FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356290
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15230.4274607763338853No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT13600.38171152712677875No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG12020.3373656291223442No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11080.3109826265121109No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11040.30985994554997337No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9220.2587779617727133No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9170.25737461057004124No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9090.2551292486457661No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8930.25063852479721577No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8750.24558646046759663No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8220.23071093771927362No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8200.23014959723820483No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8000.2245361924275169No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7840.22004546857896656No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7610.21359005304667547No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7460.2093799994386595No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7320.20545061607117795No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7250.2034859243874372No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7010.1967498386146117No Hit
ATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGC6950.1950658171714053No Hit
CTGTAAGAGGACCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6920.19422380644980214No Hit
GTCTTTGAAGTATGCCTCCAGGTCAGTTGTTGACATTGCTGAGAGTCCAA6730.1888910718796486No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6390.17934828370147912No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6280.17626091105560077No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6200.1740155491313256No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6190.17373487889079123No Hit
TGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCAT6130.17205085744758483No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5760.16166605854781219No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5730.160824047826209No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5650.1585786859019338No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5550.15577198349658986No Hit
GGGTAAAGGTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5500.15436863229391787No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG5490.1540879620533835No Hit
CTACCGCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGA5330.14959723820483314No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5240.14707120604002358No Hit
GTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5210.14622919531842038No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5130.14398383339414522No Hit
CCGCCGACACATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTG5050.14173847146987004No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4920.1380897583429229No Hit
ATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4920.1380897583429229No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4850.13612506665918211No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG4850.13612506665918211No Hit
GGATTAAAGACAGGTACAGTGGAAGAATAAAGCCCAGTAAAGTTTCCCAC4830.13556372617811333No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4670.13107300232956298No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4570.12826629992421903No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4560.12798562968368463No Hit
ATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAG4490.12602093799994385No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4440.12461758679727189No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4430.1243369165567375No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4410.12377557607566869No Hit
GTAGAGGCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4350.12209155463246232No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4310.12096887367032474No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4310.12096887367032474No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4280.12012686294872155No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4230.11872351174604956No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4180.11732016054337759No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4160.1167588200623088No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4140.11619747958123999No Hit
GGTTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG4130.11591680934070561No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4110.11535546885963681No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4100.11507479861910241No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4060.11395211765696484No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4040.11339077717589603No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG3980.11170675573268966No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3930.11030340453001768No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT3880.1089000533273457No Hit
CAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCA3820.10721603188413933No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3820.10721603188413933No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3820.10721603188413933No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3810.10693536164360493No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3810.10693536164360493No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG3750.10525134020039856No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3730.10468999971932975No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3690.10356731875719218No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3690.10356731875719218No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA3650.10244463779505458No Hit
ATATGTGGGTCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTA3630.1018832973139858No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT3570.10019927587077943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)