FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435767
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA25000.5737010833771259No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC22700.5209205837064304No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG22330.5124298076724488No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG19060.4373897059667208No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA18630.4275220473326342No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13350.3063563785233852No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11130.2554117223194964No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG10810.24806834845226924No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10510.2411839354517437No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG10350.23751224851813008No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10110.23200471811770967No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9940.22810355075074526No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCGGG9890.226956148583991No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9740.2235139420837282No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9500.2180064116833078No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA8720.2001069378819415No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7940.18220746408057517No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7850.18014214018041752No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7830.17968317931371583No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA7750.177847335846909No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7710.1769294141135056No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7580.17394616847994454No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7480.17165136414643606No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7460.17119240327973434No Hit
GGATACTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCA7460.17119240327973434No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7430.1705039619796818No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7240.16614383374601566No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG7180.16476695114591053No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6830.15673513597863079No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6720.15421085121177142No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6680.15329292947836803No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6660.15283396861166634No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6630.15214552731161377No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6610.15168656644491207No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6600.15145708601156121No Hit
GCATGGGAGGTTGGTCCTCCAAACCTCGACAAGGCATGGGGACGAATCTT6520.14962124254475442No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6500.14916228167805273No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6470.14847384037800015No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6460.14824435994464932No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT6310.14480215344438654No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6280.144113712144334No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT6270.14388423171098316No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6240.1431957904109306No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6190.14204838824417637No Hit
CCCAAAGACAGAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG6170.14158942737747465No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT6130.14067150564407127No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGAGATCTCCTCGACACC6100.13998306434401872No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6040.1386061817439136No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5990.13745877957715935No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA5900.1353934556770017No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC5830.13378709264354577No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5760.13218072961008978No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT5750.13195124917673895No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5720.13126280787668637No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5540.1271321600763711No Hit
GTAGATGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5490.12598475790961683No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5430.12460787530951174No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5400.12391943400945919No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5390.12368995357610833No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG5290.12139514924259982No Hit
ATTGACAACCGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5270.12093618837589813No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5220.11978878620914388No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC5180.11887086447574047No Hit
GCCACTCAGGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAG5180.11887086447574047No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAAACTCCTCC5170.11864138404238961No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5100.11703502100893366No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5060.11611709927553027No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5060.11611709927553027No Hit
TCCTAGTACAAAGACCTTTAACCTAAACTCCTCCCCCAACTCCTCCCAGT5040.11565813840882858No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5010.11496969710877603No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5000.11474021667542518No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5000.11474021667542518No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5000.11474021667542518No Hit
GTTTGGAGGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG4960.11382229494202177No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4940.11336333407532007No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG4920.11290437320861837No Hit
GTTCAAATGTATACCCAAAGACAGAAGAAAATTGGTAATAGAGGTAAAAA4920.11290437320861837No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4900.11244541234191666No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG4850.11129801017516243No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAGAGGACAACAGAGTTGTCAGTC4840.11106852974181157No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4810.11038008844175902No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA4810.11038008844175902No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA4700.10785580367489965No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4690.10762632324154882No Hit
ATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4680.10739684280819796No Hit
CCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCC4680.10739684280819796No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4680.10739684280819796No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4660.10693788194149625No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4650.10670840150814541No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC4650.10670840150814541No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4640.10647892107479456No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4630.1062494406414437No Hit
ACAATGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACT4610.10579047977474201No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4600.10556099934139115No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4540.10418411674128607No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4460.10234827327447926No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA4390.10074191024102329No Hit
TTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC4380.10051242980767246No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4370.1002829493743216No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG4370.1002829493743216No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4360.10005346894097075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)