FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432865
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA24340.5623000242569854No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21420.494842502858859No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17080.3945802963972601No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA15820.3654719138761508No Hit
GCCACACAGTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGT13740.31741998082543055No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG13690.31626488628094207No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA12290.28392223903526503No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA11460.2647476695967565No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC10860.2508865350628949No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10400.24025966525360098No Hit
GTTGTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA9920.22917075762651173No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9200.21253739618587783No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTGCTTTACCACAG9010.20814803691682165No Hit
GCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGT8790.2030656209210724No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8370.19336282674736927No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT7590.1753433518533492No Hit
TTTCTAGGGGGAGCACCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAAC7490.17303316276437228No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7380.17049195476649764No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7120.1644854631351576No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT7120.1644854631351576No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG7030.16240629295507836No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6980.1612511984105899No Hit
ATATGTTCCTGTGGTAAAGCACCCCAACTTCCAATTACATATCCCATGAA6950.16055814168389684No Hit
GGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTG6950.16055814168389684No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG6940.16032712277499914No Hit
CTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6700.15478266896145448No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6620.15293451769027294No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6550.1513173853279891No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6480.14970025296570524No Hit
AGTTAAGGGAGTAGCCCCATCTTTTGGTTTTATTAGGGTTCAAATGTATA6400.14785210169452367No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6400.14785210169452367No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6310.14577293151444445No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6310.14577293151444445No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6120.14138357224538828No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6090.14069051551869521No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5980.13814930752082058No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5970.13791828861192287No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5900.13630115624963904No Hit
CATTTGAACCCTAATAAAACCAAAAGATGGGGCTACTCCCTTAACTTCAT5880.13583911843184365No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGCAG5750.13283587261617363No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5730.13237383479837825No Hit
GTCTGCACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT5620.12983262680050361No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5620.12983262680050361No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5600.12937058898270823No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTC5530.12775345662042437No Hit
GTCCAAGAGTTCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5500.1270603998937313No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGCACCCCAACTTCCAATTACA5460.12613632425814053No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5460.12613632425814053No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5440.12567428644034515No Hit
CCCTCGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTG5430.12544326753144744No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5410.12498122971365205No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA5330.12313307844247053No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCCGCCGGACCGTGTG5270.12174696498908436No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5170.11943677590010743No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5110.11805066244672126No Hit
GTTAAGGGAGTAGCCCCATCTTTTGGTTTTATTAGGGTTCAAATGTATAC5110.11805066244672126No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5030.11620251117553972No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5020.11597149226664204No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5010.11574047335774433No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4990.11527843553994893No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4970.11481639772215357No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC4950.11435435990435817No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4850.11204417081538123No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4790.11065805736199508No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4780.11042703845309737No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4770.11019601954419969No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCGGATGAGAAGGCACAG4770.11019601954419969No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4750.1097339817264043No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4750.1097339817264043No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4740.10950296281750661No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4740.10950296281750661No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4730.1092719439086089No Hit
GTCTTACATAAGAGAACTCTTGGACTCTCAGCGATGTCAACGACCGACCT4720.10904092499971123No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4720.10904092499971123No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4710.10880990609081352No Hit
GATTTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCCCTGG4660.10765481154632506No Hit
GGAGCACAACTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATA4650.10742379263742738No Hit
CACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAG4630.10696175481963198No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4620.1067307359107343No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT4580.10580666027514352No Hit
CAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGG4570.10557564136624584No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4570.10557564136624584No Hit
CATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACACGCAG4520.10442054682175736No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC4480.10349647118616659No Hit
CCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAAC4470.10326545227726888No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4450.1028034144594735No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC4410.10187933882388274No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4410.10187933882388274No Hit
GTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4360.10072424427939428No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4350.10049322537049657No Hit
CACAAATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAG4330.10003118755270118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)