FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594302
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA28700.48291945845714807No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23660.39811409014272203No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23320.39239309307389175No Hit
CTCTATGACTGTGTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA20630.34712991038226354No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC19980.33619271010361734No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC18950.3188614542774549No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG16500.27763662245794224No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG14760.24835857863510472No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13230.22261409182536823No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12600.21201342078606497No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC11890.2000666327893899No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11830.19905704507136102No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11760.19787919273366064No Hit
GCACTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT10720.18037967228782673No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC10450.17583652755669676No Hit
GCGTTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT10220.1719664413042527No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10080.169610736628852No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9900.16658197347476536No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCT9530.16035618254692058No Hit
GTTCAACGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGGGAGAGG9490.15968312406823465No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCAAACAGAAA9440.15884180096987727No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT9330.156990890153491No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA9300.15648609629447655No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT9290.15631783167480506No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG9260.15581303781579062No Hit
GTTCAGTGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGGGAGAGG9140.15379386237973286No Hit
ATGTTGGATCGTCCAAATTACCACCCACCCAGGTAGCTAGAGTCATTAGT8970.1509333638453177No Hit
TCACCATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGGAACTAATGA8930.1502603053666318No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTGCACTCAGGCA8770.1475680714518881No Hit
ACTTTACCTGTCTTTAACCCTCGTTGGAAAACACCCTCTTTTCCTAATAT8680.14605368987484477No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8680.14605368987484477No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT8570.1442027790584585No Hit
ACATAGAGGTTCCTTGAGCAGTGGTCGTGCAGGTTCTGCATGGTCCCGCG8450.14218360362240073No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8380.1410057512847004No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTGAATTTCAT8250.13881831122897112No Hit
GGTACAGTAGAAGAATAAAGCCCCGTAAAGTTACCTACTTTATGAGTCCA8150.13713566503225633No Hit
CTAACATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGT8100.13629434193389892No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGG7930.13343384339948378No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC7820.13158293258309747No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAACGAGGGTTAAAGA7670.12905896328802527No Hit
TCCTTGAGCAGTGGTCGTGCAGGTTCTGCATGGTCCCGCGCTGGTGGTTG7590.12771284633065344No Hit
ATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATTC7530.12670325861262455No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT7490.12603020013393862No Hit
ATCCAACATCTAGGGATCTAGTAGTCAGTTATGTCAACACTAATATGGGC7430.12502061241590975No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC7390.12434755393722384No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7280.12249664312083755No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT7270.12232837850116607No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7210.12131879078313718No Hit
GGTAAAGTAGAAGAATAAAGCCCCGTAAAGTTACCTACTTTATGAGTCCA7200.12115052616346572No Hit
GTACAGATTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7200.12115052616346572No Hit
CAATAACATAGCCCATGAAATTCAAAGAGTAACCCCATCTTTTAGTTTTG7190.12098226154379423No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7050.11862655686839352No Hit
ATCCCAGGGTGAGGGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA6990.11761696915036464No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6950.11694391067167871No Hit
CCCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTCCACTGCATG6940.11677564605200721No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6910.11627085219299278No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6770.11391514751759206No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6770.11391514751759206No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6770.11391514751759206No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC6750.11357861827824911No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6710.11290555979956318No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC6690.11256903056022023No Hit
CTTCTAGATACCGCCTCAGCTCTGTTTCGGGATGCCTTAGAGTCTCCTGA6520.10970853202580506No Hit
ACTGTACCTGTCTTTAACCCTCGTTGGAAAACACCCTCTTTTCCTAATAT6510.1095402674061336No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6380.10735282735040434No Hit
ATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGGAACTAATGACTCTA6320.10634323963237546No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6300.10600671039303249No Hit
GTCTTTAACCCTCGTTGGAAAACACCCTCTTTTCCTAATATACATTTACA6250.10516538729467509No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT6230.10482885805533214No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6210.10449232881598917No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCCCT6180.10398753495697473No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT6110.10280968261927438No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT6090.1024731533799314No Hit
AATCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5980.10062224256354513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)