FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559263
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTCGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT27850.4979768016121217No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC26930.481526580517574No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA25090.44862613832847875No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG20350.3638717383413528No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT20330.36351412483929746No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC19460.34795793749988824No Hit
TTCTACGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT18700.33436862442178367No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG18160.3247130598662883No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA13820.2471109299202701No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG13580.24281956789560546No Hit
TCCTCAGGCCATGCAGTGGAACTCAACCACTTTCCATCAAACTCTTCAAG13490.24121030713635622No Hit
ATGTAAGACCTTGGGCAAGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC12600.2252965062948917No Hit
GTGTAAAAGGGGCAGCGAACCCTAAAAGACCCACAATTCGTTGACATACT12220.21850184975583936No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT12150.21725020249864554No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC12080.2159985552414517No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAA11920.2131376472250086No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11710.2093827054534271No Hit
CATTTAAACCCTCACAAAACGAAAAGATGGGGATATTCCCTTAATTTCAT11650.20830986494726095No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCAACCACTTT11640.2081310581962333No Hit
ATATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTG11600.2074158311921225No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT11590.20723702444109482No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT11560.20670060418801173No Hit
GGATAATAAGGTTTGATACCTTTATCTAAGGGCAAGTATTTGGTAACATT11510.20580657043287326No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG11460.20491253667773482No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCAACACGTAGCGCC11320.2024092421633471No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA11270.20151520840820863No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTA11140.19919072064484866No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCAGGAAGTAATGATTAACTACA11110.1986543003917656No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATCAATTTTAATC11050.19758145988559944No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10880.1945417451181287No Hit
ATATAAAAGAGAGTCAACACGTAGCGCCTCATTCTTCGGGTCACCATATT10390.18578021431777178No Hit
CCATTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA10390.18578021431777178No Hit
GTATGGATGGGCAGAGGAGACACAAAGGTGCCACGCATGCGCTGATGGCC10200.18238288604824562No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9930.17755510377049796No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG9640.1723697079906949No Hit
CCTCCATATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGAAAC9430.16861476621911337No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA9400.1680783459660303No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9400.1680783459660303No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG9390.16789953921500259No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA9380.1677207324639749No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT9190.16432340419444877No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9140.1634293704393103No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA9000.1609260759249226No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCTGACGAGACATTATT8970.16038965567183955No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC8970.16038965567183955No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTCCATATAGACCAGCAAATGCC8930.15967442866772877No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT8920.1594956219167011No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG8680.15520425989203648No Hit
CCTTTTTGGCATCCGGCCAGTTGTCCTTGTATGGATTGAGGTCCCAATCT8680.15520425989203648No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGTTTT8420.1505552843653165No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA8390.15001886411223342No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAATGGAGTAGGCTGCCT8370.14966125061017804No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA8360.14948244385915035No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT8310.1485884101040119No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG8280.1480519898509288No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC8170.1460851155896242No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8070.1442970480793473No Hit
GTATAAATGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC8040.1437606278262642No Hit
TATTAACATTGACATAGCCGACTACTAATTCCCTGGATGCTGGGTCTTCC8000.14304540082215345No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG7990.14286659407112576No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA7970.14250898056907035No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG7950.14215136706701498No Hit
GGTCTATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATA7940.1419725603159873No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7940.1419725603159873No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTTAGATCT7910.14143614006290423No Hit
TCCTAATACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCACT7910.14143614006290423No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG7830.14000568605468267No Hit
ACCAAATACTTGCCCTTAGATAAAGGTATCAAACCTTATTATCCAGAGCA7770.1389328455485165No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTT7720.13803881179337807No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA7720.13803881179337807No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA7550.13499909702590732No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATTCCTATGGGAG7550.13499909702590732No Hit
TTCTACGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCT7510.13428387002179654No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC7480.13374744976871347No Hit
CCCTAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATGGGCCTGT7420.13267460926254732No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC7380.13195938225843654No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7360.13160176875638116No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTGTGCCTAAC7310.1307077350012427No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAGGTAATTTGG7290.13035012149918732No Hit
CCTTGTTGGCATCCGGCCAGTTGTCCTTGTGTGGATTGAGGTCCCAATCT7280.13017131474815963No Hit
ATGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCC7170.128204440486855No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7090.1267739864786335No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCTAAAAGACCCACAATTCG7090.1267739864786335No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7070.1264163729765781No Hit
GGATTAAAGTTAGGCACAGAAGAAGAATAAAGCCCCGTAAAGTTTCCCAC7070.1264163729765781No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTGACGT7010.12534353247041194No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG7000.12516472571938428No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG7000.12516472571938428No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTTAGATCTTCTGCGAC7000.12516472571938428No Hit
GGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGCATAAAAATCAGA6990.12498591896835656No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTGATTATGCCTGCTAGA6980.12480711221732887No Hit
CAGTTGGACCCTGCATTCAAAGCCAACACAGACAATCCAGATTGGGACCT6940.12409188521321811No Hit
CCCGAAGAATGAGGCGCTACGTGTTGACTCTCTTTTATATAAGATACCCG6920.12373427171116272No Hit
CCAACAAGGTGGGAGTGGGAGCATTCGGGCCAGGGTTCACCCCTCCCCAT6910.12355546496013504No Hit
TTCTTCTTCTGTGCCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC6840.12230381770294119No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT6820.12194620420088581No Hit
GACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTAGCT6750.12069455694369195No Hit
GCTTTATTCTTCTTCTGTGCCTAACTTTAATCCTGATTGGCAAACTCCTT6640.11872768268238736No Hit
GGGTTCACCCCTCCCCATGGGGGCTTGTTGGGGTGGAGCCCTCAGGCTCA6630.11854887593135965No Hit
TGTATGGATTGAGGTCCCAATCTGGATTGTCTGTGTTGGCTTTGAATGCA6600.11801245567827658No Hit
CCTGTAAACAGGCCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6580.11765484217622121No Hit
AGTCTGTACAACATCTTGAGTCCATTTATACCGCTGTTACCAATTTTCTT6560.11729722867416582No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6490.11604558141697198No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6460.1155091611638889No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA6460.1155091611638889No Hit
GAATACTAACATTGAGATTCCCGAGATTTAGATCTTCTGCGACGCGGTGA6420.11479393415977812No Hit
CCCGCAGAATGAGGCGCTACGTGTTGACTCTCTTTTATATAAGATACCCG6400.11443632065772276No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG6340.1133634801515566No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6320.11300586664950121No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC6290.11246944639641814No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTCGTTT6270.11211183289436277No Hit
CCTTAGAGGTGGAGATAATGGAGTAGGCTGCCTTCCTGACTGCCGATTGG6260.11193302614333506No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6260.11193302614333506No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA6240.1115754126412797No Hit
ATGTAATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA6180.11050257213511354No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG6130.10960853837997508No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC6060.10835689112278124No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCATTTATACC5970.10674763036353202No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAGGTAA5950.10639001686147663No Hit
GAGGATGAGTGTCCCTTAGAGGTGGAGATAATGGAGTAGGCTGCCTTCCT5810.10388672234708893No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGG5790.10352910884503357No Hit
GGGCTGTACCGCCCGCTTCTCCGCCTGCCGTACCGACCGACCACGGGGCG5760.10299268859195049No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT5740.1026350750898951No Hit
CTCATCCTCAGGCCATGCAGTGGAACTCAACCACTTTCCATCAAACTCTT5710.10209865483681202No Hit
CCTCCTCCATATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA5710.10209865483681202No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA5700.10191984808578433No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5690.10174104133475663No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT5640.10084700757961819No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG5630.10066820082859047No Hit
CCTTTAACCTCATCTCCTCCCCCAACTCCTCCCACTCAGCAAACACACAG5600.10013178057550741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)