FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594302
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA28320.4765254029096318No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23680.398450619382065No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21350.35924496299861014No Hit
CTCTATGACTGTGTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA20100.33821188553967513No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC18730.31515963264468233No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC16770.28217976718907223No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG15830.2663628929399531No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG15510.26097842511046576No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12560.21134036230737907No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11930.2007396912680758No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11430.19232646028450182No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC11400.19182166642548737No Hit
GCGTTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT10160.17095685358622384No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9860.16590891499607943No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9730.1637214749403502No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG9540.1605244471665921No Hit
GCACTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT9510.16001965330757764No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT9310.15665436091414803No Hit
GTTCAACGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGGGAGAGG9020.1517746869436751No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT8980.15110162846498917No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCT8780.1477363360715596No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTGCACTCAGGCA8610.14487583753714442No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCAAACAGAAA8510.14319319134042963No Hit
ATGTTGGATCGTCCAAATTACCACCCACCCAGGTAGCTAGAGTCATTAGT8470.1425201328617437No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8470.1425201328617437No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC8350.14050095742568594No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8170.1374721942715993No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC8120.13663087117324188No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGG8050.13545301883554153No Hit
ACTTTACCTGTCTTTAACCCTCGTTGGAAAACACCCTCTTTTCCTAATAT7950.1337703726388267No Hit
GGTACAGTAGAAGAATAAAGCCCCGTAAAGTTACCTACTTTATGAGTCCA7920.1332655787798123No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT7870.13242425568145488No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTGAATTTCAT7830.13175119720276895No Hit
GTTCAGTGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGGGAGAGG7810.13141466796342602No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA7710.1297320217667112No Hit
TCACCATACTGCACTCAGGCAAGCAATACTTTGCTGGGGGGAACTAATGA7700.12956375714703972No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7400.12451581855689531No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC7370.12401102469788088No Hit
CTAACATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGT7310.123001436979852No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7310.123001436979852No Hit
ACATAGAGGTTCCTTGAGCAGTGGTCGTGCAGGTTCTGCATGGTCCCGCG7270.12232837850116607No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAACGAGGGTTAAAGA7110.1196361445864224No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT6990.11761696915036464No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC6950.11694391067167871No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC6880.11576605833397834No Hit
GGTAAAGTAGAAGAATAAAGCCCCGTAAAGTTACCTACTTTATGAGTCCA6680.11240076594054875No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6680.11240076594054875No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6600.1110546489831769No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6570.11054985512416247No Hit
CTTCTAGATACCGCCTCAGCTCTGTTTCGGGATGCCTTAGAGTCTCCTGA6550.1102133258848195No Hit
GTACAGATTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6530.10987679664547655No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT6460.10869894430777617No Hit
ATCCAACATCTAGGGATCTAGTAGTCAGTTATGTCAACACTAATATGGGC6440.10836241506843322No Hit
CAATAACATAGCCCATGAAATTCAAAGAGTAACCCCATCTTTTAGTTTTG6440.10836241506843322No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6430.10819415044876174No Hit
ATCCCAGGGTGAGGGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA6370.10718456273073286No Hit
CCCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTCCACTGCATG6360.10701629811106136No Hit
GTCTTTAACCCTCGTTGGAAAACACCCTCTTTTCCTAATATACATTTACA6240.1049971226750036No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6070.10213662414058845No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6070.10213662414058845No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6040.10163183028157402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)