FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483765
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTGCCGG17790.3677405351772038No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG17710.3660868396845576No Hit
CTCCCTGACCGCCGATTGGTGGAGGCAGGAGGAGGAGCTGCTGGCACTGT17280.3571982264115841No Hit
GTGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCTAAGC17070.3528572757433878No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT16830.34789618926544913No Hit
CCTCTACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16740.34603578183622213No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGGTC16640.34396866247041435No Hit
GTCAAGGAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATC14140.29229067832521993No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13900.28732959184728124No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA13370.2763738592085No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12300.2542556819943568No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12070.2495013074529989No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT11590.2395791344971215No Hit
GTTGTCAATGTGCCCTGAGCCTGGGGGCTCCACCCCAAAAGACCGCCGTG11380.2352381838289252No Hit
CTGTCGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGA11200.2315173689704712No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT11180.23110394509730964No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10960.2265562824925325No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10530.2176676692195591No Hit
ACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA10520.21746095728297832No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG10240.21167302305871652No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9410.19451593232251196No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9130.18872799809825017No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG8980.18562731904953853No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8850.1829400638739884No Hit
CTCCTTGACTGCCGATTGGTGGAGGCAGGAGGAGGAGCTGCTGGCACTGT8820.18231992806424607No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC8780.18149308031792294No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8610.17797897739604973No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA8600.17777226545946898No Hit
CCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAAC8550.17673870577656506No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG8450.17467158641075728No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8440.17446487447417652No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8380.17322460285469185No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACACTGTGTAAAA8320.17198433123520718No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8190.16929707605965705No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8110.16764338056701084No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7910.16350914183539528No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7520.15544737630874494No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG7440.15379368081609873No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7400.15296683306977563No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7100.14676547497235226No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTGCTCTTCCAAAAGTAAGG7090.1465587630357715No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAGACCACATC6900.14263123624073673No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6880.14221781236757516No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6830.14118425268467127No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6710.13870370944570196No Hit
AGGTAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA6700.13849699750912114No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT6680.1380835736359596No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG6620.13684330201647496No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6560.13560303039699026No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA6540.13518960652382872No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGGAGTTCCACTGCATG6470.13374262296776326No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6470.13374262296776326No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCTACCTTACAGACCACCAAATGCC6410.13250235134827862No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG6400.13229563941169784No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6380.13188221553853627No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6310.13043523198247084No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6280.1298150961727285No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6240.12898824842640538No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAGACCACAT6200.12816140068008228No Hit
GGATTAAAGGTAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6190.1279546887435015No Hit
CTATTGTGGTTTCACATTTCCTGCCTTACTTTTGGAAGAGCAACTGTTCT6080.12568085744111293No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6070.12547414550453215No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6030.12464729775820906No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCTACC6010.12423387388504749No Hit
GTCAGGGAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATC6000.12402716194846672No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5950.12299360226556283No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCCGGTC5830.12051305902659348No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5810.12009963515343194No Hit
ATACAGCACTCAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTG5730.11844593966078572No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5730.11844593966078572No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA5720.11823922772420493No Hit
CTCAAGAACAGTTGCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA5680.11741237997788183No Hit
CTGTAAGGTAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC5640.11658553223155871No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5550.11472512480233171No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5550.11472512480233171No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC5510.11389827705600859No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5510.11389827705600859No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5480.11327814124626626No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG5470.11307142930968549No Hit
GTATAAAGAATTTGGAGCTTCTGTCGAGTTACTCTCTTTTTTGCCTTCTG5440.11245129349994316No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG5430.11224458156336237No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5360.11079759800729694No Hit
GTACAGACTTGGCCCCCAAGACCACATCATCCATATAACTGAAAGCCAAA5340.11038417413413537No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5310.10976403832439305No Hit
AAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTAT5300.10955732638781225No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5290.10935061445123148No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5250.10852376670490838No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5230.10811034283174682No Hit
CTATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5200.10749020702200449No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT5190.1072834950854237No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5160.10666335927568137No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5110.10562979959277748No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5090.10521637571961594No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGC5080.10500966378303517No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5070.10480295184645437No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTATAAA5060.1045962399098736No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5010.10356268022696971No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCCCAGGCTCA4970.10273583248064659No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4960.10252912054406582No Hit
GTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGAT4930.10190898473432349No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGAGGACGAGGC4880.1008754250514196No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4880.1008754250514196No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4860.10046200117825804No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG4860.10046200117825804No Hit
GCACTCAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4850.10025528924167725No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCT4840.10004857730509648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)