FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510539
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC30910.6054385659077955No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG17360.3400327888760702No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17060.33415664621116115No Hit
GTATAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAACAAG14480.28362181929294333No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC13450.26344706281008895No Hit
ATATTAGAGTTAACATAACTGACTACTAGGTCCCTGGATGGTTGATCTTC13050.25561220592354356No Hit
TCCATAGAGTATGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTATC12740.24954019183647086No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT12650.24777734903699816No Hit
CTCTAATATGGGCCTAAAATTCAGGCAACTATTGTGGTTTCACCTTTCTT12630.2473856061926709No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC12610.24699386334834358No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12080.23661267797367097No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC11410.2234892926887074No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10880.2131081073140348No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10830.21212875020321662No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10630.2082113217599439No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC10100.19783013638527128No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT9930.19450032220848945No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA9790.19175812229819858No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT9690.1897994080765622No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC9490.18588197963328953No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9390.18392326541165316No Hit
GTCCTATACTTCCAAACACCTCAACCACCAGCACGGGACCATGCAGAACC9140.17902647985756232No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGCACG9050.1772636370580896No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGTTGAAGA9000.17628427994727142No Hit
GTTCTGGATAATAAGGTTTAATGCCCTTATCCAATGGCAAATATTTGGTA8770.17177923723750782No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8570.16786180879423512No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG8410.16472786603961695No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8390.16433612319528967No Hit
GAATATTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA8380.16414025177312605No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8240.16139805186283515No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8150.15963520906336243No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8130.15924346621903518No Hit
CCCTTATCCAATGGCAAATATTTGGTAGCATTTGGATAAAACCTAGCAGG7980.15630539488658066No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7880.1543466806649443No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7840.15356319497628976No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7810.15297558070979886No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7780.15238796644330796No Hit
CTCTATGACCGTTTCTCTTCCAAAGGTGAGACAAGAAAGGTGAAACCACA7600.1488622808443625No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7500.1469035666227262No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7440.14572833808974436No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC7390.1447489809789262No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCAGGCAACGGCCAGGTC7360.1441613667124353No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT7270.1423985239129626No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT7230.14161503822430804No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTACGCATGTATT6990.1369141240923808No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6930.135738895559399No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6870.13456366702641717No Hit
TCCTCAGGCCGCACAGTGGACTTCCACAACCTTCCACCAAACTCTGCAAG6820.133584309915599No Hit
GTACCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCACACTG6710.131429724271799No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6690.13103798142747175No Hit
GTCCCGTGCTGGTGGTTGAGGTGTTTGGAAGTATAGGACAAACGGGCAAC6600.12927513862799903No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6540.12809991009501723No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAAT6530.12790403867285358No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTA6520.12770816725068995No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC6520.12770816725068995No Hit
GGATAAGGGCATTAAACCTTATTATCCAGAACAGATAGTTAATCATTACT6370.12477009591823543No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCACACTGCACTCAGGCA6350.12437835307390817No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6340.12418248165174453No Hit
CTTCTAATGTTCCTGTCTTCAACCCTCATTGGAAAACACCATCTTTTCCT6330.12398661022958091No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC6060.11869808183116275No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT6020.11791459614250821No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5940.11634762476519912No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG5810.11380129627707188No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5810.11380129627707188No Hit
CCTTATGAGTCCAAGGAATATTAACATTGAGATCCCCGAGATTGAGATCT5770.11301781058841733No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5700.1116467106332719No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5690.11145083921110827No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACCGCATCTTGAGTCCCTTTTTACC5680.11125496778894461No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5670.11105909636678099No Hit
CCTTTACCCCGTTGCCAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5660.11086322494461735No Hit
ATCCAGAACAGATAGTTAATCATTACTTCCTAACCAGACATTATTTACAT5650.11066735352245373No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5650.11066735352245373No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5630.11027561067812645No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGT5630.11027561067812645No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGGA5590.1094921249894719No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTATACTTCCAAACACCTCAACCACC5520.10812102503432648No Hit
GAATACATGCGTACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5470.10714166792350828No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTACGCATGTATTCAATCTAAGC5440.1065540536570174No Hit
GTCCAAGGAATATTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGAC5420.10616231081269012No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5420.10616231081269012No Hit
CATTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGA5410.10596643939052648No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5380.10537882512403557No Hit
TCCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGT5320.10420359659105377No Hit
CCTCACCACACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAGCTAAT5320.10420359659105377No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5310.10400772516889015No Hit
GGTGGAGACAGAGGGGTAGGCCGCCTTCCGGACTGGCGATTGGTAGAGGC5290.10361598232456286No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTAATG5290.10361598232456286No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5280.10342011090239923No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT5280.10342011090239923No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5280.10342011090239923No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5250.10283249663590832No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA5240.10263662521374468No Hit
GCTCTGTACCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA5180.10146139668076289No Hit
CAGTAAACCCTGTTCCGACTACTGTATCTCACATATCGTCAATCTTCTCG5120.10028616814778107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)