FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265965
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGGAGCACCCACG13130.4936739796589777No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12520.470738631022879No Hit
GTTGTTAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG11540.4338916774763597No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10160.3820051510537101No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10140.3812531724099036No Hit
ATATAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC9290.3492940800481266No Hit
GTATGTAAATAATGCCTAGCTTTAAAGTAATGATTAACTGCATGTTCAGG8510.3199669129396725No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT8270.31094316921399434No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7970.2996634895568966No Hit
GTGTAAAAGGTGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7940.2985355215911868No Hit
GTATACAGGCATATAACGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7890.29665557498167056No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT7330.2756001729550881No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7320.27522418363318485No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6970.2620645573665708No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6940.260936589400861No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6770.25454477092850564No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCGTTATATGCCTGTAT6640.24965690974376328No Hit
GTATCGGGAGGCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAG6330.23800124076476226No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6300.23687327279905251No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6220.23386535822382645No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA6050.22747353975147108No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCACTGCTAG5930.22296166788863198No Hit
CCTGTATACAATCAAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5910.22220968924482545No Hit
CATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG5870.2207057319572124No Hit
TTATATGCCTGTATACAATCAAAACAGGCTTTCACTTTCTCGCCAACTTA5460.20529016975917883No Hit
GGCATATAACGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAG5380.20228225518395276No Hit
GCCTGAGGATGACTGTCTCTTAGTGGTGGAGAGATGGGAGTAGGCTGTCT5040.18949861823924202No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4900.1842347677325964No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4870.18310679976688662No Hit
CTCTTATATAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTC4840.18197883180117685No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4630.1740830560412084No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4620.17370706671930516No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4600.17295508807549867No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGCCTTTGCCAAGTGTTTGCTG4570.17182712010978887No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4550.17107514146598235No Hit
CTTTAAAGCTAGGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTAT4540.1706991521440791No Hit
GATATAGGCCCCTCACTCTGGGATCTAGCAGTGCTTGGTGGAATGTTGTG4450.1673152482469498No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4430.16656326960314327No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4420.16618728028124002No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4420.16618728028124002No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4390.16505931231553025No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAGG4250.15979546180888463No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4220.15866749384317486No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4100.15415562198033575No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCCCCGGAACATTGTTCACCTCACCA4090.1537796326584325No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4080.15340364333652923No Hit
CTCTGGGATCTAGCAGTGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4070.15302765401462598No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGC4030.15152369672701296No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4010.15077171808320644No Hit
ATGTTAATATGGGCATAAAGATCAGACAAATATTGTGGTTTCACATTTCC3980.14964375011749664No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAGCTAGGCATTATT3960.14889177147369015No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3930.14776380350798038No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3930.14776380350798038No Hit
GTCCAAGGGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGAC3910.14701182486417386No Hit
GTTCTATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC3880.1458838568984641No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAACATAAGA3850.14475588893275432No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3810.14325193164514127No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT3790.14249995300133475No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3730.1402440170699152No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3720.13986802774801196No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC3710.1394920384261087No Hit
TCTGTGGAGTTGCTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG3710.1394920384261087No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTATTGGGGTTCAACTGTATA3700.13911604910420544No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGG3660.13761209181659242No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3640.1368601131727859No Hit
GCTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC3620.13610813452897938No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGG3620.13610813452897938No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTGCTCTCTTTTTTGCCTTCTG3610.1357321452070761No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTAGGCAAGACCTGGCGGGCGTTC3590.1349801665632696No Hit
ATCCCAGAGTGAGGGGCCTATATCTTCCTGCTGGTGGCTCCAGTTCCGGA3570.1342281879194631No Hit
GCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAGCAATCAGGCA3560.13385219859755984No Hit
GAATACAGGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3530.13272423063185004No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3470.1304682947004305No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATCCCCGAGATTGAGATCT3450.12971631605662398No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCC3420.1285883480909142No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGC3420.1285883480909142No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT3410.12821235876901094No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3410.12821235876901094No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3390.12746038012520444No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3350.12595642283759143No Hit
ATGTGGCTATCCTGCCTTGATGCCGTTATATGCCTGTATACAATCAAAAC3350.12595642283759143No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3300.12407647622807512No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3300.12407647622807512No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGC3300.12407647622807512No Hit
GTATACAATCAAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT3280.12332449758426861No Hit
CCCCAAAAGGCCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG3280.12332449758426861No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3280.12332449758426861No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA3270.12294850826236536No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3270.12294850826236536No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA3240.12182054029665557No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT3240.12182054029665557No Hit
TCCACAGAGTATGTAAATAATGCCTAGCTTTAAAGTAATGATTAACTGCA3230.12144455097475232No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG3210.12069257233094581No Hit
GGTTGGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3190.11994059368713929No Hit
ACATTCCACCAAGCACTGCTAGATCCCAGAGTGAGGGGCCTATATCTTCC3120.11730866843381647No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC3110.11693267911191323No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC3090.11618070046810672No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3070.1154287218243002No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3070.1154287218243002No Hit
GTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCCTCAT3060.11505273250239692No Hit
ATCTAGCAGTGCTTGGTGGAATGTTGTGGAGTTCCACTGCATGGCCTGAG3050.11467674318049367No Hit
GACAAATATTGTGGTTTCACATTTCCTGTCTTATGTTTGGAAGAGAAACT3040.11430075385859041No Hit
GTCTTATGTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCTTTTGGA3040.11430075385859041No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC3000.11279679657097738No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG2960.11129283928336435No Hit
GGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA2960.11129283928336435No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT2960.11129283928336435No Hit
CCATACAGCAATCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC2930.11016487131765458No Hit
GCCTATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTT2910.10941289267384806No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT2880.10828492470813827No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA2880.10828492470813827No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATAT2870.10790893538623503No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT2870.10790893538623503No Hit
GCCATGGGAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC2870.10790893538623503No Hit
GTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGTGAGGCAGTA2860.10753294606433177No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAGATATAGGCCCCT2840.10678096742052524No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2840.10678096742052524No Hit
ACTGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC2840.10678096742052524No Hit
CTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACATTCCA2820.10602898877671874No Hit
GTCTCTTAGTGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGAT2820.10602898877671874No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC2800.10527701013291224No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA2780.10452503148910572No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG2780.10452503148910572No Hit
GTGGTGGGGTGAACCCTGGCCCGAATGCTCCCGCTCCTACCTGATTTGCC2770.10414904216720244No Hit
GCCTACTCCCATCTCTCCACCACTAAGAGACAGTCATCCTCAGGCCATGC2770.10414904216720244No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC2760.1037730528452992No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGTGCTTGGTGGAATGTTGTGG2750.10339706352339594No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG2750.10339706352339594No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA2720.10226909555768617No Hit
GTGGAATGTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTA2710.1018931062357829No Hit
GCCTATGGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC2710.1018931062357829No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG2700.10151711691387966No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2690.10114112759197638No Hit
GTATACAGTTGAACCCCAATAAAACTAAACGTTGGGGCTACTCCCTTAAC2680.10076513827007313No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT2670.10038914894816987No Hit
GTTATATGCCTGTATACAATCAAAACAGGCTTTCACTTTCTCGCCAACTT2660.10001315962626663No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG2660.10001315962626663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)