FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265965
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGGAGCACCCACG12560.472242588310492No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10470.39366082003271113No Hit
GTTGTTAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG10450.39290884138890453No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10230.38463707630703287No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9580.3601977703833211No Hit
GTATGTAAATAATGCCTAGCTTTAAAGTAATGATTAACTGCATGTTCAGG8460.3180869663301562No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8330.3131991051454139No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT8320.3128231158235106No Hit
ATATAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7980.30003947887879984No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT7890.29665557498167056No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7310.2748481943112816No Hit
GTATACAGGCATATAACGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7130.26808038651702293No Hit
GTGTAAAAGGTGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7060.2654484612637001No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6360.23912920873047205No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCGTTATATGCCTGTAT6300.23687327279905251No Hit
GTATCGGGAGGCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAG6250.2349933261895362No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA6130.23048145432669714No Hit
CATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG6120.23010546500479384No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5890.22145771060101896No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5870.2207057319572124No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5830.21920177466959936No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCACTGCTAG5770.21694583873817985No Hit
TTATATGCCTGTATACAATCAAAACAGGCTTTCACTTTCTCGCCAACTTA5610.21093000958772773No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5360.20153027654014627No Hit
CCTGTATACAATCAAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5310.19965032993062995No Hit
GGCATATAACGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAG5100.19175455417066153No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5010.18837065027353223No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGCCTTTGCCAAGTGTTTGCTG4900.1842347677325964No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4890.18385877841069315No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4690.17633899197262798No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGC4670.17558701332882146No Hit
GCCTGAGGATGACTGTCTCTTAGTGGTGGAGAGATGGGAGTAGGCTGTCT4610.17333107739740192No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4610.17333107739740192No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4600.17295508807549867No Hit
CTTTAAAGCTAGGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTAT4590.1725790987535954No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4500.16919519485646609No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4400.1654353016374335No Hit
GATATAGGCCCCTCACTCTGGGATCTAGCAGTGCTTGGTGGAATGTTGTG4320.16242738706220744No Hit
CTCTTATATAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTC4240.15941947248698135No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4190.1575395258774651No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4180.1571635365555618No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4130.15528358994604555No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4000.15039572876130317No Hit
CTCTGGGATCTAGCAGTGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4000.15039572876130317No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3970.1492677607955934No Hit
GCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAGCAATCAGGCA3900.1466358355422706No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAGG3900.1466358355422706No Hit
GCTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC3880.1458838568984641No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3860.14513187825465756No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGG3810.14325193164514127No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3810.14325193164514127No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGG3800.14287594232323803No Hit
ATGTTAATATGGGCATAAAGATCAGACAAATATTGTGGTTTCACATTTCC3760.141371985035625No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3760.141371985035625No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3720.13986802774801196No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3700.13911604910420544No Hit
GTTCTATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC3690.13874005978230217No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCCCCGGAACATTGTTCACCTCACCA3640.1368601131727859No Hit
GTCCAAGGGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGAC3600.13535615588517286No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTATTGGGGTTCAACTGTATA3590.1349801665632696No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATCCCCGAGATTGAGATCT3560.13385219859755984No Hit
CCCCAAAAGGCCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG3530.13272423063185004No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3510.13197225198804352No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAACATAAGA3500.13159626266614027No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAGCTAGGCATTATT3480.13084428402233378No Hit
TCTGTGGAGTTGCTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG3440.12934032673472073No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3420.1285883480909142No Hit
ATCCCAGAGTGAGGGGCCTATATCTTCCTGCTGGTGGCTCCAGTTCCGGA3410.12821235876901094No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3380.1270843908033012No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTGCTCTCTTTTTTGCCTTCTG3350.12595642283759143No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTAGGCAAGACCTGGCGGGCGTTC3330.1252044441937849No Hit
GTCTCTTAGTGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGAT3320.12482845487188164No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3310.1244524655499784No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGC3280.12332449758426861No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3270.12294850826236536No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCC3260.12257251894046209No Hit
GAATACAGGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3260.12257251894046209No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3260.12257251894046209No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3250.12219652961855884No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG3210.12069257233094581No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT3210.12069257233094581No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC3190.11994059368713929No Hit
GTATACAATCAAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTT3160.1188126257214295No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3160.1188126257214295No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA3160.1188126257214295No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3150.11843663639952626No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3150.11843663639952626No Hit
TCCACAGAGTATGTAAATAATGCCTAGCTTTAAAGTAATGATTAACTGCA3090.11618070046810672No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3070.1154287218243002No Hit
GGTTGGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3070.1154287218243002No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA3040.11430075385859041No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC3040.11430075385859041No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC3010.11317278589288064No Hit
ATAGAGGCCCAGACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG2990.11242080724907413No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT2960.11129283928336435No Hit
GTCTTATGTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCTTTTGGA2920.1097888819957513No Hit
GTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCCTCAT2890.10866091403004155No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC2890.10866091403004155No Hit
GGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA2890.10866091403004155No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT2870.10790893538623503No Hit
CTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACATTCCA2870.10790893538623503No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2860.10753294606433177No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT2850.10715695674242852No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA2840.10678096742052524No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGC2810.10565299945481549No Hit
CCATACAGCAATCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC2800.10527701013291224No Hit
ACTGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC2800.10527701013291224No Hit
ATATTGTACTTAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATCGT2770.10414904216720244No Hit
GCCTATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTT2770.10414904216720244No Hit
GTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAGTGGTGG2740.10302107420149269No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG2740.10302107420149269No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2740.10302107420149269No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG2720.10226909555768617No Hit
ATCTAGCAGTGCTTGGTGGAATGTTGTGGAGTTCCACTGCATGGCCTGAG2720.10226909555768617No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGTGCTTGGTGGAATGTTGTGG2710.1018931062357829No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC2710.1018931062357829No Hit
GCCTACTCCCATCTCTCCACCACTAAGAGACAGTCATCCTCAGGCCATGC2710.1018931062357829No Hit
GTTATATGCCTGTATACAATCAAAACAGGCTTTCACTTTCTCGCCAACTT2690.10114112759197638No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG2660.10001315962626663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)