FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432865
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA22210.5130929966617768No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21390.49414944613216594No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA14030.3241195291834637No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13640.3151097917364536No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG12900.29801439247802436No Hit
GCCACACAGTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGT11220.25920321578321187No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11210.25897219687431416No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA11090.25619996996754185No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTGCTTTACCACAG9230.21323045291257087No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC8750.20214154528548162No Hit
GTTGTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA8590.19844524274311853No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8390.19382486456516465No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA8120.18758735402492696No Hit
GCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGT7850.18134984348468922No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7720.17834659766901922No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7550.1744192762177584No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7490.17303316276437228No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7380.17049195476649764No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT7070.16333036859066916No Hit
TTTCTAGGGGGAGCACCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAAC7050.16286833077287377No Hit
CTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG7000.1617132362283853No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6940.16032712277499914No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6650.15362757441696603No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6640.15339655550806836No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG6620.15293451769027294No Hit
ATATGTTCCTGTGGTAAAGCACCCCAACTTCCAATTACATATCCCATGAA6500.15016229078350063No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6410.14808312060342138No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT6340.14646598824113755No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT6180.14276968569877443No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCCGCCGGACCGTGTG6140.14184561006318366No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6130.14161459115428598No Hit
AGTTAAGGGAGTAGCCCCATCTTTTGGTTTTATTAGGGTTCAAATGTATA6120.14138357224538828No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5990.1383803264297183No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG5830.13468402388735518No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5800.1339909671606621No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5780.13352892934286673No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5780.13352892934286673No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5750.13283587261617363No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5740.13260485370727593No Hit
CATTTGAACCCTAATAAAACCAAAAGATGGGGCTACTCCCTTAACTTCAT5690.13144975916278748No Hit
GTCTTACATAAGAGAACTCTTGGACTCTCAGCGATGTCAACGACCGACCT5680.1312187402538898No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5580.12890855116491284No Hit
GGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTG5490.12682938098483362No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGCACCCCAACTTCCAATTACA5430.12544326753144744No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5310.12267104062467513No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5290.12220900280687974No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5260.12151594608018666No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5240.12105390826239128No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGCAG5220.12059187044459589No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5190.11989881371790281No Hit
GTCTGCACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT5110.11805066244672126No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5050.1166645489933351No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5020.11597149226664204No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5000.11550945444884664No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4990.11527843553994893No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA4880.11273722754207433No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4800.11088907627089277No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4740.10950296281750661No Hit
CCCTCGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTG4710.10880990609081352No Hit
GGAGCACAACTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATA4690.10834786827301815No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4680.10811684936412044No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4670.10788583045522276No Hit
GTCCAAGAGTTCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4610.1064997170018366No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTC4610.1064997170018366No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4560.10534462245734813No Hit
GTTAAGGGAGTAGCCCCATCTTTTGGTTTTATTAGGGTTCAAATGTATAC4560.10534462245734813No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4560.10534462245734813No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4490.10372749009506428No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG4450.1028034144594735No Hit
GATTTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCCCTGG4420.10211035773278043No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4390.10141730100608735No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC4380.10118628209718965No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4370.10095526318829195No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4340.10026220646159889No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT4330.10003118755270118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)