FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483765
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT18610.3846909139768276No Hit
CCTCTACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17660.3650532800016537No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGGTC17370.3590586338408111No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG17340.3584384980310688No Hit
CTCCCTGACCGCCGATTGGTGGAGGCAGGAGGAGGAGCTGCTGGCACTGT17040.35223713993364547No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTGCCGG16840.3481029012020299No Hit
GTGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCTAAGC16790.34706934151912605No Hit
GTCAAGGAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATC16130.3334263537047947No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15460.3195766539538826No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA13750.28422891279856954No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12550.2594234804088762No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT12330.2548758178040991No Hit
GTTGTCAATGTGCCCTGAGCCTGGGGGCTCCACCCCAAAAGACCGCCGTG11770.24329994935557553No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC11710.24205967773609088No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11650.2408194061166062No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11590.2395791344971215No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT11270.23296435252653666No Hit
ACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA11010.22758984217543643No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT10910.22552272280962865No Hit
CTGTCGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGA10890.2251092989364671No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9400.19430922038593118No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG9380.19389579651276964No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC9240.19100182940063876No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG9070.18748772647876552No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8980.18562731904953853No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8890.18376691162031153No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA8830.18252664000082686No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG8470.17508501028391885No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8260.1707440596157225No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACACTGTGTAAAA8240.17033063574256097No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8140.16826351637675316No Hit
CCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAAC8050.16640310894752616No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7920.16371585377197606No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7870.16268229408907217No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7580.15668764792822962No Hit
CTCCTTGACTGCCGATTGGTGGAGGCAGGAGGAGGAGCTGCTGGCACTGT7540.1558608001819065No Hit
AGGTAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA7370.15234669726003328No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7260.15007286595764474No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAGACCACATC7130.1473856107820946No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6920.14304466011389827No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6780.14015069300176738No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAGACCACAT6720.1389104213822827No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA6680.1380835736359596No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6670.13787686169937885No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC6630.13705001395305572No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6590.1362231662067326No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT6560.13560303039699026No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6510.13456947071408637No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTGCTCTTCCAAAAGTAAGG6500.13436275877750561No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6430.13291577522144016No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6320.1306419439190516No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6320.1306419439190516No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG6310.13043523198247084No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6280.1298150961727285No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCTACCTTACAGACCACCAAATGCC6260.12940167229956695No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG6240.12898824842640538No Hit
GTCAGGGAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATC6230.1287815364898246No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6200.12816140068008228No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6200.12816140068008228No Hit
GGATTAAAGGTAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6190.1279546887435015No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA6140.1269211290605976No Hit
CTATTGTGGTTTCACATTTCCTGCCTTACTTTTGGAAGAGCAACTGTTCT6080.12568085744111293No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG6060.12526743356795136No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6010.12423387388504749No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5990.12382045001188595No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC5920.12237346645582049No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5890.12175333064607814No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGGAGTTCCACTGCATG5860.12113319483633583No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG5860.12113319483633583No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5830.12051305902659348No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5800.11989292321685115No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5700.11782580385104337No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5690.1176190919144626No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGGTCATT5680.11741237997788183No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCTACC5650.11679224416813949No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5620.11617210835839714No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5590.11555197254865482No Hit
CTCAAGAACAGTTGCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA5580.11534526061207405No Hit
CTATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5570.11513854867549325No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5570.11513854867549325No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5540.11451841286575094No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5490.11348485318284705No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGC5480.11327814124626626No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5420.1120378696267816No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5300.10955732638781225No Hit
GTACAGACTTGGCCCCCAAGACCACATCATCCATATAACTGAAAGCCAAA5290.10935061445123148No Hit
ATACAGCACTCAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTG5270.10893719057806994No Hit
AAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTAT5250.10852376670490838No Hit
GTATAAAGAATTTGGAGCTTCTGTCGAGTTACTCTCTTTTTTGCCTTCTG5240.10831705476832759No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5230.10811034283174682No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5180.10707678314884293No Hit
CTGTAAGGTAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC5160.10666335927568137No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5150.1064566473391006No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5130.10604322346593904No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5040.10418281603671205No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTATAAA5020.10376939216355048No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA5000.10335596829038893No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4990.10314925635380816No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGAGGACGAGGC4970.10273583248064659No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGGTCATTAACCTAATCTCCTCC4930.10190898473432349No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC4910.10149556086116193No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4900.10128884892458116No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4860.10046200117825804No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4850.10025528924167725No Hit
GTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCTTCAT4840.10004857730509648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)