FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510539
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG20240.39644375845919705No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16240.3180951895937431No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14950.2928277761346342No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC13070.2560039487678708No Hit
TCCATAGAGTATGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTATC12730.24934432041430724No Hit
CTCTAATATGGGCCTAAAATTCAGGCAACTATTGTGGTTTCACCTTTCTT12710.24895257756998No Hit
GTATAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAACAAG12310.24111772068343457No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC12100.23700442081799825No Hit
ATATTAGAGTTAACATAACTGACTACTAGGTCCCTGGATGGTTGATCTTC11240.22015947851192563No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10880.2131081073140348No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT10850.2125204930475439No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10820.21193287878105296No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT10680.20919067887076206No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGCACG10060.1970466506966167No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9620.18842830812141678No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9400.1841191368338168No Hit
GTCCTATACTTCCAAACACCTCAACCACCAGCACGGGACCATGCAGAACC9100.17824299416890776No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTCAT8870.17373795145914417No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8740.1711916229710169No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGTTGAAGA8600.16844942306072602No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA8470.16590309457259877No Hit
CCCTTATCCAATGGCAAATATTTGGTAGCATTTGGATAAAACCTAGCAGG8460.16570722315043515No Hit
GTTCTGGATAATAAGGTTTAATGCCCTTATCCAATGGCAAATATTTGGTA8260.16178979470716243No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8010.15689300915307156No Hit
GAATATTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA7980.15630539488658066No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7880.1543466806649443No Hit
CTCTATGACCGTTTCTCTTCCAAAGGTGAGACAAGAAAGGTGAAACCACA7800.15277970928763523No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7790.15258383786547158No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCAGGCAACGGCCAGGTC7730.15140860933248979No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7720.15121273791032613No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTACGCATGTATT7310.14318200960161712No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGGA7280.14259439533512622No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT7270.1423985239129626No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7210.14122329537998077No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7200.14102742395781714No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7200.14102742395781714No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7060.13828522404752625No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC7060.13828522404752625No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7040.13789348120319897No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC6930.135738895559399No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6920.13554302413723535No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6790.1329966956491081No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC6770.13260495280478082No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT6680.13084211000530813No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6670.1306462385831445No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6630.12986275289448995No Hit
CCTTTACCCCGTTGCCAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6600.12927513862799903No Hit
GGATAAGGGCATTAAACCTTATTATCCAGAACAGATAGTTAATCATTACT6570.12868752436150813No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6540.12809991009501723No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6400.12535771018472633No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCACACTGCACTCAGGCA6360.12457422449607179No Hit
TCCTCAGGCCGCACAGTGGACTTCCACAACCTTCCACCAAACTCTGCAAG6330.12398661022958091No Hit
GTACCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCACACTG6280.1230072531187627No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6190.12124441031929No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6110.11967743894198092No Hit
GTCCCGTGCTGGTGGTTGAGGTGTTTGGAAGTATAGGACAAACGGGCAAC6090.11928569609765366No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACCGCATCTTGAGTCCCTTTTTACC6060.11869808183116275No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC6050.11850221040899912No Hit
GCCTAATCACCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6010.11771872472034457No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTA5960.11673936760952641No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5860.11478065338789006No Hit
CCTCACCACACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAGCTAAT5830.11419303912139915No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT5750.11262606774409006No Hit
ATCCAGAACAGATAGTTAATCATTACTTCCTAACCAGACATTATTTACAT5740.11243019632192644No Hit
CCTTATGAGTCCAAGGAATATTAACATTGAGATCCCCGAGATTGAGATCT5730.11223432489976279No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5700.1116467106332719No Hit
CTTCTAATGTTCCTGTCTTCAACCCTCATTGGAAAACACCATCTTTTCCT5640.11047148210029008No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGT5640.11047148210029008No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5630.11027561067812645No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5610.10988386783379919No Hit
GCTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG5600.10968799641163554No Hit
CATTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGA5560.10890451072298099No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5550.10870863930081737No Hit
CAGTAAACCCTGTTCCGACTACTGTATCTCACATATCGTCAATCTTCTCG5540.10851276787865374No Hit
CCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5530.1083168964564901No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5530.1083168964564901No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5520.10812102503432648No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5450.10674992507918102No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5430.10635818223485374No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5410.10596643939052648No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5400.10577056796836286No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTACGCATGTATTCAATCTAAGC5380.10537882512403557No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG5370.10518295370187195No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5310.10400772516889015No Hit
GTCCAAGGAATATTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGAC5300.1038118537467265No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5180.10146139668076289No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5170.10126552525859923No Hit
TCTCGGGGCCGCTTGGGACTCACTCGTCCCCTTCTCCGTCTGCCGTTTCG5170.10126552525859923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)