FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423011
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC14110.3335610657878873No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11770.27824335537373734No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11660.27564295018332857No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC11640.2751701492396179No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11620.2746973482959072No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10990.2598041185690207No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGATAAAGGCATTAAACCTTAT10770.25460330818820315No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCT10620.251057301110373No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG9410.22245284401587664No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9180.21701563316320382No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT9150.21630643174763778No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8650.20448640815487065No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8540.2018860029644619No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8470.2002311996614745No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8450.19975839871776385No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8400.19857639635848714No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8290.19597599116807835No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT8060.1905387803154055No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7960.18817477559685208No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATATT7930.18746557418128607No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7870.18604717135015403No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7860.18581077087829867No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7750.18321036568788993No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7400.17493634917295295No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7180.16973553879213543No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7140.16878993690471406No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7050.166662332658016No Hit
ATTTCAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGA6790.1605159203897771No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT6700.15838831614307902No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6630.15673351284009163No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAAGCCTTGTAAGTTGGCGA6500.1536603067059722No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6370.15058710057185273No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6310.1491686977407207No Hit
CCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTATAT6240.1475138944377333No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6190.1463318920784566No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA6110.14444068830361387No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5910.139712678866507No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5890.13923987792279635No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5880.139003477450941No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT5800.13711227367609824No Hit
GCAGACGCAGAAGGGGACGGTAGAGGCCCAAACGGCCCCGAGACGGGTCG5780.13663947273238758No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5720.13522106990125551No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5650.13356626659826815No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG5620.1328570651827021No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG5450.1288382571611613No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5360.12671065291446323No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5350.12647425244260788No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5330.1260014514988972No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5320.12576505102704186No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5320.12576505102704186No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGGTGGCC5290.1250558496114758No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC5240.12387384725219912No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5200.12292824536477776No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5190.12269184489292241No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT5190.12269184489292241No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5120.12103704158993503No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5100.12056424064622433No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA5020.1186730368713816No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5010.11843663639952626No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4940.11678183309653888No Hit
GACACATCCAACGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG4940.11678183309653888No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4860.11489062932169612No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCTTTGGATAAAGGCATTAAAC4850.11465422884984078No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4840.11441782837798545No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4840.11441782837798545No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4800.11347222649056407No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4790.11323582601870874No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4780.1129994255468534No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4710.11134462224386601No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4600.10874421705345724No Hit
TTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC4580.10827141610974655No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCTTTTCTGTGTC4570.10803501563789121No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGAGGGCGTTC4530.10708941375046983No Hit
GGATGGGGCTTGGCCATAGGCCACCGGCGCATGCGTGGAACCTTTGTGGC4510.10661661280675916No Hit
TACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGC4490.10614381186304848No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4490.10614381186304848No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4480.10590741139119314No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT4480.10590741139119314No Hit
ACAAAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAA4470.10567101091933778No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA4470.10567101091933778No Hit
ATTTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGT4450.10519820997562711No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4420.10448900856006109No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4420.10448900856006109No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4410.10425260808820573No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4390.10377980714449506No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4320.10212500384150767No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4310.10188860336965233No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAAGGCAAATATTTGGTAAGGTT4300.101652202897797No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT4280.1011794019540863No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4270.10094300148223095No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4250.10047020053852028No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4250.10047020053852028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)