FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615912
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT24110.39145202561404874No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG19390.3148177012300459No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19380.31465534037330006No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16930.2748769304705867No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16800.27276623933289174No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC15930.25864084479600985No Hit
GTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14890.24175531569444986No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14840.24094351141072098No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13530.21967423917702528No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG12990.2109067529127538No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12920.20977022691553338No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG12830.20830897920482144No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12720.20652300978061802No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT12680.20587356635363493No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA12300.1997038537972957No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG12220.19840496694332957No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA11330.18395485069295614No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA11320.18379248983621035No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11060.17957110756082037No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT10890.1768109729961423No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10550.17129070386678616No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10170.16512099131044694No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9820.15943836132434502No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9490.15408045305173465No Hit
ATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCCGGAACATGCAG9360.15196976191403966No Hit
TCCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9230.14985907077634467No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9090.14758601878190392No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA9080.14742365792515816No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8870.144014079933497No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8760.14222811050929351No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG8600.13963033680136125No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCATCAAGCTCTGCTAG8600.13963033680136125No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG8540.13865617166088662No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCTAACGTACAAGGCC8340.13540895452597124No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8310.13492187195573394No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA8280.13443478938549663No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC8200.1331359025315305No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8160.1324864591045474No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCGGCA8110.13167465482081855No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA8100.13151229396407277No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8080.13118757225058125No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8060.1308628505370897No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8010.1300510462533609No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGGACAGTAAACCCTGTTCCGA8010.1300510462533609No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7960.12923924196963202No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC7880.12794035511566587No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7740.12566730312122512No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG7710.1251802205509878No Hit
TTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACAC7630.12388133369702166No Hit
GCCTGAGGATGACTGTCTCTTAGAGGAGGAGAGATGGGAGTAGGCTGTCT7610.12355661198353012No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGACCGAATGCTCCCA7580.1230695294132928No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7360.11949759056488589No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT7310.11868578628115704No Hit
GGTAAATATTTGGTAATGTTAGGATAAAACCTAGCAGGCATAATTAATTT7290.1183610645676655No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7290.1183610645676655No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT7250.11771162114068243No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7230.1173868994271909No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7130.11576329085973322No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGATGGAATGTGGTGG7110.11543856914624168No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7090.11511384743275015No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC7080.11495148657600436No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC7000.11365259972203823No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC6990.11349023886529244No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6950.11284079543830937No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6920.11235371286807205No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6880.11170426944108898No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6850.11121718687085166No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6740.10943121744664822No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6640.10780760887919054No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6560.10650872202522439No Hit
GTGTGTAGTTTCTCTCTTATATAGAATACCAGCCTTCCACAGAGTATGTA6550.1063463611684786No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6480.10520983517125823No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6470.10504747431451247No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6470.10504747431451247No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6450.10472275260102093No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG6350.10309914403356323No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTACGTTAGCGA6340.10293678317681747No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT6340.10293678317681747No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6320.10261206146332594No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6290.10212497889308862No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC6250.10147553546610555No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT6240.10131317460935978No Hit
GATTTTTAGGCCCATATTAACGTTGACATAGCTGACTACTAATTCCCTGG6230.10115081375261402No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6230.10115081375261402No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA6220.10098845289586823No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTCAACAAAC6220.10098845289586823No Hit
CTGTCTCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATAC6180.10033900946888516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)