FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477542
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA21230.4445682264596622No Hit
TGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA20830.4361919998659804No Hit
TCTTTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG19950.41776430135988035No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCCAGGGGGAACACCCGTG18940.39661432921083384No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC17580.3681351587923157No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG15570.32604462015906455No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT15550.32562580882938047No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT15540.3254164031645384No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14660.3069887046584384No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14660.3069887046584384No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA12280.2571501564260316No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG12280.2571501564260316No Hit
GTTGTGAGTAGGCCCTGCGCCTGAGGGCTCCACCCCAACAGTCCCCCATG11810.24730809017845548No Hit
GTACCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG11620.24332938254645664No Hit
GTATCGGGAGGGCTTAGAGTCTCCAGAACATTGTACACCTCACCATACGG11390.2385130522550896No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG11220.23495315595277486No Hit
GGATAATACGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTAACATT10720.22448287271067258No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG10380.21736308010604305No Hit
GTATAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG10320.21610664611699076No Hit
CCTCTTGCATATAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA10190.21338437247404415No Hit
TTATCTACACACTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCA10100.21149972149046575No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9900.20731160819362485No Hit
TCTCCATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGC9850.2062645798694146No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC8630.18071708875868508No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT8410.1761101641321601No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8060.1687809658626885No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7920.1658492865548999No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7850.16438344690100556No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACGTGGCCCCCAAAACCACATC7650.16019533360416466No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACATACGGGCAAC7620.15956711660963852No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC7600.15914830527995444No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT7420.15537900331279764No Hit
GGCTTAGAGTCTCCAGAACATTGTACACCTCACCATACGGCACTCAGGCA7400.15496019198311353No Hit
GATTTAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT7370.1543319749885874No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7290.15265672966985103No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA7270.15223791834016695No Hit
GGGCTCTACCGCCCGCTTCTCCGCCTATTGTACCGACCGACCACGGGGCG7240.1516097013456408No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGAAAAGAAGGAGTTT7130.1493062390323783No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7090.14846861637301012No Hit
GTTCATGTACTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6960.14574634273006354No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6940.14532753140037946No Hit
GTACAGACGTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA6910.14469931440585332No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6820.1428146634222749No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGA6700.14030179544417035No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6680.13988298411448627No Hit
ATATTAACGTTGACGTAGCCTACAACTAATTCCCTGGATGCTGGATCTTC6540.13695130480669762No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6420.1344384368285931No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6380.13360081416922492No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG6310.1321349745153306No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6270.13129735185596242No Hit
GTAGAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG6190.12962210653722606No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTTGGCTGCCT6110.1279468612184897No Hit
GTTTCGCCCCAGACCGGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA6110.1279468612184897No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6100.12773745555364763No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACACATAAGGTG6100.12773745555364763No Hit
GTACAGGGTCCCCAGTCTTCGATGAGATTGACGATATGGCAGAGACAGTA6080.12731864422396355No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA6050.1266904272294374No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5930.12417755925133286No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGA5910.12375874792164877No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5880.12313053092712264No Hit
CGTCCTTTGTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCC5880.12313053092712264No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGGAT5830.12208350260291241No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG5760.12061766294901809No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5650.11831420063575561No Hit
GTTGTAGGCTACGTCAACGTTAATATGGGCCTAAAATTCAGGCAACTATT5630.11789538930607152No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGAAAAGAAGGAGT5570.11663895531701923No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5530.11580133265765105No Hit
GAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGACCCTG5460.11433549300375674No Hit
GTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAA5460.11433549300375674No Hit
GCTCTGGATAATACGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTA5430.1137072760092306No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5430.1137072760092306No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5330.11161321936081015No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5290.11077559670144196No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5260.11014737970691582No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5230.1095191627123897No Hit
CCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGAAGATGATAAAA5230.1095191627123897No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5200.10889094571786355No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCCAGGGGG5150.10784391739365334No Hit
CAGAATACTGTCTCTGCCATATCGTCAATCTCATCGAAGACTGGGGACCC5140.10763451172881129No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5140.10763451172881129No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATCCCTATGGGAG5050.10574986074523288No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5040.10554045508039084No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4960.10386520976165448No Hit
CAGTACTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGA4940.10344639843197038No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCCAGGG4900.1026087757726022No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACA4900.1026087757726022No Hit
TTGTACACCTCACCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTG4880.10218996444291811No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4870.10198055877807606No Hit
ACATTGAGATTCCCGAGATTTAGATCTTCTGCGACGCGGCGATTGAGACC4840.10135234178354993No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4840.10135234178354993No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4830.10114293611870788No Hit
CCCATAGGGATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4790.1003053134593397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)