FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215992
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA13990.6477091744138672No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12990.6014111633764214No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9310.43103448275862066No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT7190.33288269935923553No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT6390.29584429052927885No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6110.28288084743879405No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA5800.26852846401718583No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5730.26528760324456463No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5620.2601948220304456No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5600.25926886180969666No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG5110.2365828364013482No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTTTGACATACT5060.2342679358494759No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC5040.233341975628727No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC4940.2287121745249824No Hit
TGCATGTTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACG4760.22037853253824216No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4620.21389681099299976No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4550.21065595022037856No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4540.21019297011000407No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4390.2032482684543872No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4390.2032482684543872No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4370.20232230823363828No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA4360.20185932812326382No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4350.2013963480128894No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG4090.18935886514315345No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4080.188895885032779No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4050.18750694470165563No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3870.17917330271491536No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3820.17685840216304308No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3750.17361754139042185No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT3740.17315456128004741No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3720.1722286010592985No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3630.16806178006592837No Hit
CTGTATAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGG3580.1657468795140561No Hit
GTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCTGCGTT3520.16296899885180932No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3520.16296899885180932No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3440.15926515796881366No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG3400.1574132375273158No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG3390.15695025741694135No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3360.155561317085818No Hit
AGACCTGCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGC3280.15185747620282233No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3260.1509315159820734No Hit
GCATGTTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGG3230.14954257565095003No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA3200.14815363531982664No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA3190.1476906552094522No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGCAGATGAGGCATAGCAGCAGG3160.14630171487832883No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3150.14583873476795436No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC3130.14491277454720544No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA3080.14259787399533316No Hit
GTGCAGTTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAG3070.1421348938849587No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3060.14167191377458424No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC3050.14120893366420978No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3020.13981999333308642No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC3010.13935701322271193No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2990.13843105300196304No Hit
TGGATAAAGGCATTAAACCGTATTATCCTGAAAATGCAGTTAATCATTAC2930.1356531723397163No Hit
GTCCAGAAGAACCAACAAGCAGATGAGGCATAGCAGCAGGATGAAGAGGA2890.13380125189821845No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG2870.13287529167746953No Hit
GCAGACGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCC2870.13287529167746953No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT2850.1319493314567206No Hit
ATAGAGACCCAACCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG2840.13148635134634615No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT2820.13056039112559725No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2810.1300974110152228No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA2800.12963443090484833No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2800.12963443090484833No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT2780.1287084706840994No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG2730.12639357013222713No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2730.12639357013222713No Hit
GGACCATGCAAGAACTGCACAACTCCTGCTCAAGGAACCTCTATGTTTCC2720.12593059002185264No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC2700.12500462980110374No Hit
CCTTTCTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG2680.12407866958035482No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT2650.12268972924923144No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA2630.12176376902848253No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACTTGGGTGGGAAGTAATTTGG2630.12176376902848253No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2610.12083780880773362No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2600.12037482869735915No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG2530.11713396792473794No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA2510.11620800770398904No Hit
CCCCTATCCTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC2500.11574502759361459No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAG2450.1134301270417423No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA2440.11296714693136782No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC2440.11296714693136782No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT2410.11157820660024445No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG2410.11157820660024445No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2410.11157820660024445No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA2410.11157820660024445No Hit
GGAGTAGGCTGTCTCCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGTGC2400.11111522648987No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC2390.11065224637949553No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT2370.10972628615874662No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT2370.10972628615874662No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC2340.10833734582762324No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG2330.10787436571724879No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2330.10787436571724879No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG2330.10787436571724879No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT2320.10741138560687433No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG2300.10648542538612542No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG2280.1055594651653765No Hit
GGATAAAGGCATTAAACCGTATTATCCTGAAAATGCAGTTAATCATTACT2280.1055594651653765No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA2280.1055594651653765No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC2260.10463350494462759No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCCTGGTGGAATGTTGTGG2250.10417052483425313No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT2240.10370754472387865No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC2210.1023186043927553No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC2180.10092966406163191No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT2180.10092966406163191No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTCCCTGACTGCCGATTG2180.10092966406163191No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA2180.10092966406163191No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2170.10046668395125745No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA2160.100003703840883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)