FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356290
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15420.43279351090403884No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT14610.4100592214207528No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG11820.33175222431165624No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11700.3283841814252435No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11420.3205254146902804No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10850.30452721097981983No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT10330.2899323584720312No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9840.2761795166858458No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9060.25428723792416286No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9010.2528838867214909No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8960.25148053551881894No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8380.23520166156782396No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8220.23071093771927362No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8190.22986892699767042No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7980.22397485194644812No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7890.22144881978163855No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7770.21808077689522581No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7600.21330938280614106No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7590.21302871256560665No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7070.19843386005781805No Hit
GTCTTTGAAGTATGCCTCCAGGTCAGTTGTTGACATTGCTGAGAGTCCAA7030.1973111790956805No Hit
CTGTAAGAGGACCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6910.19394313620926773No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6600.18524235875270145No Hit
ATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGC6540.18355833730949508No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6440.1807516349041511No Hit
GGTTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG6400.17962895394201353No Hit
TGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCAT6350.17822560273934154No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6080.17064750624491287No Hit
CTACCGCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGA5720.16054337758567458No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5650.1585786859019338No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5560.15605265373712426No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5430.1524039406101771No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5430.1524039406101771No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5380.15100058940750513No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5330.14959723820483314No Hit
GGGTAAAGGTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5230.14679053579948917No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5230.14679053579948917No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG5220.1465098655589548No Hit
GTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5140.14426450363467963No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5070.14229981195093885No Hit
GGATTAAAGACAGGTACAGTGGAAGAATAAAGCCCAGTAAAGTTTCCCAC4980.13977377978612926No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG4930.1383704285834573No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4920.1380897583429229No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG4910.1378090881023885No Hit
ATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4910.1378090881023885No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4820.13528305593757894No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4740.13303769401330376No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4660.1307923320890286No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4590.12882764040528782No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4560.12798562968368463No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4530.12714361896208143No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4510.12658227848101267No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4500.12630160824047826No Hit
GTAGAGGCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4450.12489825703780627No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4430.1243369165567375No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4350.12209155463246232No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4330.12153021415139352No Hit
ATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAG4320.12124954391085914No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4250.11928485222711836No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4240.11900418198658397No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4240.11900418198658397No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4220.11844284150551516No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4130.11591680934070561No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4110.11535546885963681No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4100.11507479861910241No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4070.11423278789749923No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4040.11339077717589603No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG4030.11311010693536164No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4020.11282943669482726No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC3960.11114541525162086No Hit
GTGTAAACAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3950.11086474501108648No Hit
CAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCA3940.11058407477055208No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA3930.11030340453001768No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT3890.10918072356788008No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG3890.10918072356788008No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG3880.1089000533273457No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC3850.1080580426057425No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3780.10609335092200173No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA3740.10497066995986415No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC3720.10440932947879536No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3690.10356731875719218No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC3690.10356731875719218No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA3630.1018832973139858No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG3580.10047994611131382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)