190274No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT4730.12851898847676468No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4630.12580188512630455No Hit
CATTTACTGTGAGTGGACCTACAAATTGTTCACATTTTTTGATAATGTCT4510.1225413611057524No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC4490.12199794043566035No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4470.12145451976556833No Hit
ATATCAGAGAGAAACAACACGAAGCGCCTCATTTTGTGGGTCACCATATT4450.12091109909547629No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC4450.12091109909547629No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4440.12063938876043029No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4380.11900912675015418No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT4260.11574860272960204No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT4250.11547689239455601No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG4240.11520518205951No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA4200.11411834071932594No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAAC4150.11275978904409588No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4140.11248807870904987No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4120.11194465803895783No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4120.11194465803895783No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT4110.11167294770391181No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG4080.11085781669877377No Hit
GAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGG4050.11004268569363572No Hit
GTATGCATGTATTCAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACA4030.10949926502354371No Hit
CTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA4010.10895584435345167No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3980.10814071334831364No Hit
GTGCTGGTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAG3940.10705387200812957No Hit
CCTTTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG3930.10678216167308355No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3900.10596703066794552No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA3870.10515189966280747No Hit
GAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAAGGAATACTAAC3860.10488018932776147No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA3850.10460847899271544No Hit
GTATTATATCAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACC3820.10379334798757742No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT3810.10352163765253138No Hit
AGGAATCCTGATGTGATGTTCTCCATGTTCAGCGCAGGGTCCCCAATCCT3800.10324992731748538No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAACAGGCTTTCACTTT3790.10297821698243936No Hit
TCTCGGGGCCGCTTGGGGATCTTTCGTCCCCTTCTCCGTCTGCCGTTTCG3790.10297821698243936No Hit
CTTATAAAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3780.10270650664739335No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3780.10270650664739335No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC3750.10189137564225531No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3690.10026111363197923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)