FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215992
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA11960.5537242120078522No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11040.511130041853402No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9890.4578873291603393No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG9030.41807103966813586No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6630.30695581317826587No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT6510.3014000518537724No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5900.2731582651209304No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT5680.2629727026926923No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5490.2541760805955776No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG5330.24676839882958626No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTTTGACATACT5260.24352753805696506No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA5170.23936071706359496No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4820.2231564132004889No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4530.2097299899996296No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4460.2064891292270084No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4300.19908144746101705No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4290.19861846735064262No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4190.19398866624689803No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA4150.19213674580540022No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4120.19074780547427683No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4080.188895885032779No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC4000.18519204414978332No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3960.1833401237082855No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3880.17963628282528982No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC3840.177784362383792No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT3720.1722286010592985No Hit
TGCATGTTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACG3720.1722286010592985No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3710.17176562094892403No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG3690.1708396607281751No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3650.1689877402866773No Hit
GTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCTGCGTT3580.1657468795140561No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3490.16158005852068594No Hit
CTGTATAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGG3390.15695025741694135No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3380.15648727730656692No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3260.1509315159820734No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3240.1500055557613245No Hit
AGACCTGCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGC3230.14954257565095003No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3200.14815363531982664No Hit
TGGATAAAGGCATTAAACCGTATTATCCTGAAAATGCAGTTAATCATTAC3170.1467646949887033No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3170.1467646949887033No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG3110.14398681432645652No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3080.14259787399533316No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA3060.14167191377458424No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA3060.14167191377458424No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA3060.14167191377458424No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3000.1388940331123375No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGCAGATGAGGCATAGCAGCAGG2990.13843105300196304No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT2950.1365791325604652No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA2940.13611615245009073No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2940.13611615245009073No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT2880.133338271787844No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT2860.1324123115670951No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT2850.1319493314567206No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2820.13056039112559725No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA2780.1287084706840994No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2770.12824549057372495No Hit
GTCCAGAAGAACCAACAAGCAGATGAGGCATAGCAGCAGGATGAAGAGGA2750.12731953035297602No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC2730.12639357013222713No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC2680.12407866958035482No Hit
CCTTTCTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG2680.12407866958035482No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG2670.12361568946998037No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTG2660.12315270935960591No Hit
ATAGAGACCCAACCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG2640.122226749138857No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA2620.12130078891810808No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG2620.12130078891810808No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC2610.12083780880773362No Hit
CCCCTATCCTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC2580.11944886847661024No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA2570.11898588836623579No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA2530.11713396792473794No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC2530.11713396792473794No Hit
GCATGTTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGG2480.11481906737286565No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACTTGGGTGGGAAGTAATTTGG2480.11481906737286565No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC2480.11481906737286565No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG2470.1143560872624912No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT2450.1134301270417423No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCCTGGTGGAATGTTGTGG2440.11296714693136782No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC2440.11296714693136782No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2430.11250416682099337No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA2420.11204118671061891No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2420.11204118671061891No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG2390.11065224637949553No Hit
GTGCAGTTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAG2390.11065224637949553No Hit
GGAGTAGGCTGTCTCCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGTGC2360.10926330604837216No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT2330.10787436571724879No Hit
GCAGACGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCC2330.10787436571724879No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC2320.10741138560687433No Hit
GTGTCAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC2300.10648542538612542No Hit
GGACCATGCAAGAACTGCACAACTCCTGCTCAAGGAACCTCTATGTTTCC2300.10648542538612542No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGTCCGAATGCTCCCG2290.10602244527575094No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG2290.10602244527575094No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC2260.10463350494462759No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2260.10463350494462759No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2260.10463350494462759No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2250.10417052483425313No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2230.1032445646135042No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA2220.10278158450312974No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT2210.1023186043927553No Hit
CTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA2200.10185562428238082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)