FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409553
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC22840.5576811792368753No Hit
GTTGTTAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG17050.4163075352884731No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15250.3723571796568454No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14540.35502120604659226No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13500.32962766723720743No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA12890.3147333800509336No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG12820.31302419955414806No Hit
CTGTCAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT12080.2949557200167011No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11930.29129319038073215No Hit
CCTCCCGCTTACAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGA11180.2729805422008873No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA11150.27224803627369354No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10840.26467880835935764No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10270.2507611957426755No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT10030.2449011483251252No Hit
CATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG9980.24368030511313551No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9930.24245946190114587No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9260.22610016286048448No Hit
GGGATTTAGTAGTCAGCTATGTCAATGTTAATATGGGCATAAAAATCAGA9200.22463515100609688No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9030.22048428408533205No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8760.21389173074058793No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT8500.20754334603824168No Hit
CTCTGACAGTCAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8460.20656667146864996No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8350.20388081640227274No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7410.18092896401686717No Hit
CTACTGTTGTTAGATCCCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG7280.17775477166569406No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTC7250.17702226573850027No Hit
ATCCTAACGTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7080.17287139881773544No Hit
TCCATCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGATTG7040.1718947242481437No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7000.17091804967855198No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6800.16603467683059334No Hit
CTCTTACAGTCAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6660.1626163158370223No Hit
GGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA6500.1587096175586554No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6460.15773294298906368No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6320.15431458199549267No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6220.15187289557151334No Hit
ATATTTGCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6200.15138455828671746No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6200.15138455828671746No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG6180.1508962210019216No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCATCAAATGCC6140.14991954643232988No Hit
GTTACCTGGATGATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAG6120.14943120914753402No Hit
CTCCCACCTGATTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC6020.14698952272355473No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5970.14576867951156505No Hit
CTCCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTAT5880.14357116172998366No Hit
GCGTTCTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT5860.1430828244451878No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTGACAGTCAATGAAAAA5820.14210614987559608No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5780.14112947530600436No Hit
GTAGAGGCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5760.14064113802120848No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5690.13893195752442297No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5600.13673443974284158No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5520.13478109060365814No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5500.13429275331886226No Hit
CCTTATGAGTCCACGGGATACTAACATTGAGATCCCCGAGATTGAGATCT5480.13380441603406643No Hit
GTCCACGGGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGAC5380.1313627296100871No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG5360.13087439232529122No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5350.13063022368289331No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5300.12940938047090364No Hit
ATCGCTGGATGTGTCTGCGGCGTTCTATCATATTCCTCTTCATCCTGCTG5280.12892104318610778No Hit
GTTCTGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGC5270.12867687454370985No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5250.128188537258914No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5190.1267235254045264No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5150.12574685083493467No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5100.12452600762294502No Hit
GATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATTGGAGGAC5050.12330516441095536No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5010.12232848984136363No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTC4950.12086347798697604No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4940.12061930934457812No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4910.11988680341738434No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4860.11866596020539467No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4860.11866596020539467No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4850.11842179156299673No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4800.11720094835100707No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4790.11695677970860914No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGC4770.11646844242381327No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4740.11573593649661948No Hit
GGCAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT4710.11500343056942569No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4690.11451509328462982No Hit
CGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4640.11329425007264018No Hit
GTTCTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG4630.11305008143024224No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4630.11305008143024224No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTGTTCG4620.11280591278784431No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4570.11158506957585465No Hit
GTTAGTATCCCGTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4550.11109673229105878No Hit
TCCATGTAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGA4550.11109673229105878No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC4550.11109673229105878No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4550.11109673229105878No Hit
GGTCTTGCATGGCCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4530.11060839500626292No Hit
TCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGA4520.11036422636386499No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4500.10987588907906912No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4490.1096317204366712No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4440.10841087722468154No Hit
ATGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCC4390.10719003401269188No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4390.10719003401269188No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4350.10621335944310016No Hit
GAATATGATAGAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATTG4340.10596919080070222No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA4300.1049925162311105No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4230.10328333573432498No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4190.10230666116473326No Hit
CTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTT4170.10181832387993739No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4170.10181832387993739No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4150.10132998659514153No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4140.1010858179527436No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4130.10084164931034567No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4120.10059748066794773No Hit
GCACGGGGCCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4110.10035331202554981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)